Journal of Cell Science, Vol 96, Issue 3 451-459, Copyright © 1990 by Company of Biologists

JOURNAL ARTICLES

Ribonuclease H(70) is a component of the yeast nuclear scaffold

RM Karwan, T Laroche, U Wintersberger, SM Gasser and M Binder
Institute of Tumorobiology-Cancer Research, University of Vienna, Austria.

We have used monospecific antibodies against three ribonuclease H enzymes of Saccharomyces cerevisiae to investigate their intracellular localization. Fractionation experiments, as well as immunocytochemical staining, revealed a predominantly cytoplasmic localization of the RNase H proteins of 42,000 and 70,000 Mr, whereas that of 55,000 Mr showed equal distribution between nuclei and cytoplasm. The nuclear moiety of ribonuclease H(70) was found to be a part of the yeast nuclear scaffold, as investigated by immunoblotting and antibody inhibition experiments. The 42,000 and 55,000 Mr enzymes, on the other hand, are not scaffold-associated. We conclude that RNase H(70) is part of the nuclear substructure of yeast that was previously found to maintain specific interactions with yeast chromosomal origins of replication (ARS elements).
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