spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by LANE, N. J.
Right arrow Articles by DILWORTH, S. M.
Right arrow Search for Related Content
PubMed
Right arrow Articles by LANE, N. J.
Right arrow Articles by DILWORTH, S. M.

Journal of Cell Science, Vol 93, 123-131, Copyright © 1989 by Company of Biologists

Submitted on October 18, 1988
Accepted on January 31, 1989

Isolation and biochemical characterization of septate junctions: differences between the proteins in smooth and pleated varieties

NANCY J. LANE 1 and STEPHEN M. DILWORTH 2

1 AFRC Unit of Insect Neurophysiology and Pharmacology, Department of Zoology Downing Street, Cambridge CB2 3EJ, UK
2 CRC Molecular Embryology Group, Department of Zoology Downing Street, Cambridge CB2 3EJ, UK

Septate junctions are found only in invertebrate tissues, and are almost ubiquitous within them. In arthropods, the two major types are the ‘pleated’ and the ‘smooth’ varieties. Using tissues from different species, including the cockroach Periplaneta americana, procedures have been established for obtaining membrane fractions selectively enriched in septate junctions. The junctions have been identified in pellets of these fractions by both thin sectioning and freeze-fracturing. SDS-PAGE of these membrane fractions reveals two major polypeptide species with apparent molecular weights of 22000-24000 and 17000-18000. Consistent differences in these apparent molecular weights are observed between the pleated and smooth varieties of septate junction. These polypeptides are probably integral membrane components, as they remain associated after treatment with high concentrations of urea. Evidence suggests a plane of weakness in the mid-line of the extracellular septal ribbons.

Key words: septate junctions, arthropod tissues, insect gut, junctional membrane proteins

Submitted on October 18, 1988
Accepted on January 31, 1989




This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
M. E. Finbow, S. F. Goodwin, L. Meagher, N. J. Lane, J. Keen, J. B. Findlay, and K. Kaiser
Evidence that the 16 kDa proteolipid (subunit c) of the vacuolar H(+)-ATPase and ductin from gap junctions are the same polypeptide in Drosophila and Manduca: molecular cloning of the Vha16k gene from Drosophila
J. Cell Sci., July 1, 1994; 107(7): 1817 - 1824.
[Abstract] [PDF]


© The Company of Biologists Ltd 1989