|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 97, Issue 2 273-281, Copyright © 1990 by Company of Biologists
JOURNAL ARTICLES |
E Shelden and P Wadsworth
Department of Zoology, University of Massachusetts, Amherst 01003.
The pattern and extent of microtubule assembly during spindle elongation has been examined in PtK1 cells by microinjection of biotin-tubulin and immunolocalization of biotin-tubulin-containing microtubules using antibodies to biotin. PtK1 cells were microinjected at 30 degrees C, incubated for various intervals to allow incorporation of biotin-tubulin into microtubules, then lysed, fixed and stained for biotin-tubulin and total tubulin. When mid- to late anaphase cells were examined at short times post-injection, using conventional fluorescence light microscopy, rapid incorporation of biotin-tubulin was detected throughout the interzonal region, between the separating chromosomes, and in the spindle asters. Using confocal fluorescence microscopy, the segments of biotin-labeled microtubules in the interzonal region were found to be continuous with the distal, or plus-ends, of unlabeled microtubules. When teleophase cells were examined, a marked decline in the extent of incorporation was apparent. Quantitative analysis of the total length of labeled polymer in the interzonal region of cells from mid-anaphase through telophase further reveals that the extent of incorporation was maximal during late anaphase, and decreased during telophase. The measured rate of interzonal microtubule growth remained relatively constant during this period. Our results provide direct evidence for plus-end elongation of interzonal microtubules during spindle elongation and further reveal that interzonal microtubules are highly dynamic during late anaphase spindle elongation. The implications of these results for the mechanism of anaphase B are discussed.
This article has been cited by other articles:
|
|
 |
|
  T. M. Durcan, E. S. Halpin, T. Rao, N. S. Collins, E. K. Tribble, J. E. Hornick, and E. H. Hinchcliffe Tektin 2 is required for central spindle microtubule organization and the completion of cytokinesis J. Cell Biol., June 10, 2008; 181(4): 595 - 603. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. L. Pereira, A. J. Pereira, A. R.R. Maia, K. Drabek, C. L. Sayas, P. J. Hergert, M. Lince-Faria, I. Matos, C. Duque, T. Stepanova, et al. Mammalian CLASP1 and CLASP2 Cooperate to Ensure Mitotic Fidelity by Regulating Spindle and Kinetochore Function Mol. Biol. Cell, October 1, 2006; 17(10): 4526 - 4542. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Sasabe, T. Soyano, Y. Takahashi, S. Sonobe, H. Igarashi, T. J. Itoh, M. Hidaka, and Y. Machida Phosphorylation of NtMAP65-1 by a MAP kinase down-regulates its activity of microtubule bundling and stimulates progression of cytokinesis of tobacco cells Genes & Dev., April 15, 2006; 20(8): 1004 - 1014. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Barbosa, M. Gatt, E. Rebollo, C. Gonzalez, and D. M. Glover Drosophila dd4 mutants reveal that {gamma}TuRC is required to maintain juxtaposed half spindles in spermatocytes J. Cell Sci., March 1, 2003; 116(5): 929 - 941. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. L. Rogers, G. C. Rogers, D. J. Sharp, and R. D. Vale Drosophila EB1 is important for proper assembly, dynamics, and positioning of the mitotic spindle J. Cell Biol., September 3, 2002; 158(5): 873 - 884. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Yang and H. Ma Male Meiotic Spindle Lengths in Normal and Mutant Arabidopsis Cells Plant Physiology, June 1, 2001; 126(2): 622 - 630. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Aist, H Liang, and M. Berns Astral and spindle forces in PtK2 cells during anaphase B: a laser microbeam study J. Cell Sci., January 4, 1993; 104(4): 1207 - 1216. [Abstract] [PDF]
|
|
