|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 94, Issue 4 703-711, Copyright © 1989 by Company of Biologists
JOURNAL ARTICLES |
A Draeger, EH Stelzer, M Herzog and JV Small
Institute of Molecular Biology, Austrian Academy of Sciences, Salzburg.
Adherens junctions in isolated avian gizzard smooth muscle cells appear as short longitudinal streaks or chevrons that are arranged in periodic, mainly transverse bands along the cell surface. This barrel-like geometry, revealed by antibodies to either vinculin or talin, was seen also in teased gizzard strips by confocal laser-scanning microscopy and contrasted with the rib-like surface pattern observed here and previously in other avian and mammalian smooth muscles. There were on average 67 transverse bands per gizzard cell and an estimated total of around 800 vinculin/talin sites. The longitudinal spacing between the transverse bands of vinculin streaks in the gizzard cells changed from 4-5 microns in extended cells to around 1 micron in shortened cells and the bands remained essentially transverse at all cell lengths, inconsistent with a screw-like mode of cell shortening as has been invoked for smooth muscle cells by others. The absence of rotation on shortening was confirmed by observations on isolated and bead-decorated skinned cells that were induced to contract with ATP. Counterlabelling of cells with alpha-actinin antibodies produced more or less exclusive staining of the cytoplasmic dense bodies, and little surface label: the total number of dense bodies per cell, estimated from confocal microscope through focal series was in the range of 3000. The data are consistent with a periodic anchorage of actin filaments to the cell surface and, in turn, with the existence of regularly spaced contractile assemblies.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  S. J. Gunst and W. Zhang Actin cytoskeletal dynamics in smooth muscle: a new paradigm for the regulation of smooth muscle contraction Am J Physiol Cell Physiol, September 1, 2008; 295(3): C576 - C587. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Kato, K. Nakamura, H. Kudo, Y. H. Tran, Y. Yamamoto, H. Doi, and S. Hirose Characterization of the Column and Autocellular Junctions That Define the Vasculature of Gill Lamellae J. Histochem. Cytochem., September 1, 2007; 55(9): 941 - 953. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Chang, C. Sutherland, E. Hesse, R. Winkfein, W. B. Wiehler, M. Pho, C. Veillette, S. Li, D. P. Wilson, E. Kiss, et al. Identification of a novel interaction between the Ca2+-binding protein S100A11 and the Ca2+- and phospholipid-binding protein annexin A6 Am J Physiol Cell Physiol, April 1, 2007; 292(4): C1417 - C1430. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Zhang and S. J. Gunst Dynamic association between {alpha}-actinin and {beta}-integrin regulates contraction of canine tracheal smooth muscle J. Physiol., May 1, 2006; 572(3): 659 - 676. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. J. Eddinger, J. D. Schiebout, and D. R. Swartz Smooth muscle adherens junctions associated proteins are stable at the cell periphery during relaxation and activation Am J Physiol Cell Physiol, December 1, 2005; 289(6): C1379 - C1387. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Opazo Saez, W. Zhang, Y. Wu, C. E. Turner, D. D. Tang, and S. J. Gunst Tension development during contractile stimulation of smooth muscle requires recruitment of paxillin and vinculin to the membrane Am J Physiol Cell Physiol, February 1, 2004; 286(2): C433 - C447. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. J. Sampson, M. L. Leyland, and C. Dart Direct Interaction between the Actin-binding Protein Filamin-A and the Inwardly Rectifying Potassium Channel, Kir2.1 J. Biol. Chem., October 24, 2003; 278(43): 41988 - 41997. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. T. Gerthoffer and S. J. Gunst Signal Transduction in Smooth Muscle: Invited Review: Focal adhesion and small heat shock proteins in the regulation of actin remodeling and contractility in smooth muscle J Appl Physiol, August 1, 2001; 91(2): 963 - 972. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Fabry, G. N. Maksym, S. A. Shore, P. E. Moore, R. A. Panettieri Jr., J. P. Butler, and J. J. Fredberg Signal Transduction in Smooth Muscle: Selected Contribution: Time course and heterogeneity of contractile responses in cultured human airway smooth muscle cells J Appl Physiol, August 1, 2001; 91(2): 986 - 994. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Ehler, R. Horowits, C. Zuppinger, R. L. Price, E. Perriard, M. Leu, P. Caroni, M. Sussman, H. M. Eppenberger, and J.-C. Perriard Alterations at the Intercalated Disk Associated with the Absence of Muscle Lim Protein J. Cell Biol., May 14, 2001; 153(4): 763 - 772. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. B. Babiychuk and A. Draeger Annexins in Cell Membrane Dynamics: Ca2+-Regulated Association of Lipid Microdomains J. Cell Biol., September 4, 2000; 150(5): 1113 - 1124. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Mehta, D. D. Tang, M.-F. Wu, S. Atkinson, and S. J. Gunst Role of Rho in Ca2+-insensitive contraction and paxillin tyrosine phosphorylation in smooth muscle Am J Physiol Cell Physiol, August 1, 2000; 279(2): C308 - C318. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. B. Babiychuk, R.-J. T. S. Palstra, J. Schaller, U. Kampfer, and A. Draeger Annexin VI Participates in the Formation of a Reversible, Membrane-Cytoskeleton Complex in Smooth Muscle Cells J. Biol. Chem., December 3, 1999; 274(49): 35191 - 35195. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Meiss Influence of intercellular tissue connections on airway muscle mechanics J Appl Physiol, January 1, 1999; 86(1): 5 - 15. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Tang, D. Mehta, and S. J. Gunst Mechanosensitive tyrosine phosphorylation of paxillin and focal adhesion kinase in tracheal smooth muscle Am J Physiol Cell Physiol, January 1, 1999; 276(1): C250 - C258. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Yano, K.'i. Hayashi, T. Momiyama, H. Saga, M. Haruna, and K. Sobue Transcriptional Regulation of the Chicken Caldesmon Gene J. Biol. Chem., October 6, 1995; 270(40): 23661 - 23666. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Terasaki, H Nakagawa, E Kotani, H Mori, and K Ohashi A high molecular mass protein isolated from chicken gizzard: its localization at the dense plaques and dense bodies of smooth muscle and the Z-disks of skeletal muscle J. Cell Sci., January 3, 1995; 108(3): 857 - 868. [Abstract] [PDF]
|
|
|
|
|
|
A. North, M Gimona, R. Cross, and J. Small Calponin is localised in both the contractile apparatus and the cytoskeleton of smooth muscle cells J. Cell Sci., January 3, 1994; 107(3): 437 - 444. [Abstract] [PDF]
|
|
|
|
|
|
A. Belkin, I. Klimanskaya, M. Lukashev, K Lilley, D. Critchley, and V. Koteliansky A novel phosphoglucomutase-related protein is concentrated in adherens junctions of muscle and nonmuscle cells J. Cell Sci., January 1, 1994; 107(1): 159 - 173. [Abstract] [PDF]
|
|
