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Journal of Cell Science, Vol 93, 385-389, Copyright © 1989 by Company of Biologists

Submitted on December 7, 1988
Accepted on March 17, 1989

Computer-generated three-dimensional representation of dictyosomes reconstructed from electron micrographs of serial ultrathin sections

U. KRISTEN 1, J. LOCKHAUSEN 1, W. MENHARDT 2, and W. J. DALLAS 3

1 Institut für Allgemeine Botanik, Universität Hamburg, Ohnhorststrasse 18, D-2000 Hamburg 52, West Germany
2 Philips GmbH, Forschungslabor Hamburg, Postfach 540840, D-2000 Hamburg 54, West Germany
3 Department of Radiology, University of Arizona, Health Sciences Center, Tucson, Arizona 85724, USA

Author for correspondence

Computer-generated three-dimensional reconstructions of higher plant dictyosomes from electron micrographs are presented from different viewing angles in the form of shaded perspective displays. The image processing has revealed complicated structures indicating a central cisternal stack surrounded by a cloud of vesicles and vesicular and tubular protrusions. The entire dictyosome is suggested as being an approximately spherical body that is marginally penetrated by plates and tubules of the endoplasmic reticulum.

Key words: Golgi apparatus, three-dimensional reconstruction, computer image display

Submitted on December 7, 1988
Accepted on March 17, 1989







© The Company of Biologists Ltd 1989