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Journal of Cell Science, Vol 95, Issue 1 177-183, Copyright © 1990 by Company of Biologists

JOURNAL ARTICLES

Normal chemotaxis in Dictyostelium discoideum cells with a depolarized plasma membrane potential

B Van Duijn, SA Vogelzang, DL Ypey, LG Van der Molen and PJ Van Haastert
Cell Biology and Genetics Unit, Leiden University, The Netherlands.

We examined a possible role for the plasma membrane potential in signal transduction during cyclic AMP-induced chemotaxis in the cellular slime mold Dictyostelium discoideum. Chemotaxis, cyclic GMP and cyclic AMP responses in cells with a depolarized membrane potential were measured. Cells can be completely depolarized by two different methods: (1) by treatment with azide; this probably causes inhibition of the electrogenic proton pump, which was shown earlier to regulate plasma membrane potential in D. discoideum. (2) By electroporation, which causes the formation of large non-ion-selective pores in the plasma membrane. It was found that in depolarized cells the cyclic AMP-mediated cyclic AMP accumulation was inhibited. In contrast, chemotaxis to a cyclic AMP source was normal; the cyclic AMP-induced accumulation of cyclic GMP, which is known to mediate the chemotactic response, was also not affected. We conclude that membrane-potential-regulated processes, such as voltage-gated ion channels, do not play an essential role in chemotaxis in D. discoideum.


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© The Company of Biologists Ltd 1990