spacer gif spacer gif spacer gif spacer gif Propose a workshop for 2011 spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
JCS ePress online publication date 22 Jul 2003
doi: 10.1242/jcs.00680

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jcs.00680v1
116/17/3571    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nelson, C. M.
Right arrow Articles by Chen, C. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nelson, C. M.
Right arrow Articles by Chen, C. S.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research Article

VE-cadherin simultaneously stimulates and inhibits cell proliferation by altering cytoskeletal structure and tension


Celeste M. Nelson and Christopher S. Chen*
* Author for correspondence (e-mail: cchen{at}bme.jhu.edu)

Engagement of vascular endothelial (VE)-cadherin leads to the cessation of proliferation commonly known as 'contact inhibition'. We show that VE-cadherin inhibits growth by mediating changes in cell adhesion to the extracellular matrix. Increasing cell-cell contact decreased cell spreading and proliferation, which was reversed by blocking engagement of VE-cadherin. Using a new system to prevent the cadherin-induced changes in cell spreading, we revealed that VE-cadherin paradoxically increased proliferation. Treating cells with inhibitors of PKC and MEK abrogated the stimulatory signal at concentrations that disrupted the formation of actin fibers across the cell-cell contact. Directly disrupting actin fibers, blocking actin-myosin-generated tension, or inhibiting signaling through Rho specifically inhibited the cadherin-induced proliferative signal. By progressively altering the degree to which cell-cell contact inhibited cell spreading, we show that cell-cell contact ultimately increased or decreased the overall proliferation rate of the population by differentially shifting the balance between the two opposing proliferative cues. The existence of opposing growth signals induced by VE-cadherin that are both mediated through crosstalk with cytoskeletal structure highlights the complex interplay of mechanical and chemical signals with which cells navigate in their physical microenvironment.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
R. A. Desai, L. Gao, S. Raghavan, W. F. Liu, and C. S. Chen
Cell polarity triggered by cell-cell adhesion via E-cadherin
J. Cell Sci., April 1, 2009; 122(7): 905 - 911.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
L.-W. Qian, W. Greene, F. Ye, and S.-J. Gao
Kaposi's Sarcoma-Associated Herpesvirus Disrupts Adherens Junctions and Increases Endothelial Permeability by Inducing Degradation of VE-Cadherin
J. Virol., December 1, 2008; 82(23): 11902 - 11912.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. R. Pennington, B. J. Foster, S. R. Hawley, R. E. Jenkins, O. Zolle, M. R. H. White, C. J. McNamee, P. Sheterline, and A. W. M. Simpson
Cell Shape-dependent Control of Ca2+ Influx and Cell Cycle Progression in Swiss 3T3 Fibroblasts
J. Biol. Chem., November 2, 2007; 282(44): 32112 - 32120.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
W. F. Liu, C. M. Nelson, D. M. Pirone, and C. S. Chen
E-cadherin engagement stimulates proliferation via Rac1
J. Cell Biol., May 8, 2006; 173(3): 431 - 441.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
C. M. Nelson, D. M. Pirone, J. L. Tan, and C. S. Chen
Vascular Endothelial-Cadherin Regulates Cytoskeletal Tension, Cell Spreading, and Focal Adhesions by Stimulating RhoA
Mol. Biol. Cell, June 1, 2004; 15(6): 2943 - 2953.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2003