|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 96, 47-61, Copyright © 1990 by Company of Biologists
Submitted on January 3, 1990
Accepted on February 13, 1990
1 School of Biological Sciences, University of Sydney, NSW 2006, Australia
2 Department of Plant Sciences, South Parks Rd, Oxford University, Oxford 0X1 3PF, England; School of Biological and Molecular Sciences, Oxford Polytechnic, Gipsy Lane, Headington, Oxford 0X3 OBP, England
Author for correspondence
We have developed a useful infiltration and embedding technique for cryosectioning and immunostaining plant tissues, which allows the examination of microtubule (MT) arrays across large areas of subepidermal tissue. Using the wound response as a tool for investigating the processes underlying the establishment of a new cell polarity, we have found that a re-orientation of interphase arrays is initiated between 2 and 5 hours post-wounding. This demonstrates that new cell polarity is established sooner after wounding than was previously thought. This cytoskeletal shift appears to act as a spatial precursor for the subsequent change in growth axis, which is manifested in the cell expansion planes, and positioning of the pre-prophase band (PPB) and new cell walls. We have demonstrated that interphase MT reorientation precedes and predicts new cell polarity in a higher plant tissue. Possible mechanisms important in orchestrating this dramatic developmental change could be electric fields, generated by ionic wound currents, and the alteration of mechanical stress patterns within the wounded tissue.
Key words: microtubules, polarity, wounding, root, higher plant
Submitted on January 3, 1990
Accepted on February 13, 1990
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  O. Hamant, M. G. Heisler, H. Jonsson, P. Krupinski, M. Uyttewaal, P. Bokov, F. Corson, P. Sahlin, A. Boudaoud, E. M. Meyerowitz, et al. Developmental Patterning by Mechanical Signals in Arabidopsis Science, December 12, 2008; 322(5908): 1650 - 1655. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Bannigan, A. M. D. Wiedemeier, R. E. Williamson, R. L. Overall, and T. I. Baskin Cortical Microtubule Arrays Lose Uniform Alignment Between Cells and are Oryzalin Resistant in the Arabidopsis Mutant, radially swollen 6 Plant Cell Physiol., July 1, 2006; 47(7): 949 - 958. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. D. X. Chuong, A. G. Good, G. J. Taylor, M. C. Freeman, G. B. G. Moorhead, and D. G. Muench Large-scale Identification of Tubulin-binding Proteins Provides Insight on Subcellular Trafficking, Metabolic Channeling, and Signaling in Plant Cells Mol. Cell. Proteomics, October 1, 2004; 3(10): 970 - 983. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Dhonukshe, A. M. Laxalt, J. Goedhart, T. W. J. Gadella, and T. Munnik Phospholipase D Activation Correlates with Microtubule Reorganization in Living Plant Cells PLANT CELL, November 1, 2003; 15(11): 2666 - 2679. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Gardiner, D. A. Collings, J. D. I. Harper, and J. Marc The Effects of the Phospholipase D-Antagonist 1-Butanol on Seedling Development and Microtubule Organisation in Arabidopsis Plant Cell Physiol., July 15, 2003; 44(7): 687 - 696. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Inoue, S.-y. Yamada, Y. Nagata, and T. Shimmen Rhizoid Differentiation in Spirogyra: Position Sensing by Terminal Cells Plant Cell Physiol., May 15, 2002; 43(5): 479 - 483. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Gardiner, J. D. I. Harper, N. D. Weerakoon, D. A. Collings, S. Ritchie, S. Gilroy, R. J. Cyr, and J. Marc A 90-kD Phospholipase D from Tobacco Binds to Microtubules and the Plasma Membrane PLANT CELL, September 1, 2001; 13(9): 2143 - 2158. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. , B. E. , R. , B. , and A. The cytoplast concept in dividing plant cells: cytoplasmic domains and the evolution of spatially organized cell Am. J. Botany, February 1, 1999; 86(2): 153 - 172. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. L. Cleary and L. G. Smith The Tangled1 Gene Is Required for Spatial Control of Cytoskeletal Arrays Associated with Cell Division during Maize Leaf Development PLANT CELL, November 1, 1998; 10(11): 1875 - 1888. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Hush, P Wadsworth, D. Callaham, and P. Hepler Quantification of microtubule dynamics in living plant cells using fluorescence redistribution after photobleaching J. Cell Sci., January 4, 1994; 107(4): 775 - 784. [Abstract] [PDF]
|
|
|
|
|
|
A. L. Cleary, B. Gunning, G. O. Wasteneys, and P. K. Hepler Microtubule and F-actin dynamics at the division site in living Tradescantia stamen hair cells J. Cell Sci., December 1, 1992; 103(4): 977 - 988. [Abstract] [PDF]
|
|
