The fully linked HTML version of this article has now been published.
JCS ePress
online publication date 23 Apr 2003
doi: 10.1242/jcs.00526
Research Article
Rapid transport of neural intermediate filament protein
Brian T. Helfand,
Patty Loomis,
Miri Yoon,
and
Robert D. Goldman*
* Author for correspondence (e-mail: r-goldman{at}northwestern.edu)
Peripherin is a neural intermediate filament protein that is expressed in peripheral and enteric neurons, as well as in PC12 cells. A determination of the motile properties of peripherin has been undertaken in PC12 cells during different stages of neurite outgrowth. The results reveal that non-filamentous, non-membrane bound peripherin particles and short peripherin intermediate filaments, termed 'squiggles', are transported at high speed throughout PC12 cell bodies, neurites and growth cones. These movements are bi-directional, and the majority require microtubules along with their associated molecular motors, conventional kinesin and cytoplasmic dynein. Our data demonstrate that peripherin particles and squiggles can move as components of a rapid transport system capable of delivering cytoskeletal subunits to the most distal regions of neurites over relatively short time periods.
This article has been cited by other articles:
|
|
|
|
 
S. Sivaramakrishnan, J. V. DeGiulio, L. Lorand, R. D. Goldman, and K. M. Ridge
Micromechanical properties of keratin intermediate filament networks
PNAS,
January 22, 2008;
105(3):
889 - 894.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Kirmse, S. Portet, N. Mucke, U. Aebi, H. Herrmann, and J. Langowski
A Quantitative Kinetic Model for the in Vitro Assembly of Intermediate Filaments from Tetrameric Vimentin
J. Biol. Chem.,
June 22, 2007;
282(25):
18563 - 18572.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. G. Gindhart
Towards an understanding of kinesin-1 dependent transport pathways through the study of protein-protein interactions
Brief Funct Genomic Proteomic,
March 1, 2006;
5(1):
74 - 86.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Chang, Y. Shav-Tal, T. Trcek, R. H. Singer, and R. D. Goldman
Assembling an intermediate filament network by dynamic cotranslation
J. Cell Biol.,
February 27, 2006;
172(5):
747 - 758.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. A. Utton, W. J. Noble, J. E. Hill, B. H. Anderton, and D. P. Hanger
Molecular motors implicated in the axonal transport of tau and {alpha}-synuclein
J. Cell Sci.,
October 15, 2005;
118(20):
4645 - 4654.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Brown, L. Wang, and P. Jung
Stochastic Simulation of Neurofilament Transport in Axons: The "Stop-and-Go" Hypothesis
Mol. Biol. Cell,
September 1, 2005;
16(9):
4243 - 4255.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Yan and A. Brown
Neurofilament Polymer Transport in Axons
J. Neurosci.,
July 27, 2005;
25(30):
7014 - 7021.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Jung, T. M. Chylinski, A. Pimenta, D. Ortiz, and T. B. Shea
Neurofilament Transport Is Dependent on Actin and Myosin
J. Neurosci.,
October 27, 2004;
24(43):
9486 - 9496.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Uchida and A. Brown
Arrival, Reversal, and Departure of Neurofilaments at the Tips of Growing Axons
Mol. Biol. Cell,
September 1, 2004;
15(9):
4215 - 4225.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Windoffer, S. Woll, P. Strnad, and R. E. Leube
Identification of Novel Principles of Keratin Filament Network Turnover in Living Cells
Mol. Biol. Cell,
May 1, 2004;
15(5):
2436 - 2448.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. T. Helfand, L. Chang, and R. D. Goldman
Intermediate filaments are dynamic and motile elements of cellular architecture
J. Cell Sci.,
January 15, 2004;
117(2):
133 - 141.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. T. Helfand, M. G. Mendez, J. Pugh, C. Delsert, and R. D. Goldman
A Role for Intermediate Filaments in Determining and Maintaining the Shape of Nerve Cells
Mol. Biol. Cell,
December 1, 2003;
14(12):
5069 - 5081.
[Abstract]
[Full Text]
[PDF]
|
|
|
© The Company of Biologists Ltd 2003
