|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 99, Issue 2 283-296, Copyright © 1991 by Company of Biologists
JOURNAL ARTICLES |
BB Finlay, S Ruschkowski and S Dedhar
Department of Biochemistry, University of British Columbia, Vancouver, Canada.
Salmonella bacteria can enter (invade) eukaryotic cells, and exist as intracellular parasites. Confocal, light immunofluorescence and electron microscopy were used to examine various cytoskeletal components of cultured Madin Darby canine kidney (MDCK) and HeLa epithelial cells after infection with Salmonella typhimurium. These bacteria entered and remained within membrane-bound vacuoles and were surrounded by large (5-10 microns) dense structures composed of various cytoskeletal components. These structures consisted of extensive aggregations of polymerized actin, alpha-actinin and tropomyosin above and beside the invading bacterium in both epithelial cell lines. These structures were evident soon after bacterial addition (maximal at 20 min for HeLa cells, 60 min for MDCK cells), and disappeared later in the infection as the cytoskeletal components returned to a more normal distribution after bacterial internalization. Surprisingly, tubulin also aggregated above internalized Salmonella although bacterial entry or penetration through polarized monolayers was not disrupted by the microtubule-inhibiting agent nocadazole (this treatment actually enhanced tubulin accumulation around these organisms). There were little if any rearrangements in intermediate filaments composed of keratin or vimentin. Large amounts of talin also accumulated above and around invading Salmonella, but there was only a minor accumulation of vinculin around a few organisms. Pretreatment of epithelial cells with the microfilament inhibitor cytochalasin D blocked bacterial internalization but did not prevent accumulation of polymerized actin and alpha-actinin directly beneath uninternalized bacteria, yet prevented accumulation of the other cytoskeletal components. These results suggest that Salmonella bind to the surface and trigger a signal in epithelial cells that causes marked rearrangements in various cytoskeletal components, including recruitment of actin filaments and alpha-actinin, which then generates the force necessary for bacterial uptake.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  W. W. S. Kum, S. Lee, G. A. Grassl, R. Bidshahri, K. Hsu, H. J. Ziltener, and B. B. Finlay Lack of Functional P-Selectin Ligand Exacerbates Salmonella Serovar Typhimurium Infection J. Immunol., May 15, 2009; 182(10): 6550 - 6561. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Korotkova, Y. Yarova-Yarovaya, V. Tchesnokova, N. Yazvenko, M. A. Carl, A. E. Stapleton, and S. L. Moseley Escherichia coli DraE Adhesin-Associated Bacterial Internalization by Epithelial Cells Is Promoted Independently by Decay-Accelerating Factor and Carcinoembryonic Antigen-Related Cell Adhesion Molecule Binding and Does Not Require the DraD Invasin Infect. Immun., September 1, 2008; 76(9): 3869 - 3880. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Wasylnka, M. A. Bakowski, J. Szeto, M. B. Ohlson, W. S. Trimble, S. I. Miller, and J. H. Brumell Role for Myosin II in Regulating Positioning of Salmonella-Containing Vacuoles and Intracellular Replication Infect. Immun., June 1, 2008; 76(6): 2722 - 2735. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. C. Boyle, N. F. Brown, J. H. Brumell, and B. B. Finlay Src homology domain 2 adaptors affect adherence of Salmonella enterica serovar Typhimurium to non-phagocytic cells Microbiology, October 1, 2007; 153(10): 3517 - 3526. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Shi and J. E. Casanova Invasion of Host Cells by Salmonella typhimurium Requires Focal Adhesion Kinase and p130Cas Mol. Biol. Cell, November 1, 2006; 17(11): 4698 - 4708. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Shi, G. Scita, and J. E. Casanova WAVE2 Signaling Mediates Invasion of Polarized Epithelial Cells by Salmonella typhimurium J. Biol. Chem., August 19, 2005; 280(33): 29849 - 29855. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. T. Reinicke, J. L. Hutchinson, A. I. Magee, P. Mastroeni, J. Trowsdale, and A. P. Kelly A Salmonella typhimurium Effector Protein SifA Is Modified by Host Cell Prenylation and S-Acylation Machinery J. Biol. Chem., April 15, 2005; 280(15): 14620 - 14627. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. P. Naranatt, H. H. Krishnan, M. S. Smith, and B. Chandran Kaposi's Sarcoma-Associated Herpesvirus Modulates Microtubule Dynamics via RhoA-GTP-Diaphanous 2 Signaling and Utilizes the Dynein Motors To Deliver Its DNA to the Nucleus J. Virol., January 15, 2005; 79(2): 1191 - 1206. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. T. Yam and J. A. Theriot Repeated Cycles of Rapid Actin Assembly and Disassembly on Epithelial Cell Phagosomes Mol. Biol. Cell, December 1, 2004; 15(12): 5647 - 5658. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Rottem Interaction of Mycoplasmas With Host Cells Physiol Rev, April 1, 2003; 83(2): 417 - 432. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. R. Monteville, J. E. Yoon, and M. E. Konkel Maximal adherence and invasion of INT 407 cells by Campylobacter jejuni requires the CadF outer-membrane protein and microfilament reorganization Microbiology, January 1, 2003; 149(1): 153 - 165. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. Hobert, K. A. Sands, R. J. Mrsny, and J. L. Madara Cdc42 and Rac1 Regulate Late Events in Salmonella typhimurium-induced Interleukin-8 Secretion from Polarized Epithelial Cells J. Biol. Chem., December 20, 2002; 277(52): 51025 - 51032. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Carabeo, S. S. Grieshaber, E. Fischer, and T. Hackstadt Chlamydia trachomatis Induces Remodeling of the Actin Cytoskeleton during Attachment and Entry into HeLa Cells Infect. Immun., July 1, 2002; 70(7): 3793 - 3803. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Wasylnka and M. M. Moore Uptake of Aspergillus fumigatus Conidia by Phagocytic and Nonphagocytic Cells In Vitro: Quantitation Using Strains Expressing Green Fluorescent Protein Infect. Immun., June 1, 2002; 70(6): 3156 - 3163. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Reed, M. E. Hobert, C. E. Kolenda, K. A. Sands, M. Rathman, M. O'Connor, S. Lyons, A. T. Gewirtz, P. J. Sansonetti, and J. L. Madara The Salmonella typhimurium Flagellar Basal Body Protein FliE Is Required for Flagellin Production and to Induce a Proinflammatory Response in Epithelial Cells J. Biol. Chem., April 5, 2002; 277(15): 13346 - 13353. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Sekiya, M. Ohishi, T. Ogino, K. Tamano, C. Sasakawa, and A. Abe Supermolecular structure of the enteropathogenic Escherichia coli type III secretion system and its direct interaction with the EspA-sheath-like structure PNAS, September 13, 2001; (2001) 191378598. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Elliott, D. J. Coleman, M. A. Lane, R. C. May, L. M. Machesky, and D. P. Clark Cryptosporidium parvum Infection Requires Host Cell Actin Polymerization Infect. Immun., September 1, 2001; 69(9): 5940 - 5942. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Feng, M. Dey, A. Abe, and T. Takeda Isogenic Strain of Escherichia coli O157:H7 That Has Lost both Shiga Toxin 1 and 2 Genes Clin. Vaccine Immunol., July 1, 2001; 8(4): 711 - 717. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Czerucka, S. Dahan, B. Mograbi, B. Rossi, and P. Rampal Implication of Mitogen-Activated Protein Kinases in T84 Cell Responses to Enteropathogenic Escherichia coli Infection Infect. Immun., March 1, 2001; 69(3): 1298 - 1305. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Criss, D. Ahlgren, T. Jou, B. McCormick, and J. Casanova The GTPase Rac1 selectively regulates Salmonella invasion at the apical plasma membrane of polarized epithelial cells J. Cell Sci., January 4, 2001; 114(7): 1331 - 1341. [Abstract] [PDF]
|
|
|
|
|
|
J. L. Edwards, J. Q. Shao, K. A. Ault, and M. A. Apicella Neisseria gonorrhoeae Elicits Membrane Ruffling and Cytoskeletal Rearrangements upon Infection of Primary Human Endocervical and Ectocervical Cells Infect. Immun., September 1, 2000; 68(9): 5354 - 5363. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Winner, R. Rosengarten, and C. Citti In Vitro Cell Invasion of Mycoplasma gallisepticum Infect. Immun., July 1, 2000; 68(7): 4238 - 4244. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Elliott and D. P. Clark Cryptosporidium parvum Induces Host Cell Actin Accumulation at the Host-Parasite Interface Infect. Immun., April 1, 2000; 68(4): 2315 - 2322. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.-H. Coconnier, V. Liévin, M. Lorrot, and A. L. Servin Antagonistic Activity of Lactobacillus acidophilus LB against Intracellular Salmonella enterica Serovar Typhimurium Infecting Human Enterocyte-Like Caco-2/TC-7 Cells Appl. Envir. Microbiol., March 1, 2000; 66(3): 1152 - 1157. [Abstract] [Full Text]
|
|
|
|
|
|
S. H. M. Ling, X. H. Wang, L. Xie, T. M. Lim, and K. Y. Leung Use of green fluorescent protein (GFP) to study the invasion pathways of Edwardsiella tarda in in vivo and in vitro fish models Microbiology, January 1, 2000; 146(1): 7 - 19. [Abstract] [Full Text]
|
|
|
|
|
|
D. H. Meyer, J. E. Rose, J. E. Lippmann, and P. M. Fives-Taylor Microtubules Are Associated with Intracellular Movement and Spread of the Periodontopathogen Actinobacillus actinomycetemcomitans Infect. Immun., December 1, 1999; 67(12): 6518 - 6525. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. R. Dorn, W. A. Dunn Jr., and A. Progulske-Fox Invasion of Human Coronary Artery Cells by Periodontal Pathogens Infect. Immun., November 1, 1999; 67(11): 5792 - 5798. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Boudeau, A.-L. Glasser, E. Masseret, B. Joly, and A. Darfeuille-Michaud Invasive Ability of an Escherichia coli Strain Isolated from the Ileal Mucosa of a Patient with Crohn's Disease Infect. Immun., September 1, 1999; 67(9): 4499 - 4509. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Zhou, M. S. Mooseker, and J. E. Galan An invasion-associated Salmonella protein modulates the actin-bundling activity of plastin PNAS, August 31, 1999; 96(18): 10176 - 10181. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Goluszko, R. Selvarangan, V. Popov, T. Pham, J. W. Wen, and J. Singhal Decay-Accelerating Factor and Cytoskeleton Redistribution Pattern in HeLa Cells Infected with Recombinant Escherichia coli Strains Expressing Dr Family of Adhesins Infect. Immun., August 1, 1999; 67(8): 3989 - 3997. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Hu and D. J. Kopecko Campylobacter jejuni 81-176 Associates with Microtubules and Dynein during Invasion of Human Intestinal Cells Infect. Immun., August 1, 1999; 67(8): 4171 - 4182. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Badger, M. F. Stins, and K. Sik Kim Citrobacter freundii Invades and Replicates in Human Brain Microvascular Endothelial Cells Infect. Immun., August 1, 1999; 67(8): 4208 - 4215. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. H. Darwin and V. L. Miller Molecular Basis of the Interaction of Salmonella with the Intestinal Mucosa Clin. Microbiol. Rev., July 1, 1999; 12(3): 405 - 428. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L.-M. Chen, S. Bagrodia, R. A. Cerione, and J. E. Galan Requirement of p21-activated Kinase (PAK) for Salmonella typhimurium-induced Nuclear Responses J. Exp. Med., May 3, 1999; 189(9): 1479 - 1488. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J Mounier, V Laurent, A Hall, P Fort, M. Carlier, P. Sansonetti, and C Egile Rho family GTPases control entry of Shigella flexneri into epithelial cells but not intracellular motility J. Cell Sci., January 7, 1999; 112(13): 2069 - 2080. [Abstract] [PDF]
|
|
|
|
|
|
B. R. Dorn, K.-P. Leung, and A. Progulske-Fox Invasion of Human Oral Epithelial Cells by Prevotella intermedia Infect. Immun., December 1, 1998; 66(12): 6054 - 6057. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Abe, U. Heczko, R. G. Hegele, and B. Brett Finlay Two Enteropathogenic Escherichia coli Type III Secreted Proteins, EspA and EspB, Are Virulence Factors J. Exp. Med., November 16, 1998; 188(10): 1907 - 1916. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Carlson and B. D. Jones Inhibition of Salmonella typhimurium Invasion by Host Cell Expression of Secreted Bacterial Invasion Proteins Infect. Immun., November 1, 1998; 66(11): 5295 - 5300. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. L. Borowsky and R. O. Hynes Layilin, A Novel Talin-binding Transmembrane Protein Homologous with C-type Lectins, is Localized in Membrane Ruffles J. Cell Biol., October 19, 1998; 143(2): 429 - 442. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. L. Wells, E. M. A. van de Westerlo, R. P. Jechorek, H. M. Haines, and S. L. Erlandsen Cytochalasin-Induced Actin Disruption of Polarized Enterocytes Can Augment Internalization of Bacteria Infect. Immun., June 1, 1998; 66(6): 2410 - 2419. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Y. Coxon, J. T. Summersgill, J. A. Ramirez, and R. D. Miller Signal Transduction during Legionella pneumophila Entry into Human Monocytes Infect. Immun., June 1, 1998; 66(6): 2905 - 2913. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. J. Hueck Type III Protein Secretion Systems in Bacterial Pathogens of Animals and Plants Microbiol. Mol. Biol. Rev., June 1, 1998; 62(2): 379 - 433. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Reed, M. A. Clark, T. A. Booth, C. J. Hueck, S. I. Miller, B. H. Hirst, and M. A. Jepson Cell-Contact-Stimulated Formation of Filamentous Appendages by Salmonella typhimurium Does Not Depend on the Type III Secretion System Encoded by Salmonella Pathogenicity Island 1 Infect. Immun., May 1, 1998; 66(5): 2007 - 2017. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. D. Mills, S. R. Ruschkowski, M. A. Stein, and B. B. Finlay Trafficking of Porin-Deficient Salmonella typhimurium Mutants inside HeLa Cells: ompR and envZ Mutants Are Defective for the Formation of Salmonella-Induced Filaments Infect. Immun., April 1, 1998; 66(4): 1806 - 1811. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. H. Hong and V. L. Miller Identification of a Novel Salmonella Invasion Locus Homologous to Shigella ipgDE J. Bacteriol., April 1, 1998; 180(7): 1793 - 1802. [Abstract] [Full Text]
|
|
|
|
|
|
P. F. Yang, M. Song, D. A. Grove, and R. P. Ellen Filamentous Actin Disruption and Diminished Inositol Phosphate Response in Gingival Fibroblasts Caused by Treponema denticola Infect. Immun., February 1, 1998; 66(2): 696 - 702. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. H. Lamarcq and M. J. McFall-Ngai Induction of a Gradual, Reversible Morphogenesis of Its Host's Epithelial Brush Border by Vibrio fischeri Infect. Immun., February 1, 1998; 66(2): 777 - 785. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B D Jones Host responses to pathogenic Salmonella infection. Genes & Dev., March 15, 1997; 11(6): 679 - 687. [PDF]
|
|
|
|
 |
|
 D.H. Meyer, K.P. Mintz, and P.M. Fives-Taylor Models of Invasion of Enteric and Periodontal Pathogens Into Epithelial Cells: A Comparative Analysis Critical Reviews in Oral Biology & Medicine, January 1, 1997; 8(4): 389 - 409. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B.B. Finlay Interactions Between Salmonella Typhimurium, Enteropathogenic Escherichia Coli (EPEC), and Host Epithelial Cells Advances in Dental Research, February 1, 1995; 9(1): 31 - 36. [Abstract] [PDF]
|
|
|
|
|
|
J. Morisaki, J. Heuser, and L. Sibley Invasion of Toxoplasma gondii occurs by active penetration of the host cell J. Cell Sci., January 6, 1995; 108(6): 2457 - 2464. [Abstract] [PDF]
|
|
|
|
|
|
F. Garcia-del Portillo, M. G. Pucciarelli, W. A. Jefferies, and B. B. Finlay Salmonella typhimurium induces selective aggregation and internalization of host cell surface proteins during invasion of epithelial cells J. Cell Sci., July 1, 1994; 107(7): 2005 - 2020. [Abstract] [PDF]
|
|
|
|
 |
|
 T. Stossel On the crawling of animal cells Science, May 21, 1993; 260(5111): 1086 - 1094. [Abstract] [PDF]
|
|
|
|
|
|
O. Steele-Mortimer, L. A. Knodler, S. L. Marcus, M. P. Scheid, B. Goh, C. G. Pfeifer, V. Duronio, and B. B. Finlay Activation of Akt/Protein Kinase B in Epithelial Cells by the Salmonella typhimurium Effector SigD J. Biol. Chem., November 22, 2000; 275(48): 37718 - 37724. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Guzman-Verri, E. Chaves-Olarte, C. von Eichel-Streiber, I. Lopez-Goni, M. Thelestam, S. Arvidson, J.-P. Gorvel, and E. Moreno GTPases of the Rho Subfamily Are Required for Brucella abortus Internalization in Nonprofessional Phagocytes. DIRECT ACTIVATION OF Cdc42 J. Biol. Chem., November 21, 2001; 276(48): 44435 - 44443. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Sekiya, M. Ohishi, T. Ogino, K. Tamano, C. Sasakawa, and A. Abe Supermolecular structure of the enteropathogenic Escherichia coli type III secretion system and its direct interaction with the EspA-sheath-like structure PNAS, September 25, 2001; 98(20): 11638 - 11643. [Abstract] [Full Text] [PDF]
|
|
