|
|
|
|
|
||
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 94, Issue 2 207-216, Copyright © 1989 by Company of Biologists
JOURNAL ARTICLES |
M Baba, N Baba, Y Ohsumi, K Kanaya and M Osumi
Department of Biology, Japan Women's University, Tokyo.
Ultrastructural analyses of cytoplasmic changes in Saccharomyces cerevisiae X2180-1A (MATa) that had been treated with alpha factor were performed by using the freeze-substitution fixation method. After alpha factor treatment, cells exhibited a pointed projection, which is a unique pattern of oriented cell surface growth. The relationship between projection formation and intracellular organelles was examined using serial thin sections and computer-aided three-dimensional reconstructions. Using these analyses membrane vesicles and other organelles were detected, and studies on their dynamic structural reorganization became feasible. Production of membrane vesicles (average 65 nm in diameter) was induced upon exposure of the cells to alpha factor before projection emergence. The total number of membrane vesicles increased at the early stage and decreased at the late stage of projection formation. Three-dimensional analysis indicated that the vesicles were at first dispersed throughout the cell, then accumulated at the site where the projection formed. Morphological changes and multiplication of the Golgi body were seen during the process of projection formation. Other intracellular organelles (nucleus, vacuole, rough endoplasmic reticulum and mitochondria) were also rearranged, showing a polar organization of the cytoplasm during projection formation.
This article has been cited by other articles:
|
|
 |
|
  S. Barale, D. McCusker, and R. A. Arkowitz Cdc42p GDP/GTP Cycling Is Necessary for Efficient Cell Fusion during Yeast Mating Mol. Biol. Cell, June 1, 2006; 17(6): 2824 - 2838. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. M. Harold Molecules into Cells: Specifying Spatial Architecture Microbiol. Mol. Biol. Rev., December 1, 2005; 69(4): 544 - 564. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Casolari, C. R. Brown, D. A. Drubin, O. J. Rando, and P. A. Silver Developmentally induced changes in transcriptional program alter spatial organization across chromosomes Genes & Dev., May 15, 2005; 19(10): 1188 - 1198. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Barale, D. McCusker, and R. A. Arkowitz The Exchange Factor Cdc24 Is Required for Cell Fusion during Yeast Mating Eukaryot. Cell, August 1, 2004; 3(4): 1049 - 1061. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Tada, M. Ohmori, and H. Iida Molecular Dissection of the Hydrophobic Segments H3 and H4 of the Yeast Ca2+ Channel Component Mid1 J. Biol. Chem., March 7, 2003; 278(11): 9647 - 9654. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. W. Rossanese, C. A. Reinke, B. J. Bevis, A. T. Hammond, I. B. Sears, J. O'Connor, and B. S. Glick A Role for Actin, Cdc1p, and Myo2p in the Inheritance of Late Golgi Elements in Saccharomyces cerevisiae J. Cell Biol., March 26, 2001; 153(1): 47 - 62. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Nern and R. A. Arkowitz A Cdc24p-Far1p-Gbeta gamma Protein Complex Required for Yeast Orientation during Mating J. Cell Biol., March 22, 1999; 144(6): 1187 - 1202. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. S. Karpova, J. G. McNally, S. L. Moltz, and J. A. Cooper Assembly and Function of the Actin Cytoskeleton of Yeast: Relationships between Cables and Patches J. Cell Biol., September 21, 1998; 142(6): 1501 - 1517. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. E. Gammie, V. Brizzio, and M. D. Rose Distinct Morphological Phenotypes of Cell Fusion Mutants Mol. Biol. Cell, June 1, 1998; 9(6): 1395 - 1410. [Abstract] [Full Text]
|
|
|
|
|
|
J. Philips and I. Herskowitz Osmotic Balance Regulates Cell Fusion during Mating in Saccharomyces cerevisiae J. Cell Biol., September 8, 1997; 138(5): 961 - 974. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Kuchler, H.M. Goransson, M.N. Viswanathan, and J. Thorner Dedicated Transporters for Peptide Export and Intercompartmental Traffic in the Yeast Saccharomyces cerevisiae Cold Spring Harb Symp Quant Biol, January 1, 1992; 57(0): 579 - 592. [Abstract] [PDF]
|
|
