spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
JCS ePress online publication date 9 Sep 2003
doi: 10.1242/jcs.00726

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jcs.00726v1
116/21/4331    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Décavé, E.
Right arrow Articles by Bruckert, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Décavé, E.
Right arrow Articles by Bruckert, F.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research Article

Shear flow-induced motility of Dictyostelium discoideum cells on solid substrate


Emmanuel Décavé, Didier Rieu, Jérémie Dalous, Sébastien Fache, Yves Bréchet, Bertrand Fourcade, Michel Satre, and Franz Bruckert*
* Author for correspondence (e-mail: fbruckert{at}cea.fr)

Application of a mild hydrodynamic shear stress to Dicytostelium discoideum cells, unable to detach cells passively from the substrate, triggers a cellular response consisting of steady membrane peeling at the rear edge of the cell and periodic cell contact extensions at its front edge. Both processes require an active actin cytoskeleton. The cell movement induced by the hydrodynamic forces is very similar to amoeboid cell motion during chemotaxis, as for its kinematic parameters and for the involvement of phosphatidylinositol(3,4,5)-trisphosphate internal gradient to maintain cell polarity. Inhibition of phosphoinositide 3-kinases by LY294002 randomizes the orientation of cell movement with respect to the flow without modifying cell speed. Two independent signaling pathways are, therefore, induced in D. discoideum in response to external forces. The first increases the frequency of pseudopodium extension, whereas the second redirects the actin cytoskeleton polymerization machinery to the edge opposite to the stressed side of the cell.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 J. Immunol.Home page
M. Phillipson, B. Heit, S. A. Parsons, B. Petri, S. C. Mullaly, P. Colarusso, R. M. Gower, G. Neely, S. I. Simon, and P. Kubes
Vav1 Is Essential for Mechanotactic Crawling and Migration of Neutrophils out of the Inflamed Microvasculature
J. Immunol., June 1, 2009; 182(11): 6870 - 6878.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
L. E. Goldfinger, E. Tzima, R. Stockton, W. B. Kiosses, K. Kinbara, E. Tkachenko, E. Gutierrez, A. Groisman, P. Nguyen, S. Chien, et al.
Localized {alpha}4 Integrin Phosphorylation Directs Shear Stress-Induced Endothelial Cell Alignment
Circ. Res., July 18, 2008; 103(2): 177 - 185.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
S. Fache, J. Dalous, M. Engelund, C. Hansen, F. Chamaraux, B. Fourcade, M. Satre, P. Devreotes, and F. Bruckert
Calcium mobilization stimulates Dictyostelium discoideum shear-flow-induced cell motility
J. Cell Sci., August 1, 2005; 118(15): 3445 - 3458.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
J. Pu and M. Zhao
Golgi polarization in a strong electric field
J. Cell Sci., March 15, 2005; 118(6): 1117 - 1128.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
L. Gebbie, M. Benghezal, S. Cornillon, R. Froquet, N. Cherix, M. Malbouyres, Y. Lefkir, C. Grangeasse, S. Fache, J. Dalous, et al.
Phg2, a Kinase Involved in Adhesion and Focal Site Modeling in Dictyostelium
Mol. Biol. Cell, August 1, 2004; 15(8): 3915 - 3925.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2003