spacer gif spacer gif spacer gif spacer gif Propose a workshop for 2011 spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
JCS ePress online publication date 12 Feb 2003
doi: 10.1242/jcs.00308

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jcs.00308v1
116/7/1367    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Deroanne, C.
Right arrow Articles by Pouysségur, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Deroanne, C.
Right arrow Articles by Pouysségur, J.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research Article

EphrinA1 inactivates integrin-mediated vascular smooth muscle cell spreading via the Rac/PAK pathway


Christophe Deroanne*, Valérie Vouret-Craviari, Bingcheng Wang, and Jacques Pouysségur
* Author for correspondence (e-mail: c.deroanne{at}ulg.ac.be)

Interactions between the Eph receptor tyrosine kinase and ephrin ligands transduce short-range signals regulating axon pathfinding, development of the cardiovascular system, as well as migration and spreading of neuronal and non-neuronal cells. Some of these effects are believed to be mediated by alterations in actin dynamics. The members of the small Rho GTPase family elicit various effects on actin structures and are probably involved in Eph receptor-induced actin modulation. EphrinA1 is proposed to contribute to angiogenesis as it is strongly expressed at sites of neovascularization. Moreover, angiogenic factors induce the expression of ephrinA1 in endothelial cells. In this study, using rat vascular smooth muscle cells (VSMCs), we investigated the contribution of the small Rho GTPases in ephrinA1-induced integrin inactivation. EphrinA1 did not significantly affect early adhesion of VSMCs on purified laminin or fibronectin, but strongly impaired cell spreading. The Rho kinase inhibitor Y-27632 partly reversed the ephrinA1 effect, suggesting involvement of Rho in this model. However, inhibition of RhoA synthesis with short interfering (si)RNA had a modest effect, suggesting that RhoA plays a limited role in ephrinA1-mediated inhibition of spreading in VSMCs. The ephrinA1-mediated morphological alterations correlated with inhibition of Rac1 and p21-activated kinase 1 (PAK1) activity, and were antagonized by the expression of a constitutively active Rac mutant. Moreover, repression of Rac1 synthesis with siRNA amplifies the ephrinA1-induced inhibition of spreading. Finally, sphingosine-1-phosphate (S1P), a lipid mediator known to inhibit Rac activation in VSMCs amplifies the ephrinA1 effect. In conclusion, our results emphasize the role of the Rac/PAK pathway in ephrinA1-mediated inhibition of spreading. In this way, ephrinA1, alone or in synergy with S1P, can participate in blood vessel destabilization, a prerequisite for angiogenesis.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 Am. J. Pathol.Home page
T. Okazaki, A. Ni, P. Baluk, O. A. Ayeni, J. Kearley, A. J. Coyle, A. Humbles, and D. M. McDonald
Capillary Defects and Exaggerated Inflammatory Response in the Airways of EphA2-Deficient Mice
Am. J. Pathol., June 1, 2009; 174(6): 2388 - 2399.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
M. L. Taddei, M. Parri, A. Angelucci, B. Onnis, F. Bianchini, E. Giannoni, G. Raugei, L. Calorini, N. Rucci, A. Teti, et al.
Kinase-Dependent and -Independent Roles of EphA2 in the Regulation of Prostate Cancer Invasion and Metastasis
Am. J. Pathol., April 1, 2009; 174(4): 1492 - 1503.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
T. Yamazaki, J. Masuda, T. Omori, R. Usui, H. Akiyama, and Y. Maru
EphA1 interacts with integrin-linked kinase and regulates cell morphology and motility
J. Cell Sci., January 15, 2009; 122(2): 243 - 255.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
Y.-X. Qi, M.-J. Qu, D.-K. Long, B. Liu, Q.-P. Yao, S. Chien, and Z.-L. Jiang
Rho-GDP dissociation inhibitor alpha downregulated by low shear stress promotes vascular smooth muscle cell migration and apoptosis: a proteomic analysis
Cardiovasc Res, October 1, 2008; 80(1): 114 - 122.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. T. G. Ho, S. D. Merajver, C. M. Lapiere, B. V. Nusgens, and C. F. Deroanne
RhoA-GDP Regulates RhoB Protein Stability: POTENTIAL INVOLVEMENT OF RhoGDI{alpha}
J. Biol. Chem., August 1, 2008; 283(31): 21588 - 21598.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
D. Arvanitis and A. Davy
Eph/ephrin signaling: networks
Genes & Dev., February 15, 2008; 22(4): 416 - 429.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Shutes, C. Onesto, V. Picard, B. Leblond, F. Schweighoffer, and C. J. Der
Specificity and Mechanism of Action of EHT 1864, a Novel Small Molecule Inhibitor of Rac Family Small GTPases
J. Biol. Chem., December 7, 2007; 282(49): 35666 - 35678.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Parri, F. Buricchi, E. Giannoni, G. Grimaldi, T. Mello, G. Raugei, G. Ramponi, and P. Chiarugi
EphrinA1 Activates a Src/Focal Adhesion Kinase-mediated Motility Response Leading to Rho-dependent Actino/Myosin Contractility
J. Biol. Chem., July 6, 2007; 282(27): 19619 - 19628.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
I. Airoldi, E. Di Carlo, C. Cocco, G. Taverniti, T. D'Antuono, E. Ognio, M. Watanabe, D. Ribatti, and V. Pistoia
From the Cover: Endogenous IL-12 triggers an antiangiogenic program in melanoma cells
PNAS, March 6, 2007; 104(10): 3996 - 4001.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
L. JeBailey, O. Wanono, W. Niu, J. Roessler, A. Rudich, and A. Klip
Ceramide- and Oxidant-Induced Insulin Resistance Involve Loss of Insulin-Dependent Rac-Activation and Actin Remodeling in Muscle Cells
Diabetes, February 1, 2007; 56(2): 394 - 403.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
Y. Goueffic, C. Guilluy, P. Guerin, P. Patra, P. Pacaud, and G. Loirand
Hyaluronan induces vascular smooth muscle cell migration through RHAMM-mediated PI3K-dependent Rac activation
Cardiovasc Res, November 1, 2006; 72(2): 339 - 348.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
M. Philippova, A. Banfi, D. Ivanov, R. Gianni-Barrera, R. Allenspach, P. Erne, and T. Resink
Atypical GPI-Anchored T-Cadherin Stimulates Angiogenesis In Vitro and In Vivo
Arterioscler Thromb Vasc Biol, October 1, 2006; 26(10): 2222 - 2230.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
S. G. Hunter, G. Zhuang, D. Brantley-Sieders, W. Swat, C. W. Cowan, and J. Chen
Essential Role of Vav Family Guanine Nucleotide Exchange Factors in EphA Receptor-Mediated Angiogenesis.
Mol. Cell. Biol., July 1, 2006; 26(13): 4830 - 4842.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
E. Boulter, D. Grall, S. Cagnol, and E. Van Obberghen-Schilling
Regulation of cell-matrix adhesion dynamics and Rac-1 by integrin linked kinase
FASEB J, July 1, 2006; 20(9): 1489 - 1491.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
Y.-J. Wu, M. Bond, G. B. Sala-Newby, and A. C. Newby
Altered S-Phase Kinase-Associated Protein-2 Levels Are a Major Mediator of Cyclic Nucleotide-Induced Inhibition of Vascular Smooth Muscle Cell Proliferation
Circ. Res., May 12, 2006; 98(9): 1141 - 1150.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
F. Tatin, C. Varon, E. Genot, and V. Moreau
A signalling cascade involving PKC, Src and Cdc42 regulates podosome assembly in cultured endothelial cells in response to phorbol ester
J. Cell Sci., February 15, 2006; 119(4): 769 - 781.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
O. M. Pello, B. Duthey, D. Garcia-Bernal, J. M. Rodriguez-Frade, J. V. Stein, J. Teixido, C. Martinez-A., and M. Mellado
Opioids Trigger {alpha}5beta1 Integrin-Mediated Monocyte Adhesion
J. Immunol., February 1, 2006; 176(3): 1675 - 1685.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
T. Ojima, H. Takagi, K. Suzuma, H. Oh, I. Suzuma, H. Ohashi, D. Watanabe, E. Suganami, T. Murakami, M. Kurimoto, et al.
EphrinA1 Inhibits Vascular Endothelial Growth Factor-Induced Intracellular Signaling and Suppresses Retinal Neovascularization and Blood-Retinal Barrier Breakdown
Am. J. Pathol., January 1, 2006; 168(1): 331 - 339.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
D. Garcia-Bernal, N. Wright, E. Sotillo-Mallo, C. Nombela-Arrieta, J. V. Stein, X. R. Bustelo, and J. Teixido
Vav1 and Rac Control Chemokine-promoted T Lymphocyte Adhesion Mediated by the Integrin {alpha}4{beta}1
Mol. Biol. Cell, July 1, 2005; 16(7): 3223 - 3235.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
M. L. Lemons, S. Barua, M. L. Abanto, W. Halfter, and M. L. Condic
Adaptation of Sensory Neurons to Hyalectin and Decorin Proteoglycans
J. Neurosci., May 18, 2005; 25(20): 4964 - 4973.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
O. D. Defawe, R. D. Kenagy, C. Choi, S. Y.C. Wan, C. Deroanne, B. Nusgens, N. Sakalihasan, A. Colige, and A. W. Clowes
MMP-9 regulates both positively and negatively collagen gel contraction: A nonproteolytic function of MMP-9
Cardiovasc Res, May 1, 2005; 66(2): 402 - 409.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. H. Rauch, E. Millette, R. D. Kenagy, G. Daum, J. W. Fischer, and A. W. Clowes
Syndecan-4 Is Required for Thrombin-induced Migration and Proliferation in Human Vascular Smooth Muscle Cells
J. Biol. Chem., April 29, 2005; 280(17): 17507 - 17511.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
D. Bi, J. Nishimura, N. Niiro, K. Hirano, and H. Kanaide
Contractile Properties of the Cultured Vascular Smooth Muscle Cells: The Crucial Role Played by RhoA in the Regulation of Contractility
Circ. Res., April 29, 2005; 96(8): 890 - 897.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
C. F. Deroanne, D. Hamelryckx, T. T. G. Ho, C. A. Lambert, P. Catroux, C. M. Lapiere, and B. V. Nusgens
Cdc42 downregulates MMP-1 expression by inhibiting the ERK1/2 pathway
J. Cell Sci., March 15, 2005; 118(6): 1173 - 1183.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
E. Millette, B. H. Rauch, O. Defawe, R. D. Kenagy, G. Daum, and A. W. Clowes
Platelet-Derived Growth Factor-BB-Induced Human Smooth Muscle Cell Proliferation Depends on Basic FGF Release and FGFR-1 Activation
Circ. Res., February 4, 2005; 96(2): 172 - 179.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
D. Waltregny, L. de Leval, W. Glenisson, S. Ly Tran, B. J. North, A. Bellahcene, U. Weidle, E. Verdin, and V. Castronovo
Expression of Histone Deacetylase 8, a Class I Histone Deacetylase, Is Restricted to Cells Showing Smooth Muscle Differentiation in Normal Human Tissues
Am. J. Pathol., August 1, 2004; 165(2): 553 - 564.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
W. C.Y. Leung, A. Lawrie, S. Demaries, H. Massaeli, A. Burry, S. Yablonsky, J. M. Sarjeant, E. Fera, E. Rassart, J. G. Pickering, et al.
Apolipoprotein D and Platelet-Derived Growth Factor-BB Synergism Mediates Vascular Smooth Muscle Cell Migration
Circ. Res., July 23, 2004; 95(2): 179 - 186.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
D. M. Brantley-Sieders, J. Caughron, D. Hicks, A. Pozzi, J. C. Ruiz, and J. Chen
EphA2 receptor tyrosine kinase regulates endothelial cell migration and vascular assembly through phosphoinositide 3-kinase-mediated Rac1 GTPase activation
J. Cell Sci., April 15, 2004; 117(10): 2037 - 2049.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2003