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Journal of Cell Science, Vol 99, 91-98, Copyright © 1991 by Company of Biologists

Submitted on December 4, 1990
Accepted on January 24, 1991

The Intermediate Filament-Related System of Higher Plant Cells Shares an Epitope with Cytokeratin 8

JOANNE H. E. ROSS 1, AMANDA HUTCHINGS 2, GEOFFREY W. BUTCHER 2, E. BIRGITTE LANE 3, and CLIVE W. LLOYD 1

1 Department of Cell Biology, John Innes Institute, Colney Lane, Norwich NR4 7UH, UK
2 Monoclonal Antibody Unit, AFRC Institute of Animal Physiology and Genetics Research, Babraham Hall, Cambridge CB2 4AT, UK
3 ICRF Clare Hall Laboratories, South Mimms, Potter's Bar, Herts EN6 3LD, UK; Cell Structure Research Group, CRC Laboratories, Department of Biochemistry, Medical Sciences Institute, The University, Dundee DD1 4HN, UK

Author for reprints and correspondence

It is now apparent that plants possess intermediate filament (IF) antigens that exist either as cytoplasmic fibrillar bundles (FBs) or in a finer form, co-distributing with the microtubule arrays. In a previous study a monoclonal antibody (AFB) against the fibrillar bundles was shown to recognize representatives of what may be considered as a ‘core’ group of type III IFs. Here a monoclonal antibody is described that extends the range of similarity to cytokeratin. MAC322 stains the cytokeratin network in PtK2 cells, unlike AFB that stained vimentin. Like other anti-EF antibodies successfully used against plants, it stains their microtubule-associated system but is also able to stain FBs, with or without methanol pretreatment. By immunoblotting, MAC322 does not recognize the acidic (type I) cytokeratdns in MCF7 and TR146 epithelial cell lines but recognizes cytokeratin 8. This was confirmed by a positive cross-reaction against recombinant human cytokeratin 8. This type II polypeptide is known to be characteristic of simple epithelia, to occur in the earliest stages of amphibian development, and also in some non-epithelial and de-differentiated cells.

The sharing of a cytokeratin 8 epitope between higher plants and animals reinforces the idea that IF forms evolved before the two kingdoms diverged.

Key words: plant cells, intermediate filaments, cytokeratin 8, monoclonal antibody

Submitted on December 4, 1990
Accepted on January 24, 1991




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© The Company of Biologists Ltd 1991