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Journal of Cell Science, Vol 98, Issue 2 225-232, Copyright © 1991 by Company of Biologists
JOURNAL ARTICLES |
LJ Nicholson and FM Watt
Keratinocyte Laboratory, Imperial Cancer Research Fund, London, UK.
We have examined the expression of fibronectin and the alpha 5 beta 1 fibronectin receptor during terminal differentiation of human epidermal keratinocytes, using involucrin as a terminal differentiation marker. The levels of mRNAs encoding fibronectin and the alpha 5 and beta 1 integrin subunits were measured in keratinocyte populations that had been enriched for involucrin-negative or -positive cells by unit gravity sedimentation or suspension-induced terminal differentiation. All three mRNAs decreased in abundance during terminal differentiation, and the corresponding proteins were localised by immunofluorescence to the basal layer in stratified colonies. We also examined expression in ndk, a strain of epidermal cells with a complete block in terminal differentiation, which, as a result, do not express involucrin. Messenger RNA levels for fibronectin and the alpha 5 and beta 1 subunits were higher in ndk, than in unfractionated keratinocytes and the corresponding proteins were expressed by all ndk, consistent with a basal keratinocyte phenotype. We conclude that expression of fibronectin and the alpha 5 beta 1 fibronectin receptor decreases during terminal differentiation and that such changes are likely to play a role in the selective migration of terminally differentiating cells from the basal epidermal layer.
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N. Hotchin, N. Kovach, and F. Watt Functional down-regulation of alpha 5 beta 1 integrin in keratinocytes is reversible but commitment to terminal differentiation is not J. Cell Sci., January 12, 1993; 106(4): 1131 - 1138. [Abstract] [PDF]
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F. Watt, M. Kubler, N. Hotchin, L. Nicholson, and J. Adams Regulation of keratinocyte terminal differentiation by integrin-extracellular matrix interactions J. Cell Sci., January 9, 1993; 106(1): 175 - 182. [Abstract] [PDF]
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