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Journal of Cell Science, Vol 96, Issue 4 591-604, Copyright © 1990 by Company of Biologists
JOURNAL ARTICLES |
E Harfst, NJ Severs and CR Green
Department of Cardiac Medicine, National Heart and Lung Institute, London, England.
It is widely accepted that there is a family of gap junction connexon proteins, their distribution appearing to vary with tissue type and species. In cardiac tissues the major junctional channel component identified is a 43K (K = 10(3) Mr) polypeptide. Using a gap junction isolation protocol in which low temperatures are maintained, and which is detergent-free, we have identified a second gap junction-related protein in cardiac tissues with an apparent relative molecular mass of 70,000. Antibodies raised to three synthetic peptides matching portions of the 43K gap junction protein cDNA sequence cross-react with the 70K protein, but biochemical studies indicate that these proteins are distinct from one another. The structures that contain the 70K protein are susceptible to fragmentation at warm temperatures, and by electron microscopy this can be correlated with loss of 'minidomains' within the junctional plaque. Using a gap junction enriched-fraction prepared from purified isolated adult myocytes we show that both the 43K and 70K gap junction proteins are present in ventricular cardiac myocytes. In such preparations, and those from whole heart, the 70K protein appears to be the major gap junction-related protein present.
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 J. P. Blackburn, N. S. Peters, H.-I. Yeh, S. Rothery, C. R. Green, and N. J. Severs Upregulation of Connexin43 Gap Junctions During Early Stages of Human Coronary Atherosclerosis Arterioscler. Thromb. Vasc. Biol., August 1, 1995; 15(8): 1219 - 1228. [Abstract] [Full Text]
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 D. Becker, W. Evans, C. Green, and A Warner Functional analysis of amino acid sequences in connexin43 involved in intercellular communication through gap junctions J. Cell Sci., January 4, 1995; 108(4): 1455 - 1467. [Abstract] [PDF]
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M Rosendaal, C. Green, A Rahman, and D Morgan Up-regulation of the connexin43+ gap junction network in haemopoietic tissue before the growth of stem cells J. Cell Sci., January 1, 1994; 107(1): 29 - 37. [Abstract] [PDF]
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R. Gourdie, N. Severs, C. Green, S Rothery, P Germroth, and R. Thompson The spatial distribution and relative abundance of gap-junctional connexin40 and connexin43 correlate to functional properties of components of the cardiac atrioventricular conduction system J. Cell Sci., January 8, 1993; 105(4): 985 - 991. [Abstract] [PDF]
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