spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow A correction has been published
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Palmberg, L.
Right arrow Articles by Thyberg, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Palmberg, L.
Right arrow Articles by Thyberg, J.

Journal of Cell Science, Vol 93, Issue 3 403-408, Copyright © 1989 by Company of Biologists

JOURNAL ARTICLES

Effects of leukotrienes on phenotypic properties and growth of arterial smooth muscle cells in primary culture

L Palmberg, HE Claesson and J Thyberg
Department of Medical Cell Biology, Karolinska Institutet, Stockholm, Sweden.

During the first few days in primary culture arterial smooth muscle cells (SMCs) go through a transition from a contractile to a synthetic phenotype. Morphologically, this process includes loss of myofilaments and formation of an extensive rough endoplasmic reticulum and a large Golgi complex. Functionally, it leads to the cells losing their contractility, beginning to secrete extracellular matrix components, and dividing in response to growth factor stimulation. Similar changes in the structure and function of the SMCs occur in the initial stages of atherogenesis. The object of the present investigation was to study the effects of leukotrienes on the differentiated properties and growth of rat aortic SMCs in primary culture. Enzymically isolated cells were seeded directly on a plastic surface in serum-containing medium or on a substratum of plasma fibronectin in serum-free medium. The change in cell morphology was followed by transmission electron microscopy, and the activation of cell growth by thymidine autoradiography and cell counting. The results demonstrate that 10 pM-LTB4, -LTC4, -LTD4 and -LTE4 all speeded up the shift of the SMCs into a synthetic phenotype, whereas 5S,12S-DHETE (an isomer of LTB4) lacked effect. Further, LTB4, LTC4 and LTD4 stimulated the SMCs to enter the cell cycle earlier than in the controls, enhanced the proliferative response to serum mitogens, and under serum-free conditions induced DNA synthesis by themselves. Indomethacin did not interfere with the effect of LTB4 on the structural transformation of the cells but blocked its effect on DNA replication, suggesting that only the latter involved endogenous production of a cyclo-oxygenase product.(ABSTRACT TRUNCATED AT 250 WORDS)


This article has been cited by other articles:


Home page
 Circ. Res.Home page
Y. Wang and P. T. Kovanen
Heparin Proteoglycans Released From Rat Serosal Mast Cells Inhibit Proliferation of Rat Aortic Smooth Muscle Cells in Culture
Circ. Res., January 22, 1999; 84(1): 74 - 83.
[Abstract] [Full Text] [PDF]

Home page
 Ann. Thorac. Surg.Home page
J. Y. Jeremy, M. B. Izzat, S. D. Birkett, D. M. Knight, A. J. Bryan, and G. D. Angelini
Reduced Prostacyclin and Increased Leukotriene B4 Synthesis in Porcine Venous-Arterial Grafts
Ann. Thorac. Surg., January 1, 1996; 61(1): 143 - 148.
[Abstract] [Full Text]


© The Company of Biologists Ltd 1989