|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 96, Issue 2 207-217, Copyright © 1990 by Company of Biologists
JOURNAL ARTICLES |
A Sonnenberg, CJ Linders, JH Daams and SJ Kennel
Department of Immunohaematology, Central Laboratory of The Netherlands Red Cross Blood Transfusion Service, Amsterdam.
A member of the integrin family, the alpha 6 beta 4 complex was previously identified on human and mouse carcinoma cell lines by using a rat monoclonal antibody to alpha 6. Here we describe two monoclonal antibodies that recognize epitopes on the beta 4 subunit of the human and mouse alpha 6 beta 4 complexes. The monoclonal antibodies against beta 4 were able to preclear alpha 6 beta 4, but not alpha 6 beta 1 from cell line extracts. A substantial fraction of the total beta 4 subunits present on the cell surface was not associated with alpha 6, as it could not be removed by anti-alpha 6 antibodies, but remained precipitable with anti-beta 4 antibodies. There was no evidence for novel alpha subunits associated with beta 4. The alpha 6 subunit consists of disulfide-linked heavy and light chains. The variability in size of these two chains from different cell types is largely due to differences in modifications of N-linked glycans. Additional heterogeneity may be caused by differential proteolytic cleavage of the alpha 6 precursor. Immunoperoxidase staining of tissue sections of neonatal and adult mice revealed that beta 4 expression is limited to epithelial tissues and peripheral nerves. The alpha 6 subunit has a wider distribution that includes all tissues and cells stained by antibodies against beta 4. Cells and tissue that are positive for alpha 6, but negative for beta 4, may express the alpha 6 beta 1 complex.
This article has been cited by other articles:
|
|
 |
|
  M. Gaudreault, F. Vigneault, M.-E. Gingras, S. Leclerc, P. Carrier, L. Germain, and S. L. Guerin Transcriptional Regulation of the Human {alpha}6 Integrin Gene by the Transcription Factor NFI during Corneal Wound Healing Invest. Ophthalmol. Vis. Sci., September 1, 2008; 49(9): 3758 - 3767. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. A. Lawson, L. Xin, R. U. Lukacs, D. Cheng, and O. N. Witte Isolation and functional characterization of murine prostate stem cells PNAS, January 2, 2007; 104(1): 181 - 186. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. K. Hallikas, J. M. Aaltonen, H. von Koskull, L.-A. Lindberg, L. Valmu, N. Kalkkinen, T. Wahlstrom, H. Kataoka, L. Andersson, D. Lindholm, et al. Identification of Antibodies against HAI-1 and Integrin {alpha}6{beta}4 as Immunohistochemical Markers of Human Villous Cytotrophoblast J. Histochem. Cytochem., July 1, 2006; 54(7): 745 - 752. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Hallmann, N. Horn, M. Selg, O. Wendler, F. Pausch, and L. M. Sorokin Expression and Function of Laminins in the Embryonic and Mature Vasculature Physiol Rev, July 1, 2005; 85(3): 979 - 1000. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-J. Leu, N. Chen, C.-C. Chen, V. Todorovic, T. Bai, V. Juric, Y. Liu, G. Yan, S. C.-T. Lam, and L. F. Lau Targeted Mutagenesis of the Angiogenic Protein CCN1 (CYR61): SELECTIVE INACTIVATION OF INTEGRIN {alpha}6{beta}1-HEPARAN SULFATE PROTEOGLYCAN CORECEPTOR-MEDIATED CELLULAR FUNCTIONS J. Biol. Chem., October 15, 2004; 279(42): 44177 - 44187. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. H.M. Litjens, J. Koster, I. Kuikman, S. van Wilpe, J. M. de Pereda, and A. Sonnenberg Specificity of Binding of the Plectin Actin-binding Domain to {beta}4 Integrin Mol. Biol. Cell, October 1, 2003; 14(10): 4039 - 4050. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Ranaldi, G. Perozzi, A. Truong-Tran, P. Zalewski, and C. Murgia Intracellular distribution of labile Zn(II) and zinc transporter expression in kidney and MDCK cells Am J Physiol Renal Physiol, December 1, 2002; 283(6): F1365 - F1375. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Seppala, R. N. Taylor, H. Koistinen, R. Koistinen, and E. Milgrom Glycodelin: A Major Lipocalin Protein of the Reproductive Axis with Diverse Actions in Cell Recognition and Differentiation Endocr. Rev., August 1, 2002; 23(4): 401 - 430. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. L. Davis, F. Buerger, and A. E. Cress Differential Regulation of a Novel Variant of the {alpha}6 Integrin, {alpha}6p Cell Growth Differ., March 1, 2002; 13(3): 107 - 113. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. M. Da Silva, M. P. Velders, J. D. Nieland, J. T. Schiller, B. J. Nickoloff, and W. M. Kast Physical interaction of human papillomavirus virus-like particles with immune cells Int. Immunol., May 1, 2001; 13(5): 633 - 641. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Kortesmaa, P. Yurchenco, and K. Tryggvason Recombinant Laminin-8 (alpha 4beta 1gamma 1). PRODUCTION, PURIFICATION, AND INTERACTIONS WITH INTEGRINS J. Biol. Chem., May 12, 2000; 275(20): 14853 - 14859. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Gambaletta, A. Marchetti, L. Benedetti, A. M. Mercurio, A. Sacchi, and R. Falcioni Cooperative Signaling between alpha 6beta 4 Integrin and ErbB-2 Receptor Is Required to Promote Phosphatidylinositol 3-Kinase-dependent Invasion J. Biol. Chem., March 31, 2000; 275(14): 10604 - 10610. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. M. Witkowski, G.T. Bowden, R. B. Nagle, and A. E. Cress Altered surface expression and increased turnover of the {alpha}6{beta}4 integrin in an undifferentiated carcinoma Carcinogenesis, February 1, 2000; 21(2): 325 - 330. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. T. Clark and J. F. Bertram Molecular regulation of nephron endowment Am J Physiol Renal Physiol, April 1, 1999; 276(4): F485 - F497. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R van der Neut, A. Cachaco, S Thorsteinsdottir, H Janssen, D Prins, J Bulthuis, M van der Valk, J Calafat, and A Sonnenberg Partial rescue of epithelial phenotype in integrin beta4 null mice by a keratin-5 promoter driven human integrin beta4 transgene J. Cell Sci., January 11, 1999; 112(22): 3911 - 3922. [Abstract] [PDF]
|
|
|
|
 |
|
 N Rinaldi, T F E Barth, D Weis, M Schwarz-Eywill, A Pezzutto, M Lukoschek, D Brocai, and B Brado Loss of laminin and of the laminin receptor integrin subunit alpha 6 in situ correlates with cytokine induced down regulation of alpha 6 on fibroblast-like synoviocytes from rheumatoid arthritis Ann Rheum Dis, September 1, 1998; 57(9): 559 - 565. [Abstract] [Full Text]
|
|
|
|
 |
|
 G. A. Rezniczek, J. M. de Pereda, S. Reipert, and G. Wiche Linking Integrin alpha 6beta 4-based Cell Adhesion to the Intermediate Filament Cytoskeleton: Direct Interaction between the beta 4 Subunit and Plectin at Multiple Molecular Sites J. Cell Biol., April 6, 1998; 141(1): 209 - 225. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D Dogic, P Rousselle, and M Aumailley Cell adhesion to laminin 1 or 5 induces isoform-specific clustering of integrins and other focal adhesion components J. Cell Sci., January 3, 1998; 111(6): 793 - 802. [Abstract] [PDF]
|
|
|
|
|
|
S. Biffo, F. Sanvito, S. Costa, L. Preve, R. Pignatelli, L. Spinardi, and P. C. Marchisio Isolation of a Novel beta 4 Integrin-binding Protein (p27BBP) Highly Expressed in Epithelial Cells J. Biol. Chem., November 28, 1997; 272(48): 30314 - 30321. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Ardini, E. Tagliabue, A. Magnifico, S. Buto, V. Castronovo, M. I. Colnaghi, and S. Menard Co-regulation and Physical Association of the 67-kDa Monomeric Laminin Receptor and the alpha 6beta 4 Integrin J. Biol. Chem., January 24, 1997; 272(4): 2342 - 2345. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N Desban and J. Duband Avian neural crest cell migration on laminin: interaction of the alpha1beta1 integrin with distinct laminin-1 domains mediates different adhesive responses J. Cell Sci., January 11, 1997; 110(21): 2729 - 2744. [Abstract] [PDF]
|
|
|
|
|
|
C. Niessen, E. Hulsman, L. Oomen, I Kuikman, and A Sonnenberg A minimal region on the integrin beta4 subunit that is critical to its localization in hemidesmosomes regulates the distribution of HD1/plectin in COS-7 cells J. Cell Sci., January 8, 1997; 110(15): 1705 - 1716. [Abstract] [PDF]
|
|
|
|
|
|
G. O. Delwel, F. Hogervorst, and A. Sonnenberg Cleavage of the alpha6A Subunit Is Essential for Activation of the alpha6Abeta1 Integrin by Phorbol 12-Myristate 13-Acetate J. Biol. Chem., March 29, 1996; 271(13): 7293 - 7296. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M Falk, K Salmivirta, M Durbeej, E Larsson, M Ekblom, D Vestweber, and P Ekblom Integrin alpha 6B beta 1 is involved in kidney tubulogenesis in vitro J. Cell Sci., January 12, 1996; 109(12): 2801 - 2810. [Abstract] [PDF]
|
|
|
|
|
|
C. Niessen, M. van der Raaij-Helmer, E. Hulsman, R van der Neut, M. Jonkman, and A Sonnenberg Deficiency of the integrin beta 4 subunit in junctional epidermolysis bullosa with pyloric atresia: consequences for hemidesmosome formation and adhesion properties J. Cell Sci., January 7, 1996; 109(7): 1695 - 1706. [Abstract] [PDF]
|
|
|
|
|
|
F. Giancotti Signal transduction by the alpha 6 beta 4 integrin: charting the path between laminin binding and nuclear events J. Cell Sci., January 6, 1996; 109(6): 1165 - 1172. [PDF]
|
|
|
|
|
|
F Berdichevsky, D Alford, B D'Souza, and J Taylor-Papadimitriou Branching morphogenesis of human mammary epithelial cells in collagen gels J. Cell Sci., January 12, 1994; 107(12): 3557 - 3568. [Abstract] [PDF]
|
|
|
|
 |
|
 M. Feltri, S. Scherer, R Nemni, J Kamholz, H Vogelbacker, M. Scott, N Canal, V Quaranta, and L Wrabetz Beta 4 integrin expression in myelinating Schwann cells is polarized, developmentally regulated and axonally dependent Development, January 5, 1994; 120(5): 1287 - 1301. [Abstract] [PDF]
|
|
|
|
|
|
P Simon-Assmann, C Leberquier, N Molto, T Uezato, F Bouziges, and M Kedinger Adhesive properties and integrin expression profiles of two colonic cancer populations differing by their spreading on laminin J. Cell Sci., January 3, 1994; 107(3): 577 - 587. [Abstract] [PDF]
|
|
|
|
|
|
C. Schoenenberger, A Zuk, G. Zinkl, D Kendall, and K. Matlin Integrin expression and localization in normal MDCK cells and transformed MDCK cells lacking apical polarity J. Cell Sci., January 2, 1994; 107(2): 527 - 541. [Abstract] [PDF]
|
|
|
|
|
|
C. Niessen, O Cremona, H Daams, S Ferraresi, A Sonnenberg, and P. Marchisio Expression of the integrin alpha 6 beta 4 in peripheral nerves: localization in Schwann and perineural cells and different variants of the beta 4 subunit J. Cell Sci., January 2, 1994; 107(2): 543 - 552. [Abstract] [PDF]
|
|
|
|
|
|
P Laurila and I Leivo Basement membrane and interstitial matrix components form separate matrices in heterokaryons of PYS-2 cells and fibroblasts J. Cell Sci., January 1, 1993; 104(1): 59 - 68. [Abstract] [PDF]
|
|
|
|
|
|
J. Duband, A. Belkin, J Syfrig, J. Thiery, and V. Koteliansky Expression of alpha 1 integrin, a laminin-collagen receptor, during myogenesis and neurogenesis in the avian embryo Development, January 11, 1992; 116(3): 585 - 600. [Abstract] [PDF]
|
|
|
|
|
|
T. L. Davis, I. Rabinovitz, B. W. Futscher, M. Schnolzer, F. Burger, Y. Liu, M. Kulesz-Martin, and A. E. Cress Identification of a Novel Structural Variant of the alpha 6 Integrin J. Biol. Chem., July 6, 2001; 276(28): 26099 - 26106. [Abstract] [Full Text] [PDF]
|
|
