|
|
|
|
|
||
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 94, Issue 1 101-108, Copyright © 1989 by Company of Biologists
JOURNAL ARTICLES |
DJ Donaldson, JT Mahan, D Amrani and J Hawiger
Department of Anatomy and Neurobiology, Health Science Center, University of Tennessee, Memphis 38163.
Previously we showed that epidermal cells are able to use fibrinogen (FGN) as a migration substratum during wound closure. The goal of the present study was to determine the structural features of FGN that allow this migration. Pieces of glass coated with native, fragmented, or other modified forms of FGN were implanted into full-thickness skin wounds of adult newts such that migrating epidermal cells would encounter the implant. In this system, a coating of FGN allowed considerably more migration than a coating of BSA. At high concentrations, heat-denatured FGN supported as much migration as the same amount of intact FGN. Fraction I-9, a circulating form of FGN missing a 20-30K (K = 10(3) Mr) carboxy-terminal segment of the A alpha chain, was no less effective than intact FGN. Comparison of the isolated D1 and E fragments of FGN showed migration only on D1, but never to the extent seen on intact FGN containing the same amount of D1. Plasmin digestion of D1 in the presence of EDTA, a process which produces D3, a fragment differing from D1 by the loss of the carboxy-terminal 109 amino acids of the gamma chain, caused a significant loss of activity in the D fragment. Migration was good on implants coated with relatively high concentrations of purified A alpha chains but gamma chains were inactive. Migration over intact FGN was almost totally blocked by 230 microM-Arg-Gly-Asp-Ser (RGDS), a peptide known to interact with integrin-type receptors.(ABSTRACT TRUNCATED AT 250 WORDS)
This article has been cited by other articles:
|
|
 |
|
  H. O. Duan and P. J. Simpson-Haidaris Cell Type-specific Differential Induction of the Human {gamma}-Fibrinogen Promoter by Interleukin-6 J. Biol. Chem., May 5, 2006; 281(18): 12451 - 12457. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Pereira, B. J. Rybarczyk, T. M. Odrljin, D. C. Hocking, J. Sottile, and P. J. Simpson-Haidaris The incorporation of fibrinogen into extracellular matrix is dependent on active assembly of a fibronectin matrix J. Cell Sci., January 2, 2002; 115(3): 609 - 617. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. G. Sitrin, P. M. Pan, S. Srikanth, and R. F. Todd III Fibrinogen Activates NF-{kappa}B Transcription Factors in Mononuclear Phagocytes J. Immunol., August 1, 1998; 161(3): 1462 - 1470. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Guadiz, L. A. Sporn, R. A. Goss, S. O. Lawrence, V. J. Marder, and P. J. Simpson-Haidaris Polarized Secretion of Fibrinogen by Lung Epithelial Cells Am. J. Respir. Cell Mol. Biol., July 1, 1997; 17(1): 60 - 69. [Abstract] [Full Text]
|
|
|
|
 |
|
 P. J. Simpson Haidaris Induction of Fibrinogen Biosynthesis and Secretion From Cultured Pulmonary Epithelial Cells Blood, February 1, 1997; 89(3): 873 - 882. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. B. Milam, C. Haskin, G. Zardeneta, Dali Chen, V. L. Magnuson, R. J. Klebe, and B. Steffenson Cell Adhesion Proteins in Oral Biology Critical Reviews in Oral Biology & Medicine, January 1, 1991; 2(4): 451 - 491. [Full Text] [PDF]
|
|
