|
|
|
|
|
||
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 97, Issue 4 607-614, Copyright © 1990 by Company of Biologists
JOURNAL ARTICLES |
B Geiger, T Volberg, D Ginsberg, S Bitzur, I Sabanay and RO Hynes
Department of Chemical Immunology, Weizmann Institute of Science, Rehovot, Israel.
We describe here the preparation and application of antibodies directed against a synthetic, 24 amino acid long, peptide corresponding to the conserved cytoplasmic C terminus of N-cadherin. We demonstrate here that the antibodies to the synthetic peptide react extensively with all known members of the cadherin family and, in addition, recognize novel cadherins in a variety of cells and tissues, suggesting that these antibodies indeed exhibit pan-cadherin reactivity. By Western blot screening of chicken tissues at least 4 different immunoreactive bands were resolved, commonly disclosing 2-3 distinct bands within the same tissue. The pan-cadherin antibodies also displayed a broad interspecies cross reactivity, recognizing cadherins in man, bovine, canine, avian, amphibian and teleost cells. This property renders these antibodies excellent reagents for the cloning and identification of novel cadherins. Immunocytochemical labelling with the pan-cadherin antibodies, at the light- and electron-microscope levels, revealed an extensive reactivity with intercellular adherens junctions in cardiac muscle and in various epithelia. We thus propose that the pan-cadherin antibodies may be used as ubiquitous cadherin probes and serve as markers for adherens junctions.
This article has been cited by other articles:
|
|
 |
|
  C. T. Capaldo and I. G. Macara Depletion of E-Cadherin Disrupts Establishment but Not Maintenance of Cell Junctions in Madin-Darby Canine Kidney Epithelial Cells Mol. Biol. Cell, January 1, 2007; 18(1): 189 - 200. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P Sluka, L O'Donnell, J R Bartles, and P G Stanton FSH regulates the formation of adherens junctions and ectoplasmic specialisations between rat Sertoli cells in vitro and in vivo. J. Endocrinol., May 1, 2006; 189(2): 381 - 395. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. D. J. Wrigley, I. Schurov, E. J. Nunn, A. C. L. Martin, E. E. Clarke, S. Ellis, T. P. Bonnert, M. S. Shearman, and D. Beher Functional Overexpression of {gamma}-Secretase Reveals Protease-independent Trafficking Functions and a Critical Role of Lipids for Protease Activity J. Biol. Chem., April 1, 2005; 280(13): 12523 - 12535. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-A. Montero, L. Carvalho, M. Wilsch-Brauninger, B. Kilian, C. Mustafa, and C.-P. Heisenberg Shield formation at the onset of zebrafish gastrulation Development, March 15, 2005; 132(6): 1187 - 1198. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Hinz, P. Pittet, J. Smith-Clerc, C. Chaponnier, and J.-J. Meister Myofibroblast Development Is Characterized by Specific Cell-Cell Adherens Junctions Mol. Biol. Cell, September 1, 2004; 15(9): 4310 - 4320. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Xia, P. Unger, L. Miller, J. Nelson, and I. H. Gelman The Src-suppressed C Kinase Substrate, SSeCKS, Is a Potential Metastasis Inhibitor in Prostate Cancer Cancer Res., July 1, 2001; 61(14): 5644 - 5651. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. J. Mulholland, S. Dedhar, and A. Wayne Vogl Rat Seminiferous Epithelium Contains a Unique Junction (Ectoplasmic Specialization) with Signaling Properties Both of Cell/Cell and Cell/Matrix Junctions Biol Reprod, January 1, 2001; 64(1): 396 - 407. [Abstract] [Full Text]
|
|
|
|
 |
|
 H. Reinecke, G. H. MacDonald, S. D. Hauschka, and C. E. Murry Electromechanical Coupling between Skeletal and Cardiac Muscle: Implications for Infarct Repair J. Cell Biol., May 1, 2000; 149(3): 731 - 740. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. E. Saffitz, K. G. Green, W. J. Kraft, K. B. Schechtman, and K. A. Yamada Effects of diminished expression of connexin43 on gap junction number and size in ventricular myocardium Am J Physiol Heart Circ Physiol, May 1, 2000; 278(5): H1662 - H1670. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. L. Tran, R. B. Nagle, A. E. Cress, and R. L. Heimark N-Cadherin Expression in Human Prostate Carcinoma Cell Lines : An Epithelial-Mesenchymal Transformation Mediating Adhesion withStromal Cells Am. J. Pathol., September 1, 1999; 155(3): 787 - 798. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Hurst IV, P. L. Goldberg, F. L. Minnear, R. L. Heimark, and P. A. Vincent Rearrangement of adherens junctions by transforming growth factor-beta 1: role of contraction Am J Physiol Lung Cell Mol Physiol, April 1, 1999; 276(4): L582 - L595. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Tselepis, M. Chidgey, A. North, and D. Garrod Desmosomal adhesion inhibits invasive behavior PNAS, July 7, 1998; 95(14): 8064 - 8069. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Navarro, L. Ruco, and E. Dejana Differential Localization of VE- and N-Cadherins in Human Endothelial Cells: VE-Cadherin Competes with N-Cadherin for Junctional Localization J. Cell Biol., March 23, 1998; 140(6): 1475 - 1484. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O Ayalon and B Geiger Cyclic changes in the organization of cell adhesions and the associated cytoskeleton, induced by stimulation of tyrosine phosphorylation in bovine aortic endothelial cells J. Cell Sci., January 3, 1997; 110(5): 547 - 556. [Abstract] [PDF]
|
|
|
|
 |
|
 B. D. Angst, L. U.R. Khan, N. J. Severs, K. Whitely, S. Rothery, R. P. Thompson, A. I. Magee, and R. G. Gourdie Dissociated Spatial Patterning of Gap Junctions and Cell Adhesion Junctions During Postnatal Differentiation of Ventricular Myocardium Circ. Res., January 1, 1997; 80(1): 88 - 94. [Abstract] [Full Text]
|
|
|
|
|
|
I. Kitajima, K. Kawahara, N. Hanyu, H. Shin, T. Tokioka, Y. Soejima, J. Tsutsui, M. Ozawa, T. Shimayama, and I. Maruyama Enhanced E-cadherin expression and increased calcium-dependent cell-cell adhesion in human T-cell leukemia virus type I Tax-expressing PC12 cells J. Cell Sci., March 1, 1996; 109(3): 609 - 617. [Abstract] [PDF]
|
|
|
|
|
|
C. Hertig, M Eppenberger-Eberhardt, S Koch, and H. Eppenberger N-cadherin in adult rat cardiomyocytes in culture. I. Functional role of N-cadherin and impairment of cell-cell contact by a truncated N-cadherin mutant J. Cell Sci., January 1, 1996; 109(1): 1 - 10. [Abstract] [PDF]
|
|
|
|
|
|
C. Hertig, S Butz, S Koch, M Eppenberger-Eberhardt, R Kemler, and H. Eppenberger N-cadherin in adult rat cardiomyocytes in culture. II. Spatio-temporal appearance of proteins involved in cell-cell contact and communication. Formation of two distinct N-cadherin/catenin complexes J. Cell Sci., January 1, 1996; 109(1): 11 - 20. [Abstract] [PDF]
|
|
|
|
|
|
M. Maeda, C. S. Taft, E. W. Bush, E. Holder, W. M. Bailey, H. Neville, M. B. Perryman, and R. D. Bies Identification, Tissue-specific Expression, and Subcellular Localization of the 80- and 71-kDa Forms of Myotonic Dystrophy Kinase Protein J. Biol. Chem., September 1, 1995; 270(35): 20246 - 20249. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Breviario, L. Caveda, M. Corada, I. Martin-Padura, P. Navarro, J. Golay, M. Introna, D. Gulino, M. G. Lampugnani, and E. Dejana Functional Properties of Human Vascular Endothelial Cadherin (7B4/Cadherin-5), an Endothelium-Specific Cadherin Arterioscler. Thromb. Vasc. Biol., August 1, 1995; 15(8): 1229 - 1239. [Abstract] [Full Text]
|
|
|
|
|
|
M Rabino, L Trusolino, M Prat, O Cremona, P Savoia, and P. Marchisio A monoclonal antibody identifies a novel GPI-anchored glycoprotein involved in epithelial intercellular adhesion J. Cell Sci., January 6, 1994; 107(6): 1413 - 1428. [Abstract] [PDF]
|
|
|
|
|
|
J Heasman, D Ginsberg, B Geiger, K Goldstone, T Pratt, C Yoshida-Noro, and C Wylie A functional test for maternally inherited cadherin in Xenopus shows its importance in cell adhesion at the blastula stage Development, January 1, 1994; 120(1): 49 - 57. [Abstract] [PDF]
|
|
|
|
|
|
S. Tamm and S Tamm Dynamic control of cell-cell adhesion and membrane-associated actin during food-induced mouth opening in Beroe J. Cell Sci., January 9, 1993; 106(1): 355 - 364. [Abstract] [PDF]
|
|
|
|
|
|
L Leach, P Clark, M. Lampugnani, A. Arroyo, E Dejana, and J. Firth Immunoelectron characterisation of the inter-endothelial junctions of human term placenta J. Cell Sci., January 4, 1993; 104(4): 1073 - 1081. [Abstract] [PDF]
|
|
|
|
|
|
C Schulze and J. Firth Immunohistochemical localization of adherens junction components in blood-brain barrier microvessels of the rat J. Cell Sci., January 3, 1993; 104(3): 773 - 782. [Abstract] [PDF]
|
|
|
|
|
|
B. Geiger, D. Salomon, M. Takeichi, and R. O. Hynes A chimeric N-cadherin/beta 1-integrin receptor which localizes to both cell-cell and cell-matrix adhesions J. Cell Sci., December 1, 1992; 103(4): 943 - 951. [Abstract] [PDF]
|
|
|
|
|
|
D. B. Stewart, A. I. M. Barth, and W. J. Nelson Differential Regulation of Endogenous Cadherin Expression in Madin-Darby Canine Kidney Cells by Cell-Cell Adhesion and Activation of beta -Catenin Signaling J. Biol. Chem., June 30, 2000; 275(27): 20707 - 20716. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. P. Fadel, M. Szewczenko-Pawlikowski, P. Leclerc, E. Dziak, J. M. Symonds, O. Blaschuk, M. Michalak, and M. Opas Calreticulin Affects beta -Catenin-associated Pathways J. Biol. Chem., July 13, 2001; 276(29): 27083 - 27089. [Abstract] [Full Text] [PDF]
|
|
