spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
JCS ePress online publication date 6 May 2003
doi: 10.1242/jcs.00454

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jcs.00454v1
116/12/2551    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gourlay, C. W.
Right arrow Articles by Ayscough, K. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gourlay, C. W.
Right arrow Articles by Ayscough, K. R.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research Article

An interaction between Sla1p and Sla2p plays a role in regulating actin dynamics and endocytosis in budding yeast


Campbell W. Gourlay, Hilary Dewar, Derek T. Warren, Rosaria Costa, Nilima Satish, and Kathryn R. Ayscough*
* Author for correspondence (e-mail: k.ayscough{at}bio.gla.ac.uk)

The importance of a dynamic actin cytoskeleton for facilitating endocytosis has been recognised for many years in budding yeast and is increasingly recognised in mammalian cells. However, the mechanism for actin recruitment and the role it plays in endocytosis is unclear. Here we show the importance of two yeast proteins in this process. We demonstrate that Sla1p and Sla2p interact in vitro and in vivo and that this interaction is mediated by the central domain of Sla2p, which includes its coiled-coil region, and by a domain of Sla1p between residues 118 and 361. Overexpression of the interacting fragment of Sla1p causes reduced fluid-phase endocytosis and, interestingly, defects in subsequent trafficking to vacuoles. We show that Sla2p is required for the polarised localisation of Sla1p in cells but not for its cortical localisation or for its overlapping localisation with actin. Generation of an {Delta}sla1{Delta}sla2 double mutant demonstrates that Sla2p is likely to act upstream of Sla1p in endocytosis, whereas sensitivity to latrunculin-A suggests that the proteins have opposite effects on actin dynamics. We propose that Sla2p recruits Sla1p to endocytic sites. Sla1p and its associated protein Pan1p then regulate actin assembly through interactions with Arp2/3 and Arp2/3-activating proteins Abp1p and Las17/Bee1p.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 Mol. Biol. CellHome page
A. S. Robertson, E. G. Allwood, A. P.C. Smith, F. C. Gardiner, R. Costa, S. J. Winder, and K. R. Ayscough
The WASP Homologue Las17 Activates the Novel Actin-regulatory Activity of Ysc84 to Promote Endocytosis in Yeast
Mol. Biol. Cell, March 15, 2009; 20(6): 1618 - 1628.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
Y. Higuchi, J.-y. Shoji, M. Arioka, and K. Kitamoto
Endocytosis Is Crucial for Cell Polarity and Apical Membrane Recycling in the Filamentous Fungus Aspergillus oryzae
Eukaryot. Cell, January 1, 2009; 8(1): 37 - 46.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
B. Almeida, S. Buttner, S. Ohlmeier, A. Silva, A. Mesquita, B. Sampaio-Marques, N. S. Osorio, A. Kollau, B. Mayer, C. Leao, et al.
NO-mediated apoptosis in yeast
J. Cell Sci., September 15, 2007; 120(18): 3279 - 3288.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
Y. Sun, S. Carroll, M. Kaksonen, J. Y. Toshima, and D. G. Drubin
PtdIns(4,5)P2 turnover is required for multiple stages during clathrin- and actin-dependent endocytic internalization
J. Cell Biol., April 23, 2007; 177(2): 355 - 367.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
T. Takahashi, T. Furuchi, and A. Naganuma
Endocytic Ark/Prk Kinases Play a Critical Role in Adriamycin Resistance in Both Yeast and Mammalian Cells
Cancer Res., December 15, 2006; 66(24): 11932 - 11937.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
E. Smythe and K. R. Ayscough
Actin regulation in endocytosis
J. Cell Sci., November 15, 2006; 119(22): 4589 - 4598.
[Abstract] [Full Text] [PDF]

Home page
 Microbiol. Mol. Biol. Rev.Home page
J. B. Moseley and B. L. Goode
The Yeast Actin Cytoskeleton: from Cellular Function to Biochemical Mechanism
Microbiol. Mol. Biol. Rev., September 1, 2006; 70(3): 605 - 645.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
F. J. Vizeacoumar, W. N. Vreden, M. Fagarasanu, G. A. Eitzen, J. D. Aitchison, and R. A. Rachubinski
The Dynamin-like Protein Vps1p of the Yeast Saccharomyces cerevisiae Associates with Peroxisomes in a Pex19p-dependent Manner
J. Biol. Chem., May 5, 2006; 281(18): 12817 - 12823.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
J. S. Chang, K. Henry, M. I. Geli, and S. K. Lemmon
Cortical Recruitment and Nuclear-Cytoplasmic Shuttling of Scd5p, a Protein Phosphatase-1-targeting Protein Involved in Actin Organization and Endocytosis
Mol. Biol. Cell, January 1, 2006; 17(1): 251 - 262.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
D. Hwang, J. J. Smith, D. M. Leslie, A. D. Weston, A. G. Rust, S. Ramsey, P. de Atauri, A. F. Siegel, H. Bolouri, J. D. Aitchison, et al.
A data integration methodology for systems biology: Experimental verification
PNAS, November 29, 2005; 102(48): 17302 - 17307.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
J. L. D'Agostino and B. L. Goode
Dissection of Arp2/3 Complex Actin Nucleation Mechanism and Distinct Roles for Its Nucleation-Promoting Factors in Saccharomyces cerevisiae
Genetics, September 1, 2005; 171(1): 35 - 47.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
W. Ge, T. G. Chew, V. Wachtler, S. N. Naqvi, and M. K. Balasubramanian
The Novel Fission Yeast Protein Pal1p Interacts with Hip1-related Sla2p/End4p and Is Involved in Cellular Morphogenesis
Mol. Biol. Cell, September 1, 2005; 16(9): 4124 - 4138.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
M. Morishita and J. Engebrecht
End3p-Mediated Endocytosis Is Required for Spore Wall Formation in Saccharomyces cerevisiae
Genetics, August 1, 2005; 170(4): 1561 - 1574.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
S. J. Kavanagh, T. C. Schulz, P. Davey, C. Claudianos, C. Russell, and P. D. Rathjen
A family of RS domain proteins with novel subcellular localization and trafficking
Nucleic Acids Res., March 1, 2005; 33(4): 1309 - 1322.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
A. Gruhler, J. V. Olsen, S. Mohammed, P. Mortensen, N. J. Faergeman, M. Mann, and O. N. Jensen
Quantitative Phosphoproteomics Applied to the Yeast Pheromone Signaling Pathway
Mol. Cell. Proteomics, March 1, 2005; 4(3): 310 - 327.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
Y. Sun, M. Kaksonen, D. T. Madden, R. Schekman, and D. G. Drubin
Interaction of Sla2p's ANTH Domain with PtdIns(4,5)P2 Is Important for Actin-dependent Endocytic Internalization
Mol. Biol. Cell, February 1, 2005; 16(2): 717 - 730.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
A. A. Rodal, L. Kozubowski, B. L. Goode, D. G. Drubin, and J. H. Hartwig
Actin and Septin Ultrastructures at the Budding Yeast Cell Cortex
Mol. Biol. Cell, January 1, 2005; 16(1): 372 - 384.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
X. Yu and M. Cai
The yeast dynamin-related GTPase Vps1p functions in the organization of the actin cytoskeleton via interaction with Sla1p
J. Cell Sci., August 1, 2004; 117(17): 3839 - 3853.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. Fiorani, R. J. D. Reid, A. Schepis, H. R. Jacquiau, H. Guo, P. Thimmaiah, P. Benedetti, and M.-A. Bjornsti
The Deubiquitinating Enzyme Doa4p Protects Cells from DNA Topoisomerase I Poisons
J. Biol. Chem., May 14, 2004; 279(20): 21271 - 21281.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. D. Stamenova, R. Dunn, A. S. Adler, and L. Hicke
The Rsp5 Ubiquitin Ligase Binds to and Ubiquitinates Members of the Yeast CIN85-Endophilin Complex, Sla1-Rvs167
J. Biol. Chem., April 16, 2004; 279(16): 16017 - 16025.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
B. Huang, G. Zeng, A. Y.J. Ng, and M. Cai
Identification of Novel Recognition Motifs and Regulatory Targets for the Yeast Actin-regulating Kinase Prk1p
Mol. Biol. Cell, December 1, 2003; 14(12): 4871 - 4884.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2003