The fully linked HTML version of this article has now been published.
JCS ePress
online publication date 23 Jan 2003
doi: 10.1242/jcs.00282
Research Article
Non-membranous granular organelle consisting of PCM-1: subcellular distribution and cell-cycle-dependent assembly/disassembly
Akiharu Kubo
and
Shoichiro Tsukita*
* Author for correspondence (e-mail: htsukita{at}mfour.med.kyoto-u.ac.jp)
Centriolar satellites were initially identified as electron-dense spherical granules, 
70-100 nm in diameter, localized around the centrosomes. We have previously identified pericentriolar material 1 (PCM-1), with a molecular mass of 230 kDa, as a component of centriolar satellites. We now show by immunofluorescence microscopy that these granules are not only concentrated around centrioles but also scattered throughout the cytoplasm in various types of mouse cells, leading us tentatively to call them 'PCM-1 granules'. We then found that, when overexpressed, PCM-1 molecules lacking their C-terminal region bound directly with each other through two distinct regions to form large aggregates, which then recruited endogenous PCM-1. These large aggregates as well as endogenous PCM-1 granules appear to be disassembled during mitosis, and re-assembled when the cells entered interphase. These findings suggest that PCM-1 granules are formed by self-aggregation of PCM-1 and that this self-aggregation is regulated in a cell-cycle-dependent manner. Furthermore, we found that PCM-1 granules are distinct from pericentrin-containing granules, and that these two distinct types of granular structures are frequently associated with each other within the cytoplasm. These findings are discussed with special reference to the possible physiological functions of PCM-1 granules.
This article has been cited by other articles:
|
|
|
|
 
J. Kim, S. R. Krishnaswami, and J. G. Gleeson
CEP290 interacts with the centriolar satellite component PCM-1 and is required for Rab8 localization to the primary cilium
Hum. Mol. Genet.,
December 1, 2008;
17(23):
3796 - 3805.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Kamiya, P. L. Tan, K.-i. Kubo, C. Engelhard, K. Ishizuka, A. Kubo, S. Tsukita, A. E. Pulver, K. Nakajima, N. G. Cascella, et al.
Recruitment of PCM1 to the Centrosome by the Cooperative Action of DISC1 and BBS4: A Candidate for Psychiatric Illnesses
Arch Gen Psychiatry,
September 1, 2008;
65(9):
996 - 1006.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Didier, A. Merdes, J.-E. Gairin, and N. Jabrane-Ferrat
Inhibition of Proteasome Activity Impairs Centrosome-dependent Microtubule Nucleation and Organization
Mol. Biol. Cell,
March 1, 2008;
19(3):
1220 - 1229.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. K. Vladar and T. Stearns
Molecular characterization of centriole assembly in ciliated epithelial cells
J. Cell Biol.,
October 3, 2007;
178(1):
31 - 42.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Srsen, N. Gnadt, A. Dammermann, and A. Merdes
Inhibition of centrosome protein assembly leads to p53-dependent exit from the cell cycle
J. Cell Biol.,
August 28, 2006;
174(5):
625 - 630.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Guo, Z. Yang, W. Song, Q. Chen, F. Wang, Q. Zhang, and X. Zhu
Nudel Contributes to Microtubule Anchoring at the Mother Centriole and Is Involved in Both Dynein-dependent and -independent Centrosomal Protein Assembly
Mol. Biol. Cell,
February 1, 2006;
17(2):
680 - 689.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. S. Hames, R. E. Crookes, K. R. Straatman, A. Merdes, M. J. Hayes, A. J. Faragher, and A. M. Fry
Dynamic Recruitment of Nek2 Kinase to the Centrosome Involves Microtubules, PCM-1, and Localized Proteasomal Degradation
Mol. Biol. Cell,
April 1, 2005;
16(4):
1711 - 1724.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Metge, S. Ofori-Acquah, T. Stevens, and R. Balczon
Stat3 Activity Is Required for Centrosome Duplication in Chinese Hamster Ovary Cells
J. Biol. Chem.,
October 1, 2004;
279(40):
41801 - 41806.
[Abstract]
[Full Text]
[PDF]
|
|
|
© The Company of Biologists Ltd 2003
