spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
JCS ePress online publication date 2 Sep 2003
doi: 10.1242/jcs.00724

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jcs.00724v1
116/20/4201    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Roczniak-Ferguson, A.
Right arrow Articles by Reynolds, A. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Roczniak-Ferguson, A.
Right arrow Articles by Reynolds, A. B.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research Article

Regulation of p120-catenin nucleocytoplasmic shuttling activity


Agnes Roczniak-Ferguson and Albert B. Reynolds*
* Author for correspondence (e-mail: al.reynolds{at}vanderbilt.edu)

P120-catenin is the prototypic member of a subfamily of Armadillo repeat domain (Arm domain) proteins involved in cell-cell adhesion. Interestingly, all members of the p120 subfamily have also been observed in the nucleus, suggesting that they have additional roles that have yet to be determined. Here, we have developed a novel model system for studying the nucleocytoplasmic shuttling capabilities of p120. We show that simultaneous deletion of both of the conventional nuclear localization sequences (NLSs) in p120 had little effect on its nuclear localization. Instead, the Armadillo repeat domain was essential, and deletion of Arm repeat 3 or Arm repeat 5 eliminated nuclear entry despite the presence of both NLSs. In addition, deletion of Arm repeat 8 resulted in constitutive nuclear localization of p120-3A in both E-cadherin-positive and -negative cell lines. Thus, the core shuttling functions are dependent on the Arm domain. We have also identified two regions within the N-terminus of p120 that modulate nuclear shuttling dynamics of p120. In cadherin-deficient cells, normal epithelial morphology could be restored by both WT-E-cadherin and p120 uncoupled E-cadherin mutants, but only WT-E-cadherin strongly reduced nuclear localization of p120. Moreover, structural changes in p120 that reduced its affinity for E-cadherin increased p120 nuclear localization. Thus, reduced shuttling in the presence of E-cadherin is principally due to sequestration, a condition that is probably dynamic under normal circumstances but completely lost in metastatic cells that have downregulated E-cadherin. Notably, Arm repeats 3 and 5 are necessary for both E-cadherin binding and nuclear translocation, indicating that these repeats have dual roles. Surprisingly, in the absence of E-cadherin there was significant colocalization of cytoplasmic p120 with elements of the tubulin cytoskeleton, particularly in perinuclear locations. Depolymerizing microtubules with nocodazole increased nuclear p120, whereas stabilizing tubulin with taxol reduced nuclear p120 and strongly increased p120 association with microtubules. Thus, p120 has intrinsic nucleocytoplasmic shuttling activity that is modulated, in part, by extrinsic factors such as cadherin binding and interactions with the microtubule network.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 Mol. Endocrinol.Home page
S. J. Lee, C. Chae, and M. M. Wang
p150/Glued Modifies Nuclear Estrogen Receptor Function
Mol. Endocrinol., May 1, 2009; 23(5): 620 - 629.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Abu-Elneel, T. Ochiishi, M. Medina, M. Remedi, L. Gastaldi, A. Caceres, and K. S. Kosik
A {delta}-Catenin Signaling Pathway Leading to Dendritic Protrusions
J. Biol. Chem., November 21, 2008; 283(47): 32781 - 32791.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Pathol.Home page
J Paredes, A L Correia, A S Ribeiro, F Milanezi, J Cameselle-Teijeiro, and F C Schmitt
Breast carcinomas that co-express E- and P-cadherin are associated with p120-catenin cytoplasmic localisation and poor patient survival
J. Clin. Pathol., July 1, 2008; 61(7): 856 - 862.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. C. Ferber, M. Kajita, A. Wadlow, L. Tobiansky, C. Niessen, H. Ariga, J. Daniel, and Y. Fujita
A Role for the Cleaved Cytoplasmic Domain of E-cadherin in the Nucleus
J. Biol. Chem., May 9, 2008; 283(19): 12691 - 12700.
[Abstract] [Full Text] [PDF]

Home page
 GENES CELLSHome page
T. Ichii and M. Takeichi
p120-catenin regulates microtubule dynamics and cell migration in a cadherin-independent manner
Genes Cells, July 1, 2007; 12(7): 827 - 839.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
C. R. Hosking, F. Ulloa, C. Hogan, E. C. Ferber, A. Figueroa, K. Gevaert, W. Birchmeier, J. Briscoe, and Y. Fujita
The Transcriptional Repressor Glis2 Is a Novel Binding Partner for p120 Catenin
Mol. Biol. Cell, May 1, 2007; 18(5): 1918 - 1927.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
B. Manavathi, F. Acconcia, S. K. Rayala, and R. Kumar
An inherent role of microtubule network in the action of nuclear receptor
PNAS, October 24, 2006; 103(43): 15981 - 15986.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
K. Strumane, A. Bonnomet, C. Stove, R. Vandenbroucke, B. Nawrocki-Raby, E. Bruyneel, M. Mareel, P. Birembaut, G. Berx, and F. van Roy
E-Cadherin Regulates Human Nanos1, which Interacts with p120ctn and Induces Tumor Cell Migration and Invasion.
Cancer Res., October 15, 2006; 66(20): 10007 - 10015.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
H.-N. Fournier, S. Dupe-Manet, D. Bouvard, F. Luton, S. Degani, M. R. Block, S. F. Retta, and C. Albiges-Rizo
Nuclear Translocation of Integrin Cytoplasmic Domain-associated Protein 1 Stimulates Cellular Proliferation
Mol. Biol. Cell, April 1, 2005; 16(4): 1859 - 1871.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
K. F. Kelly, A. A. Otchere, M. Graham, and J. M. Daniel
Nuclear import of the BTB/POZ transcriptional regulator Kaiso
J. Cell Sci., December 1, 2004; 117(25): 6143 - 6152.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
K. F. Kelly, C. M. Spring, A. A. Otchere, and J. M. Daniel
NLS-dependent nuclear localization of p120ctn is necessary to relieve Kaiso-mediated transcriptional repression
J. Cell Sci., June 1, 2004; 117(13): 2675 - 2686.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Yanagisawa, I. N. Kaverina, A. Wang, Y. Fujita, A. B. Reynolds, and P. Z. Anastasiadis
A Novel Interaction between Kinesin and p120 Modulates p120 Localization and Function
J. Biol. Chem., March 5, 2004; 279(10): 9512 - 9521.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. M. Franz and A. J. Ridley
p120 Catenin Associates with Microtubules: INVERSE RELATIONSHIP BETWEEN MICROTUBULE BINDING AND RHO GTPase REGULATION
J. Biol. Chem., February 20, 2004; 279(8): 6588 - 6594.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2003