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Journal of Cell Science, Vol 96, Issue 3 501-508, Copyright © 1990 by Company of Biologists

JOURNAL ARTICLES

Adaptation to non-ammoniagenic medium and selective substrate feeding lead to enhanced yields in animal cell cultures

T Hassell and M Butler
Department of Biological Sciences, Manchester Polytechnic, UK.

Methods for the adaptation of three animal cell lines to media in which glutamine is replaced with either glutamate or 2-oxoglutarate are described. The cell lines differ in their rate of adaptation. The consequences of the adaptation of the McCoy cell line to a glutamate-based medium was measured in terms of: cell yield (increased by 17%), ammonia accumulation (reduced by 70%), glucose consumption (decreased by greater than 70%) and lactate accumulation (decreased by greater than 75%). The value of such adaptation and concomitant changes in energy metabolism lies in the potential for increased cell yields. Batch feeding of adapted cells in a microcarrier culture with a cocktail of glucose and amino acids resulted in increases in cell yields of 80% compared to unfed controls.


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R. McDermott and M Butler
Uptake of glutamate, not glutamine synthetase, regulates adaptation of mammalian cells to glutamine-free medium
J. Cell Sci., January 1, 1993; 104(1): 51 - 58.
[Abstract] [PDF]


© The Company of Biologists Ltd 1990