The fully linked HTML version of this article has now been published.
JCS ePress
online publication date 12 Aug 2003
doi: 10.1242/jcs.00705
Research Article
Talin loss-of-function uncovers roles in cell contractility and migration in C. elegans
Erin J. Cram,
Scott G. Clark,
and
Jean E. Schwarzbauer*
* Author for correspondence (e-mail: jschwarzbauer{at}molbio.princeton.edu)
Integrin receptors for extracellular matrix transmit mechanical and biochemical information through molecular connections to the actin cytoskeleton and to several intracellular signaling pathways. In Caenorhabditis elegans, integrins are essential for embryonic development, muscle cell adhesion and contraction, and migration of nerve cell axons and gonadal distal tip cells. To identify key components involved in distal tip cell migration, we are using an RNA interference (RNAi)-based genetic screen for deformities in gonad morphogenesis. We have found that talin, a cytoskeletal-associated protein and focal adhesion component, is expressed in the distal tip cell and plays a central role in regulating its migration. Reduction of talin expression caused severe defects in gonad formation because of aberrant distal tip cell migration and also disrupted oocyte maturation and gonad sheath cell structure. Contractile muscle cells showed disorganization of the actin cytoskeleton leading to complete paralysis, a phenotype that was also observed with depletion of pat-2 and pat-3 integrins. These in vivo analyses show that talin is required not only for strong adhesion and cytoskeletal organization by contractile cells, but also for dynamic regulation of integrin signals during cell migration. In addition, induction of distal tip cell migration defects by bacterial RNAi in C. elegans provides an effective screen to identify genes involved in integrin signaling and function.
This article has been cited by other articles:
|
|
|
|
 
K. Ono, S. Yamashiro, and S. Ono
Essential role of ADF/cofilin for assembly of contractile actin networks in the C. elegans somatic gonad
J. Cell Sci.,
August 15, 2008;
121(16):
2662 - 2670.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. L. Helsten, T. A. Bunch, H. Kato, J. Yamanouchi, S. H. Choi, A. L. Jannuzi, C. C. Feral, M. H. Ginsberg, D. L. Brower, and S. J. Shattil
Differences in Regulation of Drosophila and Vertebrate Integrin Affinity by Talin
Mol. Biol. Cell,
August 1, 2008;
19(8):
3589 - 3598.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. J. Conti, A. Felder, S. Monkley, M. Schwander, M. R. Wood, R. Lieber, D. Critchley, and U. Muller
Progressive myopathy and defects in the maintenance of myotendinous junctions in mice that lack talin 1 in skeletal muscle
Development,
June 1, 2008;
135(11):
2043 - 2053.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. G. Petrich, P. Marchese, Z. M. Ruggeri, S. Spiess, R. A.M. Weichert, F. Ye, R. Tiedt, R. C. Skoda, S. J. Monkley, D. R. Critchley, et al.
Talin is required for integrin-mediated platelet function in hemostasis and thrombosis
J. Exp. Med.,
December 24, 2007;
204(13):
3103 - 3111.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Sottile, F. Shi, I. Rublyevska, H.-Y. Chiang, J. Lust, and J. Chandler
Fibronectin-dependent collagen I deposition modulates the cell response to fibronectin
Am J Physiol Cell Physiol,
December 1, 2007;
293(6):
C1934 - C1946.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Sun, K. Ling, M. P. Wagoner, and R. A. Anderson
Type I{gamma} phosphatidylinositol phosphate kinase is required for EGF-stimulated directional cell migration
J. Cell Biol.,
July 10, 2007;
178(2):
297 - 308.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. M. Meighan and J. E. Schwarzbauer
Control of C. elegans hermaphrodite gonad size and shape by vab-3/Pax6-mediated regulation of integrin receptors
Genes & Dev.,
July 1, 2007;
21(13):
1615 - 1620.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. J. Cram, H. Shang, and J. E. Schwarzbauer
A systematic RNA interference screen reveals a cell migration gene network in C. elegans
J. Cell Sci.,
December 1, 2006;
119(23):
4811 - 4818.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Ju, Q. Zheng, and R. D. Cummings
Identification of core 1 O-glycan T-synthase from Caenorhabditis elegans
Glycobiology,
October 1, 2006;
16(10):
947 - 958.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Hashmi, J. Zhang, Y. Oksov, Q. Ji, and S. Lustigman
The Caenorhabditis elegans CPI-2a Cystatin-like Inhibitor Has an Essential Regulatory Role during Oogenesis and Fertilization
J. Biol. Chem.,
September 22, 2006;
281(38):
28415 - 28429.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Pylayeva and F. G. Giancotti
Development requires activation but not phosphorylation of beta1 integrins.
Genes & Dev.,
May 1, 2006;
20(9):
1057 - 1060.
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Chen, Z. Zou, K. L. Sarratt, D. Zhou, M. Zhang, E. Sebzda, D. A. Hammer, and M. L. Kahn
In vivo beta1 integrin function requires phosphorylation-independent regulation by cytoplasmic tyrosines
Genes & Dev.,
April 15, 2006;
20(8):
927 - 932.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Patel, A. R. Gingras, A. A. Bobkov, L. M. Fujimoto, M. Zhang, R. C. Liddington, D. Mazzeo, J. Emsley, G. C. K. Roberts, I. L. Barsukov, et al.
The Activity of the Vinculin Binding Sites in Talin Is Influenced by the Stability of the Helical Bundles That Make Up The Talin Rod
J. Biol. Chem.,
March 17, 2006;
281(11):
7458 - 7467.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. R. Gingras, W. H. Ziegler, R. Frank, I. L. Barsukov, G. C. K. Roberts, D. R. Critchley, and J. Emsley
Mapping and Consensus Sequence Identification for Multiple Vinculin Binding Sites within the Talin Rod
J. Biol. Chem.,
November 4, 2005;
280(44):
37217 - 37224.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. G. Arias-Salgado, S. Lizano, S. J. Shattil, and M. H. Ginsberg
Specification of the Direction of Adhesive Signaling by the Integrin {beta} Cytoplasmic Domain
J. Biol. Chem.,
August 19, 2005;
280(33):
29699 - 29707.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. L. Hooper and J. B. Thuma
Invertebrate Muscles: Muscle Specific Genes and Proteins
Physiol Rev,
July 1, 2005;
85(3):
1001 - 1060.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. A. Calderwood, V. Tai, G. Di Paolo, P. De Camilli, and M. H. Ginsberg
Competition for Talin Results in Trans-dominant Inhibition of Integrin Activation
J. Biol. Chem.,
July 9, 2004;
279(28):
28889 - 28895.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Ono and S. Ono
Tropomyosin and Troponin Are Required for Ovarian Contraction in the Caenorhabditis elegans Reproductive System
Mol. Biol. Cell,
June 1, 2004;
15(6):
2782 - 2793.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. A. Cox and J. Hardin
Sticky worms: adhesion complexes in C. elegans
J. Cell Sci.,
April 15, 2004;
117(10):
1885 - 1897.
[Abstract]
[Full Text]
[PDF]
|
|
|
© The Company of Biologists Ltd 2003
