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Journal of Cell Science, Vol 94, 663-674, Copyright © 1989 by Company of Biologists
Submitted on August 7, 1989
Accepted on September 13, 1989
1 Biology Department, York University, 4700 Keele Street, North York, Ontario M3J 1P3, Canada
2 Biology Department, York University, 4700 Keele Street, North York, Ontario M3J 1P3, Canada; Department of Microbiology, Kangwon University, Chunchon 200, South Korea
Author for correspondence
Nuclei of Pleurotus ostreatus migrate in a highly predictable manner following conjugate mitosis at clamp connections. Parameters were determined by observations in living hyphae and these data were used to predict the living behaviour of freezesubstituted nuclei. Three of four classes of nucleus migrate immediately after telophase and move at similar speeds. Freeze-substitution electron microscopy shows that these nuclei have prominent nucleus-associated organelles (NAOs) with large astral microtubule (MT) arrays. Although the NAOs do not have a consistent position with respect to the nuclear motion, they are preferentially located near the hyphal axis. The fourth class of nucleus remains in the developing clamp until it fuses with the main hypha, whereupon it migrates to its interphase position at a rate much faster than the other classes. This class of nucleus has a small NAO and no astral MTs. Treatment of hyphae with a MTdisrupting drug, MBC, reduced the astral arrays in the first three classes but did not slow their rate of movement. Moreover, serial section analysis of drug-treated nuclei whose migration rate at the time of fixation was known showed no relationship, positive or negative, between astral MT number and rate of movement. These data suggest that astral MTs neither generate nor transduce force for post-mitotic nuclear migration in P. ostreatus. The role of astral MTs and possible mechanisms of postmitotic nuclear migration are discussed.
Key words: actin, cytoskeletal interactions, migrating nuclei, Pleurotus ostreatus
Submitted on August 7, 1989
Accepted on September 13, 1989
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