|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 97, Issue 3 509-516, Copyright © 1990 by Company of Biologists
JOURNAL ARTICLES |
S Sazer and SW Sherwood
Department of Biochemistry, University of Oxford, UK.
Cell growth and division require the doubling of cellular constituents followed by their equal distribution to the two daughter cells. Within a growing population, the ratio of mitochondrial to cellular volume is maintained, as is the number of mitochondrial genomes per cell. The mechanisms responsible for coordinating nuclear and mitochondrial DNA synthesis, and for balancing increases in cell and mitochondrial size are not well understood. In studies of the fission yeast Schizosaccharomyces pombe we quantified cellular and mitochondrial DNA content by both Southern blot analysis and flow cytometry of cells stained with a variety of DNA-binding fluorochromes, which we show are able to detect nuclear and mitochondrial DNA with different efficiencies. In the conditional cell division cycle mutant cdc10, which is unable to initiate nuclear DNA synthesis, we found that there was an increase in the mitochondrial DNA content in the absence of nuclear DNA replication. This demonstrates that mitochondrial and nuclear DNA synthesis are not obligately linked. We also show that mitochondrial DNA replication is not required for the increase in mitochondrial size that occurs as cells elongate, although this results in a decrease in the ratio of mitochondrial DNA to mitochondrial volume.
This article has been cited by other articles:
|
|
 |
|
  C. R. Houchens, A. Perreault, F. Bachand, and T. J. Kelly Schizosaccharomyces pombe Noc3 Is Essential for Ribosome Biogenesis and Cell Division but Not DNA Replication Eukaryot. Cell, September 1, 2008; 7(9): 1433 - 1440. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Perez-Hidalgo, A. E. Rozalen, C. Martin-Castellanos, and S. Moreno Slk1 is a meiosis-specific Sid2-related kinase that coordinates meiotic nuclear division with growth of the forespore membrane J. Cell Sci., May 1, 2008; 121(9): 1383 - 1392. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Dischinger, A. Krapp, L. Xie, J. R. Paulson, and V. Simanis Chemical genetic analysis of the regulatory role of Cdc2p in the S. pombe septation initiation network J. Cell Sci., March 15, 2008; 121(6): 843 - 853. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Froget, J. Blaisonneau, S. Lambert, and G. Baldacci Cleavage of Stalled Forks by Fission Yeast Mus81/Eme1 in Absence of DNA Replication Checkpoint Mol. Biol. Cell, February 1, 2008; 19(2): 445 - 456. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Chen, C. R.M. Wilkinson, S. Watt, C. J. Penkett, W. M. Toone, N. Jones, and J. Bahler Multiple Pathways Differentially Regulate Global Oxidative Stress Responses in Fission Yeast Mol. Biol. Cell, January 1, 2008; 19(1): 308 - 317. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Fersht, D. Hermand, J. Hayles, and P. Nurse Cdc18/CDC6 activates the Rad3-dependent checkpoint in the fission yeast Nucleic Acids Res., August 13, 2007; 35(16): 5323 - 5337. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. V. Menshikova, V. B. Ritov, R. E. Ferrell, K. Azuma, B. H. Goodpaster, and D. E. Kelley Characteristics of skeletal muscle mitochondrial biogenesis induced by moderate-intensity exercise and weight loss in obesity J Appl Physiol, July 1, 2007; 103(1): 21 - 27. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Jimenez, B. R. Cossio, C. J. Rivard, T. Berl, and J. M. Capasso Cell division in the unicellular microalga Dunaliella viridis depends on phosphorylation of extracellular signal-regulated kinases (ERKs) J. Exp. Bot., March 1, 2007; 58(5): 1001 - 1011. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Krapp, P. Collin, A. Cokoja, S. Dischinger, E. Cano, and V. Simanis The Schizosaccharomyces pombe septation initiation network (SIN) is required for spore formation in meiosis J. Cell Sci., July 15, 2006; 119(14): 2882 - 2891. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E.C. Spikings, J. Alderson, and J.C.St. John Transmission of mitochondrial DNA following assisted reproduction and nuclear transfer Hum. Reprod. Update, July 1, 2006; 12(4): 401 - 415. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. L. Turnbull, I. V. Martin, and P. A. Fantes Activity of Cdc2 and its interaction with the cyclin Cdc13 depend on the molecular chaperone Cdc37 in Schizosaccharomyces pombe J. Cell Sci., January 15, 2006; 119(2): 292 - 302. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Seiple, P. Jaruga, M. Dizdaroglu, and J. T. Stivers Linking uracil base excision repair and 5-fluorouracil toxicity in yeast Nucleic Acids Res., January 10, 2006; 34(1): 140 - 151. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Miyabe, T. Morishita, T. Hishida, S. Yonei, and H. Shinagawa Rhp51-Dependent Recombination Intermediates That Do Not Generate Checkpoint Signal Are Accumulated in Schizosaccharomyces pombe rad60 and smc5/6 Mutants after Release from Replication Arrest Mol. Cell. Biol., January 1, 2006; 26(1): 343 - 353. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Wang, Y. Xu, Y. Han, S. Bao, J. Du, M. Yuan, Z. Xu, and K. Chong Overexpression of RAN1 in Rice and Arabidopsis Alters Primordial Meristem, Mitotic Progress, and Sensitivity to Auxin Plant Physiology, January 1, 2006; 140(1): 91 - 101. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Morishita, F. Furukawa, C. Sakaguchi, T. Toda, A. M. Carr, H. Iwasaki, and H. Shinagawa Role of the Schizosaccharomyces pombe F-Box DNA Helicase in Processing Recombination Intermediates Mol. Cell. Biol., September 15, 2005; 25(18): 8074 - 8083. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Kim, K. Robertson, K. J. L. Mylonas, F. C. Gray, I. Charapitsa, and S. A. MacNeill Contrasting effects of Elg1-RFC and Ctf18-RFC inactivation in the absence of fully functional RFC in fission yeast Nucleic Acids Res., July 21, 2005; 33(13): 4078 - 4089. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. Alonso-Nunez, H. An, A. B. Martin-Cuadrado, S. Mehta, C. Petit, M. Sipiczki, F. del Rey, K. L. Gould, and C. R. Vazquez de Aldana Ace2p Controls the Expression of Genes Required for Cell Separation in Schizosaccharomyces pombe Mol. Biol. Cell, April 1, 2005; 16(4): 2003 - 2017. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kjaerulff, I. Lautrup-Larsen, S. Truelsen, M. Pedersen, and O. Nielsen Constitutive Activation of the Fission Yeast Pheromone-Responsive Pathway Induces Ectopic Meiosis and Reveals Ste11 as a Mitogen-Activated Protein Kinase Target Mol. Cell. Biol., March 1, 2005; 25(5): 2045 - 2059. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Meister, A. Taddei, L. Vernis, M. Poidevin, S. M. Gasser, and G. Baldacci Temporal separation of replication and recombination requires the intra-S checkpoint J. Cell Biol., February 14, 2005; 168(4): 537 - 544. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. G. Cortes, R. Katoh-Fukui, K. Moto, J. C. Ribas, and J. Ishiguro Schizosaccharomyces pombe Pmr1p Is Essential for Cell Wall Integrity and Is Required for Polarized Cell Growth and Cytokinesis Eukaryot. Cell, October 1, 2004; 3(5): 1124 - 1135. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Uchiyama and T. S.-F. Wang The B-Subunit of DNA Polymerase {alpha}-Primase Associates with the Origin Recognition Complex for Initiation of DNA Replication Mol. Cell. Biol., September 1, 2004; 24(17): 7419 - 7434. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Oltra, F. Verde, R. Werner, and G. D'Urso A novel RING-finger-like protein Ini1 is essential for cell cycle progression in fission yeast J. Cell Sci., February 22, 2004; 117(6): 967 - 974. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Balzan, K. Sapienza, D. R. Galea, N. Vassallo, H. Frey, and W. H. Bannister Aspirin commits yeast cells to apoptosis depending on carbon source Microbiology, January 1, 2004; 150(1): 109 - 115. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Hodson, J. M. Bailis, and S. L. Forsburg Efficient labeling of fission yeast Schizosaccharomyces pombe with thymidine and BUdR Nucleic Acids Res., November 1, 2003; 31(21): e134 - e134. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. A. Nilssen, M. Synnes, N. Kleckner, B. Grallert, and E. Boye Intra-G1 arrest in response to UV irradiation in fission yeast PNAS, September 16, 2003; 100(19): 10758 - 10763. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Feng, L. Rodriguez-Menocal, G. Tolun, and G. D'Urso Schizosacchromyces pombe Dpb2 Binds to Origin DNA Early in S Phase and Is Required for Chromosomal DNA Replication Mol. Biol. Cell, August 1, 2003; 14(8): 3427 - 3436. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Perez-Hidalgo, S. Moreno, and P. A. San-Segundo Regulation of meiotic progression by the meiosis-specific checkpoint kinase Mek1 in fission yeast J. Cell Sci., January 15, 2003; 116(2): 259 - 271. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Synnes, E. A. Nilssen, E. Boye, and B. Grallert A novel chk1-dependent G1/M checkpoint in fission yeast J. Cell Sci., September 15, 2002; 115(18): 3609 - 3618. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. A. Tinkelenberg, M. J. Hansbury, and R. D. Ladner dUTPase and Uracil-DNA Glycosylase Are Central Modulators of Antifolate Toxicity in Saccharomyces cerevisiae Cancer Res., September 1, 2002; 62(17): 4909 - 4915. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. K. Chattopadhyay, C. W. Tabor, and H. Tabor Absolute requirement of spermidine for growth and cell cycle progression of fission yeast (Schizosaccharomycespombe) PNAS, August 6, 2002; 99(16): 10330 - 10334. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Borgne, H. Murakami, J. Ayte, and P. Nurse The G1/S Cyclin Cig2p during Meiosis in Fission Yeast Mol. Biol. Cell, June 1, 2002; 13(6): 2080 - 2090. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-k. Huang, S. L. Forsburg, U. P. John, M. J. O'Connell, and T. Hunter Isolation and characterization of the Pin1/Ess1p homologue in Schizosaccharomyces pombe J. Cell Sci., March 12, 2002; 114(20): 3779 - 3788. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. F. Cooper and R. Strich Saccharomycescerevisiae C-Type Cyclin Ume3p/Srb11p Is Required for Efficient Induction and Execution of Meiotic Development Eukaryot. Cell, February 1, 2002; 1(1): 66 - 74. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. L. Troxell, M. A. Sweezy, R. R. West, K. D. Reed, B. D. Carson, A. L. Pidoux, W. Z. Cande, and J. R. McIntosh pkl1+and klp2+: Two Kinesins of the Kar3 Subfamily in Fission Yeast Perform Different Functions in Both Mitosis and Meiosis Mol. Biol. Cell, November 1, 2001; 12(11): 3476 - 3488. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Chang, J. L. Morrell, A. Feoktistova, and K. L. Gould Study of Cyclin Proteolysis in Anaphase-Promoting Complex (APC) Mutant Cells Reveals the Requirement for APC Function in the Final Steps of the Fission Yeast Septation Initiation Network Mol. Cell. Biol., October 1, 2001; 21(19): 6681 - 6694. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. T. Liang and S. L. Forsburg Characterization of Schizosaccharomyces pombe mcm7+ and cdc23+ (MCM10) and Interactions With Replication Checkpoints Genetics, October 1, 2001; 159(2): 471 - 486. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Taricani, H. E. Feilotter, C. Weaver, and P. G. Young Expression of hsp16 in response to nucleotide depletion is regulated via the spc1 MAPK pathway in Schizosaccharomyces pombe Nucleic Acids Res., July 15, 2001; 29(14): 3030 - 3040. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Blanco, L. Pelloquin, and S. Moreno Fission yeast mfr1 activates APC and coordinates meiotic nuclear division with sporulation J. Cell Sci., January 6, 2001; 114(11): 2135 - 2143. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. F. Griffiths, V. F. Liu, P. Nurse, and T. S.-F. Wang Role of Fission Yeast Primase Catalytic Subunit in the Replication Checkpoint Mol. Biol. Cell, January 1, 2001; 12(1): 115 - 128. [Abstract] [Full Text]
|
|
|
|
|
|
D. T. Bishop, W. H. McDonald, K. L. Gould, and S. L. Forsburg Isolation of an essential Schizosaccharomyces pombe gene, prp31+, that links splicing and meiosis Nucleic Acids Res., June 1, 2000; 28(11): 2214 - 2220. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. J. Yoo, Y. H. Jin, Y. K. Jang, P. Bjerling, M. Tabish, S. H. Hong, K. Ekwall, and S. D. Park Fission yeast Hrp1, a chromodomain ATPase, is required for proper chromosome segregation and its overexpression interferes with chromatin condensation Nucleic Acids Res., May 1, 2000; 28(9): 2004 - 2011. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Martín-Castellanos, M. A. Blanco, J. M. de Prada, and S. Moreno The puc1 Cyclin Regulates the G1 Phase of the Fission Yeast Cell Cycle in Response to Cell Size Mol. Biol. Cell, February 1, 2000; 11(2): 543 - 554. [Abstract] [Full Text]
|
|
|
|
|
|
A Borgne and P Nurse The Spd1p S phase inhibitor can activate the DNA replication checkpoint pathway in fission yeast J. Cell Sci., January 12, 2000; 113(23): 4341 - 4350. [Abstract] [PDF]
|
|
|
|
|
|
B Grallert, S. Kearsey, M Lenhard, C. Carlson, P Nurse, E Boye, and K Labib A fission yeast general translation factor reveals links between protein synthesis and cell cycle controls J. Cell Sci., January 4, 2000; 113(8): 1447 - 1458. [Abstract] [PDF]
|
|
|
|
|
|
D Griffiths, M Uchiyama, P Nurse, and T. Wang A novel mutant allele of the chromatin-bound fission yeast checkpoint protein Rad17 separates the DNA structure checkpoints J. Cell Sci., January 3, 2000; 113(6): 1075 - 1088. [Abstract] [PDF]
|
|
|
|
|
|
M. J. Muñoz, E. R. Bejarano, R. R. Daga, and J. Jimenez The Identification of Wos2, a p23 Homologue That Interacts With Wee1 and Cdc2 in the Mitotic Control of Fission Yeasts Genetics, December 1, 1999; 153(4): 1561 - 1572. [Abstract] [Full Text]
|
|
|
|
 |
|
 H. Murakami and P. Nurse Meiotic DNA replication checkpoint control in fission yeast Genes & Dev., October 1, 1999; 13(19): 2581 - 2593. [Abstract] [Full Text]
|
|
|
|
|
|
C. R. Chapman, S. T. Evans, A. M. Carr, and T. Enoch Requirement of Sequences outside the Conserved Kinase Domain of Fission Yeast Rad3p for Checkpoint Control Mol. Biol. Cell, October 1, 1999; 10(10): 3223 - 3238. [Abstract] [Full Text]
|
|
|
|
|
|
W. H. McDonald, R. Ohi, N. Smelkova, D. Frendewey, and K. L. Gould Myb-Related Fission Yeast cdc5p Is a Component of a 40S snRNP-Containing Complex and Is Essential for Pre-mRNA Splicing Mol. Cell. Biol., August 1, 1999; 19(8): 5352 - 5362. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. A. Snaith and S. L. Forsburg Rereplication Phenomenon in Fission Yeast Requires MCM Proteins and Other S Phase Genes Genetics, July 1, 1999; 152(3): 839 - 851. [Abstract] [Full Text]
|
|
|
|
|
|
S. M. Burgess, N. Kleckner, and a. B. M. Weiner Somatic pairing of homologs in budding yeast: existence and modulation Genes & Dev., June 15, 1999; 13(12): 1627 - 1641. [Abstract] [Full Text]
|
|
|
|
|
|
S. M. Burgess, M. Ajimura, and N. Kleckner GCN5-dependent histone H3 acetylation and RPD3-dependent histone H4 deacetylation have distinct, opposing effects on IME2 transcription, during meiosis and during vegetative growth, in budding yeast PNAS, June 8, 1999; 96(12): 6835 - 6840. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. D. Berry, A. Feoktistova, M. D. Wright, and K. L. Gould The Schizosaccharomyces pombe dim1+ Gene Interacts with the Anaphase-Promoting Complex or Cyclosome (APC/C) Component lid1+ and Is Required for APC/C Function Mol. Cell. Biol., April 1, 1999; 19(4): 2535 - 2546. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. DeRyckere, C. L. Smith, and G. S. Martin The Role of Nucleotide Binding and Hydrolysis in the Function of the Fission Yeast cdc18+ Gene Product Genetics, April 1, 1999; 151(4): 1445 - 1457. [Abstract] [Full Text]
|
|
|
|
|
|
E. B. Moynihan and T. Enoch Liz1p, a Novel Fission Yeast Membrane Protein, Is Required for Normal Cell Division When Ribonucleotide Reductase Is Inhibited Mol. Biol. Cell, February 1, 1999; 10(2): 245 - 257. [Abstract] [Full Text]
|
|
|
|
|
|
N Beltraminelli, M Murone, and V Simanis The S. pombe zfs1 gene is required to prevent septation if mitotic progression is inhibited J. Cell Sci., January 9, 1999; 112(18): 3103 - 3114. [Abstract] [PDF]
|
|
|
|
|
|
L Cerutti and V Simanis Asymmetry of the spindle pole bodies and spg1p GAP segregation during mitosis in fission yeast J. Cell Sci., January 7, 1999; 112(14): 2313 - 2321. [Abstract] [PDF]
|
|
|
|
|
|
D. Liang, J. Hodson, and S. Forsburg Reduced dosage of a single fission yeast MCM protein causes genetic instability and S phase delay J. Cell Sci., January 2, 1999; 112(4): 559 - 567. [Abstract] [PDF]
|
|
|
|
|
|
C. S. Breeding, J. Hudson, M. K. Balasubramanian, S. M. Hemmingsen, P. G. Young, and K. L. Gould The cdr2+ Gene Encodes a Regulator of G2/M Progression and Cytokinesis in Schizosaccharomyces pombe Mol. Biol. Cell, December 1, 1998; 9(12): 3399 - 3415. [Abstract] [Full Text]
|
|
|
|
|
|
K. Dimitrov and S. Sazer The Role of fnx1, a Fission Yeast Multidrug Resistance Protein, in the Transition of Cells to a Quiescent G0 State Mol. Cell. Biol., September 1, 1998; 18(9): 5239 - 5246. [Abstract] [Full Text]
|
|
|
|
|
|
S. M. Honigberg and R. H. Lee Snf1 Kinase Connects Nutritional Pathways Controlling Meiosis in Saccharomyces cerevisiae Mol. Cell. Biol., August 1, 1998; 18(8): 4548 - 4555. [Abstract] [Full Text]
|
|
|
|
|
|
K. L. Gould, C. G. Burns, A. Feoktistova, C.-P. Hu, S. G. Pasion, and S. L. Forsburg Fission Yeast cdc24+ Encodes a Novel Replication Factor Required for Chromosome Integrity Genetics, July 1, 1998; 149(3): 1221 - 1233. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Sherman, S. G. Pasion, and S. L. Forsburg Multiple Domains of Fission Yeast Cdc19p (MCM2) Are Required for Its Association with the Core MCM Complex Mol. Biol. Cell, July 1, 1998; 9(7): 1833 - 1845. [Abstract] [Full Text]
|
|
