spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
JCS ePress online publication date 18 Dec 2002
doi: 10.1242/jcs.00255

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jcs.00255v1
116/4/617    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Campbell, L.
Right arrow Articles by Hardwick, K. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Campbell, L.
Right arrow Articles by Hardwick, K. G.

Research Article

Analysis of Bub3 spindle checkpoint function in Xenopus egg extracts


Leigh Campbell and Kevin G. Hardwick*
* Author for correspondence (e-mail: Kevin.Hardwick{at}ed.ac.uk)

The spindle checkpoint delays the onset of anaphase if there are any defects in the interactions between spindle microtubules and kinetochores. This checkpoint has been reconstituted in vitro in Xenopus egg extracts, and here we use antibodies to Xenopus Bub3 (XBub3) to show that this protein is required for both the activation and the maintenance of a spindle checkpoint arrest in egg extracts. We detect two forms of XBub3 in egg extracts and find both to be complexed with the XBub1 and XBubR1 kinases. Only one form of XBub3 is apparent in Xenopus tissue culture (XTC) cells, and localisation studies reveal that, unlike the Mad proteins, which are concentrated at the nuclear periphery, XBub3 is diffusely localised throughout the nucleus during interphase. During early prophase it is recruited to kinetochores, where it remains until chromosomes align at the metaphase plate. We discuss the mechanism by which our {alpha}-XBub3 antibodies interfere with the checkpoint and possible roles for XBub3 in the spindle checkpoint pathway.




This article has been cited by other articles:


Home page
 Mol. Biol. CellHome page
E. Logarinho, T. Resende, C. Torres, and H. Bousbaa
The Human Spindle Assembly Checkpoint Protein Bub3 Is Required for the Establishment of Efficient Kinetochore-Microtubule Attachments
Mol. Biol. Cell, April 1, 2008; 19(4): 1798 - 1813.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
J. Adjaye, R. Herwig, T. C. Brink, D. Herrmann, B. Greber, S. Sudheer, D. Groth, J. W. Carnwath, H. Lehrach, and H. Niemann
Conserved molecular portraits of bovine and human blastocysts as a consequence of the transition from maternal to embryonic control of gene expression
Physiol Genomics, October 19, 2007; 31(2): 315 - 327.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
C. S. Lopes, P. Sampaio, B. Williams, M. Goldberg, and C. E. Sunkel
The Drosophila Bub3 protein is required for the mitotic checkpoint and for normal accumulation of cyclins during G2 and early stages of mitosis
J. Cell Sci., January 1, 2005; 118(1): 187 - 198.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
S. Vigneron, S. Prieto, C. Bernis, J.-C. Labbe, A. Castro, and T. Lorca
Kinetochore Localization of Spindle Checkpoint Proteins: Who Controls Whom?
Mol. Biol. Cell, October 1, 2004; 15(10): 4584 - 4596.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
S. Tournier, Y. Gachet, V. Buck, J. S. Hyams, and J. B.A. Millar
Disruption of Astral Microtubule Contact with the Cell Cortex Activates a Bub1, Bub3, and Mad3-dependent Checkpoint in Fission Yeast
Mol. Biol. Cell, July 1, 2004; 15(7): 3345 - 3356.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. B. Blevins, A. M. Smith, E. M. Phillips, and M. A. Powers
Complex Formation among the RNA Export Proteins Nup98, Rae1/Gle2, and TAP
J. Biol. Chem., May 30, 2003; 278(23): 20979 - 20988.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2002