spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Koffer, A.
Right arrow Articles by Gomperts, B. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Koffer, A.
Right arrow Articles by Gomperts, B. D.

Journal of Cell Science, Vol 94, Issue 3 585-591, Copyright © 1989 by Company of Biologists

JOURNAL ARTICLES

Soluble proteins as modulators of the exocytotic reaction of permeabilised rat mast cells

A Koffer and BD Gomperts
Department of Physiology, University College London, UK.

This study addresses the question of the role of cytoplasmic proteins in exocytosis from permeabilised rat mast cells. We have used two different methods of cell permeabilisation (ATP4- and streptolysin O) to regulate the size of the plasma membrane lesions, and thus to dictate the rate and extent of efflux of the cytosolic proteins, and compared the secretory response of the two preparations. We report evidence for the existence of two factors present in the cytosol, which affect the exocytotic mechanism in opposing manners. One of these is required for the maintenance of cell responsiveness; it is retained for more than 120 min by ATP4- -permeabilised cells but lost within 60 min from cells permeabilised by streptolysin O. The other factor, which leaks immediately from cells treated from streptolysin O, but only gradually from cells treated with ATP4-, has the effect of suppressing the affinity for both Ca2+ and guanine nucleotide in the exocytotic reaction.


This article has been cited by other articles:


Home page
 Mol. Biol. CellHome page
A. M. Brown, A. J. O'Sullivan, and B. D Gomperts
Induction of Exocytosis from Permeabilized Mast Cells by the Guanosine Triphosphatases Rac and Cdc42
Mol. Biol. Cell, May 1, 1998; 9(5): 1053 - 1063.
[Abstract] [Full Text]

Home page
 J. Cell Sci.Home page
Y. Borovikov, J. Norman, L. Price, A Weeds, and A Koffer
Secretion from permeabilised mast cells is enhanced by addition of gelsolin: contrasting effects of endogenous gelsolin
J. Cell Sci., January 2, 1995; 108(2): 657 - 666.
[Abstract] [PDF]


© The Company of Biologists Ltd 1989