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Journal of Cell Science, Vol 94, Issue 2 217-226, Copyright © 1989 by Company of Biologists

JOURNAL ARTICLES

The localization of the divergent beta 2-tubulin isotype in the microtubular arrays of Physarum polycephalum

M Diggins-Gilicinski, L Solnica-Krezel, TG Burland, EC Paul and WF Dove
McArdle Laboratory for Cancer Research, University of Wisconsin, Madison 53706.

The beta 2-tubulin isotype of Physarum polycephalum is only 83% identical in amino acid sequence with the constitutively expressed beta 1B-tubulin and the myxamoeba-specific beta 1A-tubulin isotypes. A polyclonal antibody specific for beta 2-tubulin was used to monitor the subcellular distribution of the beta 2-tubulin antigen in the mitotic spindle of the mature plasmodium - the sole microtubular array in that stage of Physarum. By immunofluorescence, the beta 2-tubulin antigen was detected throughout this anastral mitotic spindle, at all stages of mitosis. Physarum myxamoebae contain astral mitotic spindles and cytoskeletal microtubules. No beta 2-tubulin antigen was detected in the myxamoebal stage. However, as cultures of myxamoebae developed into plasmodia, the beta 2-tubulin antigen was found in the astral mitotic spindles and cytoskeletons in developing cells. Thus, the presence of the plasmodial beta 2-tubulin isotype in a mitotic spindle does not determine a closed, anastral mitosis.




© The Company of Biologists Ltd 1989