spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Keller, H. U.
Right arrow Articles by Portmann, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Keller, H. U.
Right arrow Articles by Portmann, R.

Journal of Cell Science, Vol 96, Issue 1 99-106, Copyright © 1990 by Company of Biologists

JOURNAL ARTICLES

The protein kinase C inhibitor H-7 activates human neutrophils: effect on shape, actin polymerization, fluid pinocytosis and locomotion

HU Keller, V Niggli, A Zimmermann and R Portmann
Institute of Pathology, University of Bern, Switzerland.

The present study demonstrates new properties of H-7. The protein kinase inhibitor H-7 is a potent activator of several neutrophil functions. Stimulation of initially spherical nonmotile neutrophils elicits vigorous shape changes within a few seconds, increases in cytoskeletal actin, altered F-actin distribution, increased adhesiveness and a relatively small increase in pinocytic activity. H-7 has also chemokinetic activities. Depending on the experimental condition, H-7 may elicit or inhibit neutrophil locomotion. It failed to induce chemotaxis. Thus, the response pattern elicited by H-7 is different from that of other leukocyte activators such as chemotactic peptides, PMA or diacylglycerols. The finding that H-7 can elicit shape changes, actin polymerization and pinocytosis suggests that these events can occur without activation of protein kinase C (PKC). PMA-induced shape changes and stimulation of pinocytosis were not inhibited by H-7.


This article has been cited by other articles:


Home page
 Arch OphthalmolHome page
I. Sabanay, B'A. T. Gabelt, B. Tian, P. L. Kaufman, and B. Geiger
H-7 Effects on the Structure and Fluid Conductance of Monkey Trabecular Meshwork
Arch Ophthalmol, July 1, 2000; 118(7): 955 - 962.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
T. A. Wyatt, H. Ito, T. J. Veys, and J. R. Spurzem
Stimulation of protein kinase C activity by tumor necrosis factor-alpha in bovine bronchial epithelial cells
Am J Physiol Lung Cell Mol Physiol, November 1, 1997; 273(5): L1007 - L1012.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
P. Bennett, R. Dixon, and S Kellie
The phosphotyrosine phosphatase inhibitor vanadyl hydroperoxide induces morphological alterations, cytoskeletal rearrangements and increased adhesiveness in rat neutrophil leucocytes
J. Cell Sci., January 11, 1993; 106(3): 891 - 901.
[Abstract] [PDF]

Home page
 J. Cell Sci.Home page
A Volz
Regulation of CD18 expression in human neutrophils as related to shape changes
J. Cell Sci., January 10, 1993; 106(2): 493 - 501.
[Abstract] [PDF]


© The Company of Biologists Ltd 1990