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Journal of Cell Science, Vol 94, Issue 4 725-731, Copyright © 1989 by Company of Biologists

JOURNAL ARTICLES

Further characterization of the antigen defined by the monoclonal antibody M27

ME Bramwell and SM Humm
Cell Biology Unit, Sir William Dunn School of Pathology, University of Oxford, England.

Using immunoblotting techniques, the antigen that binds the monoclonal antibody M27 has been clearly defined in terms of apparent molecular mass and distribution. In reducing conditions it has an apparent mass of 178K (K = 10(3) Mr) and is present in the cytoplasm and membranes of all mammalian tissue culture cells so far examined. It is absent from lines derived from avian, piscine and amphibian sources. It is also absent from foetal liver of both rat and mouse, but subsequently appears after cultivation in vitro. Similarly, it can be detected on rat lymphocytes only after mitogenic stimulation. However, it is found on both hepatoma and lymphoma cells in vitro, and on in vivo tumours from murine sources. It thus appears to be associated with cell proliferation.




© The Company of Biologists Ltd 1989