spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
JCS ePress online publication date 2 Jul 2003
doi: 10.1242/jcs.00624

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jcs.00624v1
116/16/3399    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Di Fiore, B.
Right arrow Articles by Lavia, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Di Fiore, B.
Right arrow Articles by Lavia, P.

Research Article

Mammalian RanBP1 regulates centrosome cohesion during mitosis


Barbara Di Fiore, Marilena Ciciarello, Rosamaria Mangiacasale, Antonella Palena, Anne-Marie Tassin, Enrico Cundari, and Patrizia Lavia*
* Author for correspondence (e-mail: patrizia.lavia{at}uniroma1.it)

The Ran GTPase plays a central function in control of nucleo-cytoplasmic transport in interphase. Mitotic roles of Ran have also been firmly established in Xenopus oocyte extracts. In this system, Ran-GTP, or the RCC1 exchange factor for Ran, drive spindle assembly by regulating the availability of 'aster-promoting activities'. In previous studies to assess whether the Ran network also influences mitosis in mammalian cells, we found that overexpression of Ran-binding protein 1 (RanBP1), a major effector of Ran, induces multipolar spindles. We now show that these abnormal spindles are generated through loss of cohesion in mitotic centrosomes. Specifically, RanBP1 excess induces splitting of mother and daughter centrioles at spindle poles; the resulting split centrioles can individually organize functional microtubule arrays, giving rise to functional spindle poles. RanBP1-dependent centrosome splitting is specifically induced in mitosis and requires microtubule integrity and Eg5 activity. In addition, we have identified a fraction of RanBP1 at the centrosome. These data indicate that overexpressed RanBP1 interferes with crucial factor(s) that control structural and dynamic features of centrosomes during mitosis and contribute to uncover novel mitotic functions downstream of the Ran network.


This article has been cited by other articles:


Home page
 Mol. Cell. Biol.Home page
A. Neuber, J. Franke, A. Wittstruck, G. Schlenstedt, T. Sommer, and K. Stade
Nuclear Export Receptor Xpo1/Crm1 Is Physically and Functionally Linked to the Spindle Pole Body in Budding Yeast
Mol. Cell. Biol., September 1, 2008; 28(17): 5348 - 5358.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
S. Yang, X. Liu, Y. Yin, M. N. Fukuda, and J. Zhou
Tastin is required for bipolar spindle assembly and centrosome integrity during mitosis
FASEB J, June 1, 2008; 22(6): 1960 - 1972.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
A. Tedeschi, M. Ciciarello, R. Mangiacasale, E. Roscioli, W. M. Rensen, and P. Lavia
RANBP1 localizes a subset of mitotic regulatory factors on spindle microtubules and regulates chromosome segregation in human cells
J. Cell Sci., November 1, 2007; 120(21): 3748 - 3761.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
S. Fan, V. Fogg, Q. Wang, X.-W. Chen, C.-J. Liu, and B. Margolis
A novel Crumbs3 isoform regulates cell division and ciliogenesis via importin {beta} interactions
J. Cell Biol., July 24, 2007; 178(3): 387 - 398.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. J. Albee, W. Tao, and C. Wiese
Phosphorylation of Maskin by Aurora-A Is Regulated by RanGTP and Importin beta
J. Biol. Chem., December 15, 2006; 281(50): 38293 - 38301.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
J. Joseph
Ran at a glance.
J. Cell Sci., September 1, 2006; 119(Pt 17): 3481 - 3484.
[Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
A. V. Orjalo, A. Arnaoutov, Z. Shen, Y. Boyarchuk, S. G. Zeitlin, B. Fontoura, S. Briggs, M. Dasso, and D. J. Forbes
The Nup107-160 Nucleoporin Complex Is Required for Correct Bipolar Spindle Assembly
Mol. Biol. Cell, September 1, 2006; 17(9): 3806 - 3818.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
A. Christodoulou, C. W. Lederer, T. Surrey, I. Vernos, and N. Santama
Motor protein KIFC5A interacts with Nubp1 and Nubp2, and is implicated in the regulation of centrosome duplication
J. Cell Sci., May 15, 2006; 119(10): 2035 - 2047.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
R. V. Silverman-Gavrila and A. Wilde
Ran Is Required before Metaphase for Spindle Assembly and Chromosome Alignment and after Metaphase for Chromosome Segregation and Spindle Midbody Organization
Mol. Biol. Cell, April 1, 2006; 17(4): 2069 - 2080.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
J. Yang, M. Adamian, and T. Li
Rootletin Interacts with C-Nap1 and May Function as a Physical Linker between the Pair of Centrioles/Basal Bodies in Cells
Mol. Biol. Cell, February 1, 2006; 17(2): 1033 - 1040.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J.-M. Peloponese Jr., K. Haller, A. Miyazato, and K.-T. Jeang
Abnormal centrosome amplification in cells through the targeting of Ran-binding protein-1 by the human T cell leukemia virus type-1 Tax oncoprotein
PNAS, December 27, 2005; 102(52): 18974 - 18979.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
Y.-K. Cao, Z.-S. Zhong, D.-Y. Chen, G.-X. Zhang, H. Schatten, and Q.-Y. Sun
Cell cycle-dependent localization and possible roles of the small GTPase Ran in mouse oocyte maturation, fertilization and early cleavage
Reproduction, October 1, 2005; 130(4): 431 - 440.
[Abstract] [Full Text] [PDF]

Home page
 GENES CELLSHome page
T. Nishimura, M. Takahashi, H.-S. Kim, H. Mukai, and Y. Ono
Centrosome-targeting region of CG-NAP causes centrosome amplification by recruiting cyclin E-cdk2 complex
Genes Cells, January 1, 2005; 10(1): 75 - 86.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
M. Ciciarello, R. Mangiacasale, C. Thibier, G. Guarguaglini, E. Marchetti, B. Di Fiore, and P. Lavia
Importin {beta} is transported to spindle poles during mitosis and regulates Ran-dependent spindle assembly factors in mammalian cells
J. Cell Sci., December 15, 2004; 117(26): 6511 - 6522.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
F. BALUSKA, D. VOLKMANN, and P. W. BARLOW
Eukaryotic Cells and their Cell Bodies: Cell Theory Revised
Ann. Bot., July 1, 2004; 94(1): 9 - 32.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
L. Cassimeris and J. Morabito
TOGp, the Human Homolog of XMAP215/Dis1, Is Required for Centrosome Integrity, Spindle Pole Organization, and Bipolar Spindle Assembly
Mol. Biol. Cell, April 1, 2004; 15(4): 1580 - 1590.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
G. Keryer, B. Di Fiore, C. Celati, K. F. Lechtreck, M. Mogensen, A. Delouvee, P. Lavia, M. Bornens, and A.-M. Tassin
Part of Ran Is Associated with AKAP450 at the Centrosome: Involvement in Microtubule-organizing Activity
Mol. Biol. Cell, October 1, 2003; 14(10): 4260 - 4271.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2003