The fully linked HTML version of this article has now been published.
JCS ePress
online publication date 20 May 2003
doi: 10.1242/jcs.00496
Research Article
Fission yeast meu14+ is required for proper nuclear division and accurate forespore membrane formation during meiosis II
Daisuke Okuzaki,
Wataru Satake,
Aiko Hirata,
and
Hiroshi Nojima*
* Author for correspondence (e-mail: hnojima{at}biken.osaka-u.ac.jp)
Using a meiosis-specific subtracted cDNA library of Schizosaccharomyces pombe, we identified meu14+ as a gene whose expression is upregulated during meiosis. Transcription of meu14+ is induced abruptly after the cell enters meiosis. Its transcription is dependent on the meiosis-specific transcription factor Mei4. In meu14
cells, the segregation and modification of the SPBs (spindle pole bodies) and microtubule elongation during meiosis II were aberrant. Meiotic meu14 cells consequently produced a high frequency of abnormal tetranucleate cells harboring aberrant forespore membranes and failed to produce asci. In wild-type cells harboring the integrated meu14+-gfp fusion gene, Meu14-GFP first appeared inside the nuclear region at prophase II, after which it accumulated beside the two SPBs at metaphase II. Thereafter, it formed two ring-shaped structures that surrounded the nucleus at early anaphase II. At post-anaphase II, it disappeared. Meu14-GFP appears to localize at the border of the forespore membrane that later develops into spore walls at the end of sporulation. This was confirmed by coexpressing Spo3-HA, a component of the forespore membrane, with Meu14-GFP. Taken together, we conclude that meu14+ is crucial in meiosis in that it participates in both the nuclear division during meiosis II and the accurate formation of the forespore membrane.
This article has been cited by other articles:
|
|
|
|
 
H. Yan, W. Ge, T. G. Chew, J. Y. Chow, D. McCollum, A. M. Neiman, and M. K. Balasubramanian
The Meiosis-Specific Sid2p-related Protein Slk1p Regulates Forespore Membrane Assembly in Fission Yeast
Mol. Biol. Cell,
September 1, 2008;
19(9):
3676 - 3690.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Nakamura, H. Asakawa, Y. Nakase, J. Kashiwazaki, Y. Hiraoka, and C. Shimoda
Live Observation of Forespore Membrane Formation in Fission Yeast
Mol. Biol. Cell,
August 1, 2008;
19(8):
3544 - 3553.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Nakase, M. Nakamura-Kubo, Y. Ye, A. Hirata, C. Shimoda, and T. Nakamura
Meiotic Spindle Pole Bodies Acquire the Ability to Assemble the Spore Plasma Membrane by Sequential Recruitment of Sporulation-specific Components in Fission Yeast
Mol. Biol. Cell,
June 1, 2008;
19(6):
2476 - 2487.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Perez-Hidalgo, A. E. Rozalen, C. Martin-Castellanos, and S. Moreno
Slk1 is a meiosis-specific Sid2-related kinase that coordinates meiotic nuclear division with growth of the forespore membrane
J. Cell Sci.,
May 1, 2008;
121(9):
1383 - 1392.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Ohtaka, D. Okuzaki, and H. Nojima
Mug27 is a meiosis-specific protein kinase that functions in fission yeast meiosis II and sporulation
J. Cell Sci.,
May 1, 2008;
121(9):
1547 - 1558.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Onishi, M. Iida, T. Koga, S. Yamada, A. Hirata, T. Iwaki, K. Takegawa, Y. Fukui, and H. Tachikawa
Schizosaccharomyces pombe Sst4p, a Conserved Vps27/Hrs Homolog, Functions Downstream of Phosphatidylinositol 3-Kinase Pik3p To Mediate Proper Spore Formation
Eukaryot. Cell,
December 1, 2007;
6(12):
2343 - 2353.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Ohtaka, D. Okuzaki, T. T. Saito, and H. Nojima
Mcp4, a Meiotic Coiled-Coil Protein, Plays a Role in F-Actin Positioning during Schizosaccharomyces pombe Meiosis
Eukaryot. Cell,
June 1, 2007;
6(6):
971 - 983.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Kasama, A. Shigehisa, A. Hirata, T. T. Saito, T. Tougan, D. Okuzaki, and H. Nojima
Spo5/Mug12, a Putative Meiosis-Specific RNA-Binding Protein, Is Essential for Meiotic Progression and Forms Mei2 Dot-Like Nuclear Foci.
Eukaryot. Cell,
August 1, 2006;
5(8):
1301 - 1313.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. T. Saito, D. Okuzaki, and H. Nojima
Mcp5, a meiotic cell cortex protein, is required for nuclear movement mediated by dynein and microtubules in fission yeast
J. Cell Biol.,
April 10, 2006;
173(1):
27 - 33.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. M. Neiman
Ascospore Formation in the Yeast Saccharomyces cerevisiae
Microbiol. Mol. Biol. Rev.,
December 1, 2005;
69(4):
565 - 584.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. T. Saito, T. Tougan, D. Okuzaki, T. Kasama, and H. Nojima
Mcp6, a meiosis-specific coiled-coil protein of Schizosaccharomyces pombe, localizes to the spindle pole body and is required for horsetail movement and recombination
J. Cell Sci.,
January 15, 2005;
118(2):
447 - 459.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. T. Saito, T. Tougan, T. Kasama, D. Okuzaki, and H. Nojima
Mcp7, a meiosis-specific coiled-coil protein of fission yeast, associates with Meu13 and is required for meiotic recombination
Nucleic Acids Res.,
June 21, 2004;
32(11):
3325 - 3339.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Shimoda
Forespore membrane assembly in yeast: coordinating SPBs and membrane trafficking
J. Cell Sci.,
January 22, 2004;
117(3):
389 - 396.
[Abstract]
[Full Text]
[PDF]
|
|
|
© The Company of Biologists Ltd 2003
