|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 99, Issue 3 523-534, Copyright © 1991 by Company of Biologists
JOURNAL ARTICLES |
PR Andreassen, DK Palmer, MH Wener and RL Margolis
Fred Hutchinson Cancer Research Center, Seattle, WA 98104.
We have discovered a novel mitosis-specific human autoantigen that arises at the centromeres of prophase chromosomes, but ultimately participates in formation of an organelle that bisects the cell at late anaphase and during telophase. The organelle, discernible as a three-dimensional disc by confocal microscopy, encompasses the entire midzone diameter, and its distribution survives disassembly of interpolar microtubules by cold temperature treatment and detergent lysis of cells. Cytokinetic furrow contraction proceeds normally in dihydrocytochalasin B (DCB)-treated cells, and antigen distribution in the furrow is unaltered. In DCB, the furrow retracts in early interphase, coincident with loss of normal membrane association with the disc, resulting in the formation of binucleate cells. The midzone disc in both drug-treated and normal cells is present at the correct time and position to play a central role in cytokinesis. By immunocytochemistry, the disc appears to contain myosin but not actin. The position of the disc and the possible presence of myosin suggest that cytokinesis may involve the interaction of the disc organelle with actin in the cell cortex to produce cleavage in mammalian cells.
This article has been cited by other articles:
|
|
 |
|
  T. M. Yamamoto, A. L. Lewellyn, and J. L. Maller Regulation of the Aurora B Chromosome Passenger Protein Complex during Oocyte Maturation in Xenopus laevis Mol. Cell. Biol., June 15, 2008; 28(12): 4196 - 4203. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-K. Hu, M. Coughlin, C. M. Field, and T. J. Mitchison Cell polarization during monopolar cytokinesis J. Cell Biol., April 21, 2008; 181(2): 195 - 202. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Skoufias, R.-L. Indorato, F. Lacroix, A. Panopoulos, and R. L. Margolis Mitosis persists in the absence of Cdk1 activity when proteolysis or protein phosphatase activity is suppressed J. Cell Biol., November 19, 2007; 179(4): 671 - 685. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Zhang, W. Lan, S. C. Ems-McClung, P. T. Stukenberg, and C. E. Walczak Aurora B Phosphorylates Multiple Sites on Mitotic Centromere-associated Kinesin to Spatially and Temporally Regulate Its Function Mol. Biol. Cell, September 1, 2007; 18(9): 3264 - 3276. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-J. Chang, S. Goulding, R. R. Adams, W. C. Earnshaw, and M. Carmena Drosophila Incenp is required for cytokinesis and asymmetric cell division during development of the nervous system J. Cell Sci., March 15, 2006; 119(6): 1144 - 1153. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Mollinari, J.-P. Kleman, Y. Saoudi, S. A. Jablonski, J. Perard, T. J. Yen, and R. L. Margolis Ablation of PRC1 by Small Interfering RNA Demonstrates that Cytokinetic Abscission Requires a Central Spindle Bundle in Mammalian Cells, whereas Completion of Furrowing Does Not Mol. Biol. Cell, March 1, 2005; 16(3): 1043 - 1055. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Scrittori, D. A. Skoufias, F. Hans, V. Gerson, P. Sassone-Corsi, S. Dimitrov, and R. L. Margolis A Small C-Terminal Sequence of Aurora B Is Responsible for Localization and Function Mol. Biol. Cell, January 1, 2005; 16(1): 292 - 305. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Gassmann, A. Carvalho, A. J. Henzing, S. Ruchaud, D. F. Hudson, R. Honda, E. A. Nigg, D. L. Gerloff, and W. C. Earnshaw Borealin: a novel chromosomal passenger required for stability of the bipolar mitotic spindle J. Cell Biol., July 19, 2004; 166(2): 179 - 191. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. B. Alsop and D. Zhang Microtubules continuously dictate distribution of actin filaments and positioning of cell cleavage in grasshopper spermatocytes J. Cell Sci., March 15, 2004; 117(8): 1591 - 1602. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Carvalho, M. Carmena, C. Sambade, W. C. Earnshaw, and S. P. Wheatley Survivin is required for stable checkpoint activation in taxol-treated HeLa cells J. Cell Sci., July 15, 2003; 116(14): 2987 - 2998. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. D. Bishop and J. M. Schumacher Phosphorylation of the Carboxyl Terminus of Inner Centromere Protein (INCENP) by the Aurora B Kinase Stimulates Aurora B Kinase Activity J. Biol. Chem., July 26, 2002; 277(31): 27577 - 27580. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Borel, F. B. Lacroix, and R. L. Margolis Prolonged arrest of mammalian cells at the G1/S boundary results in permanent S phase stasis J. Cell Sci., July 15, 2002; 115(14): 2829 - 2838. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Mollinari, J.-P. Kleman, W. Jiang, G. Schoehn, T. Hunter, and R. L. Margolis PRC1 is a microtubule binding and bundling protein essential to maintain the mitotic spindle midzone J. Cell Biol., June 24, 2002; 157(7): 1175 - 1186. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Eda, S. Yonemura, T. Kato, N. Watanabe, T. Ishizaki, P. Madaule, and S. Narumiya Rho-dependent transfer of Citron-kinase to the cleavage furrow of dividing cells J. Cell Sci., March 11, 2002; 114(18): 3273 - 3284. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. R. Adams, H. Maiato, W. C. Earnshaw, and M. Carmena Essential Roles of Drosophila Inner Centromere Protein (INCENP) and Aurora B in Histone H3 Phosphorylation, Metaphase Chromosome Alignment, Kinetochore Disjunction, and Chromosome Segregation J. Cell Biol., May 14, 2001; 153(4): 865 - 880. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Skoufias, C. Mollinari, F. B. Lacroix, and R. L. Margolis Human Survivin Is a Kinetochore-associated Passenger Protein J. Cell Biol., December 27, 2000; 151(7): 1575 - 1582. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G Manandhar, R. Moreno, C Simerly, K Toshimori, and G Schatten Contractile apparatus of the normal and abortive cytokinetic cells during mouse male meiosis J. Cell Sci., January 12, 2000; 113(23): 4275 - 4286. [Abstract] [PDF]
|
|
|
|
 |
|
 A. Bellissent–Waydelich, M.-T. Vanier, C. Albigès–Rizo, and P. Simon–Assmann Talin Concentrates to the Midbody Region During Mammalian Cell Cytokinesis J. Histochem. Cytochem., November 1, 1999; 47(11): 1357 - 1368. [Abstract] [Full Text]
|
|
|
|
|
|
K. B. Shannon and R. Li The Multiple Roles of Cyk1p in the Assembly and Function of the Actomyosin Ring in Budding Yeast Mol. Biol. Cell, February 1, 1999; 10(2): 283 - 296. [Abstract] [Full Text]
|
|
|
|
|
|
M. S. Savoian, W. C. Earnshaw, A. Khodjakov, and C. L. Rieder Cleavage Furrows Formed between Centrosomes Lacking an Intervening Spindle and Chromosomes Contain Microtubule Bundles, INCENP, and CHO1 but Not CENP-E Mol. Biol. Cell, February 1, 1999; 10(2): 297 - 311. [Abstract] [Full Text]
|
|
|
|
|
|
C. B. O'Connell, S. P. Wheatley, S. Ahmed, and Y.-l. Wang The Small GTP-binding Protein Rho Regulates Cortical Activities in Cultured Cells during Division J. Cell Biol., January 25, 1999; 144(2): 305 - 313. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. P. Wheatley, C. B. O'Connell, and Y.-l. Wang Inhibition of Chromosomal Separation Provides Insights into Cleavage Furrow Stimulation in Cultured Epithelial Cells Mol. Biol. Cell, August 1, 1998; 9(8): 2173 - 2184. [Abstract] [Full Text]
|
|
|
|
 |
|
 R. R. Adams, A. A.M. Tavares, A. Salzberg, H. J. Bellen, and D. M. Glover pavarotti encodes a kinesin-like protein required to organize the central spindle and contractile ring for cytokinesis Genes & Dev., May 15, 1998; 12(10): 1483 - 1494. [Abstract] [Full Text]
|
|
|
|
 |
|
 S. D. Harris and P. R. Kraus Regulation of Septum Formation in Aspergillus nidulans by a DNA Damage Checkpoint Pathway Genetics, March 1, 1998; 148(3): 1055 - 1067. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. G. Giansanti, S. Bonaccorsi, B. Williams, E. V. Williams, C. Santolamazza, M. L. Goldberg, and M. Gatti Cooperative interactions between the central spindle and the contractile ring during Drosophila cytokinesis Genes & Dev., February 1, 1998; 12(3): 396 - 410. [Abstract] [Full Text]
|
|
|
|
|
|
S. P. Wheatley, E. H. Hinchcliffe, M. Glotzer, A. A. Hyman, G. Sluder, and Y.-l. Wang CDK1 Inactivation Regulates Anaphase Spindle Dynamics and Cytokinesis In Vivo J. Cell Biol., July 28, 1997; 138(2): 385 - 393. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Oegema and T. J. Mitchison Rappaport rules: Cleavage furrow induction in animal cells PNAS, May 13, 1997; 94(10): 4817 - 4820. [Full Text] [PDF]
|
|
|
|
|
|
H. Kosako, M. Amano, M. Yanagida, K. Tanabe, Y. Nishi, K. Kaibuchi, and M. Inagaki Phosphorylation of Glial Fibrillary Acidic Protein at the Same Sites by Cleavage Furrow Kinase and Rho-associated Kinase J. Biol. Chem., April 18, 1997; 272(16): 10333 - 10336. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Eckley, A. M. Ainsztein, A. M. Mackay, I. G. Goldberg, and W. C. Earnshaw Chromosomal Proteins and Cytokinesis: Patterns of Cleavage Furrow Formation and Inner Centromere Protein Positioning in Mitotic Heterokaryons and Mid-anaphase Cells J. Cell Biol., March 24, 1997; 136(6): 1169 - 1183. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H Ohkura, T Torok, G Tick, J Hoheisel, I Kiss, and D. Glover Mutation of a gene for a Drosophila kinesin-like protein, Klp38B, leads to failure of cytokinesis J. Cell Sci., January 4, 1997; 110(8): 945 - 954. [Abstract] [PDF]
|
|
|
|
|
|
V Baldin and B Ducommun Subcellular localisation of human wee1 kinase is regulated during the cell cycle J. Cell Sci., January 6, 1995; 108(6): 2425 - 2432. [Abstract] [PDF]
|
|
|
|
|
|
C. Casiano, G Landberg, R. Ochs, and E. Tan Autoantibodies to a novel cell cycle-regulated protein that accumulates in the nuclear matrix during S phase and is localized in the kinetochores and spindle midzone during mitosis J. Cell Sci., January 12, 1993; 106(4): 1045 - 1056. [Abstract] [PDF]
|
|
|
|
|
|
M Jimenez and C Goday A centrosome-associated antibody from Drosophila melanogaster reveals a new microtubule-dependent structure in the equatorial zone of Parascaris univalens embryos J. Cell Sci., January 11, 1993; 106(3): 719 - 730. [Abstract] [PDF]
|
|
|
|
|
|
A. Miller, R. Fluck, J. McLaughlin, and L. Jaffe Calcium buffer injections inhibit cytokinesis in Xenopus eggs J. Cell Sci., January 10, 1993; 106(2): 523 - 534. [Abstract] [PDF]
|
|
|
|
|
|
M. Murata-Hori, M. Tatsuka, and Y.-L. Wang Probing the Dynamics and Functions of Aurora B Kinase in Living Cells during Mitosis and Cytokinesis Mol. Biol. Cell, April 1, 2002; 13(4): 1099 - 1108. [Abstract] [Full Text] [PDF]
|
|
