spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kirkland, S. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kirkland, S. C.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Journal of Cell Science, Vol 91, Issue 4 615-621, Copyright © 1988 by Company of Biologists

JOURNAL ARTICLES

Polarity and differentiation of human rectal adenocarcinoma cells in suspension and collagen gel cultures

SC Kirkland
Department of Histopathology, Royal Postgraduate Medical School, Hammersmith Hospital, London.

HRA-19a1.1. cells, derived from a primary human rectal adenocarcinoma, form polarized monolayers when grown on tissue-culture plastic. The apical membrane of the cells is in contact with the culture medium while the basolateral surface is attached to the plastic substratum. Cells cultured on non-tissue-culture plastic form floating colonies. Cells within these colonies are orientated so that their apical membrane is in contact with the culture medium while the basolateral membrane faces the centre of the colony. When these colonies are embedded in collagen gel the cells organize to form glandular structures similar to those observed in xenografts of this cell line. In addition a reversal in the orientation of cell polarity is observed with the basolateral membrane now in contact with the collagen gel, while the apical membrane faces lumina within the colony. This interaction is specific to collagen gel, as in a control experiment where colonies are embedded in agarose gel, neither a reversal in polarity nor the formation of glandular structures is observed. These results demonstrate an induction of glandular organization in human rectal adenocarcinoma cells by collagen gel. In addition an increased absorptive cell differentiation and reversal in cell polarity is observed in response to collagen gel.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 J. Clin. Pathol.Home page
S A Adams, M E F Smith, G P Cowley, and L A Carr
Reversal of glandular polarity in the lymphovascular compartment of breast cancer
J. Clin. Pathol., October 1, 2004; 57(10): 1114 - 1117.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
T. Gudjonsson, L. Ronnov-Jessen, R. Villadsen, F. Rank, M. J. Bissell, and O. W. Petersen
Normal and tumor-derived myoepithelial cells differ in their ability to interact with luminal breast epithelial cells for polarity and basement membrane deposition
J. Cell Sci., January 1, 2002; 115(1): 39 - 50.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
S. Kirkland and K Henderson
Endocrine and mucous differentiation by a cloned human rectal adenocarcinoma cell line (HRA-19) in vitro: inhibition by TGF-beta 1
J. Cell Sci., January 4, 1994; 107(4): 1041 - 1046.
[Abstract] [PDF]


© The Company of Biologists Ltd 1988