spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
JCS ePress online publication date 20 Jan 2004
doi: 10.1242/jcs.00878

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jcs.00878v1
117/5/723    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Liang, W.
Right arrow Articles by Fishman, P. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Liang, W.
Right arrow Articles by Fishman, P. H.

Research Article

Differences in endosomal targeting of human {beta}1- and {beta}2-adrenergic receptors following clathrin-mediated endocytosis


Wei Liang, Patricia K. Curran, Quang Hoang, R. Travis Moreland, and Peter H. Fishman*
* Author for correspondence (e-mail: fishmanp{at}ninds.nih.gov)

The {beta}2-adrenergic receptor ({beta}2AR) undergoes agonist-mediated endocytosis via clathrin-coated pits by a process dependent on both arrestins and dynamin. Internalization of some G protein-coupled receptors, however, is independent of arrestins and/or dynamin and through other membrane microdomains such as caveolae or lipid rafts. The human {beta}1AR is less susceptible to agonist-mediated internalization than the {beta}2-subtype, and its endocytic route, which is unknown, may be different. We have found that (i) co-expression of arrestin-2 or -3 enhanced the internalization of both subtypes whereas co-expression of dominant-negative mutants of arrestin-2 or dynamin impaired their internalization, as did inhibitors of clathrin-mediated endocytosis. (ii) Agonist stimulation increased the phosphorylation of {beta}2AR but not {beta}1AR. (iii) In response to agonist, each subtype redistributed from the cell surface to a distinct population of cytoplasmic vesicles; those containing {beta}1AR were smaller and closer to the plasma membrane whereas those containing {beta}2AR were larger and more perinuclear. (iv) When subcellular fractions from agonist-treated cells were separated by sucrose density gradient centrifugation, all of the internalized {beta}2AR appeared in the lighter endosomal-containing fractions whereas some of the internalized {beta}1AR remained in the denser plasma membrane-containing fractions. (v) Both subtypes recycled with similar kinetics back to the cell surface upon removal of agonist; however, recycling of {beta}2AR but not {beta}1AR was inhibited by monensin. Based on these results, we propose that the internalization of {beta}1AR is both arrestin- and dynamin-dependent and follows the same clathrin-mediated endocytic pathway as {beta}2AR. But during or after endocytosis, {beta}1AR and {beta}2AR are sorted into different endosomal compartments.


This article has been cited by other articles:


Home page
 J. Immunol.Home page
A. Meiser, A. Mueller, E. L. Wise, E. M. McDonagh, S. J. Petit, N. Saran, P. C. Clark, T. J. Williams, and J. E. Pease
The Chemokine Receptor CXCR3 Is Degraded following Internalization and Is Replenished at the Cell Surface by De Novo Synthesis of Receptor
J. Immunol., May 15, 2008; 180(10): 6713 - 6724.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
F. Philip, P. Sengupta, and S. Scarlata
Signaling through a G Protein-coupled Receptor and Its Corresponding G Protein Follows a Stoichiometrically Limited Model
J. Biol. Chem., June 29, 2007; 282(26): 19203 - 19216.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
T. M. Tran, J. Friedman, F. Baameur, B. J. Knoll, R. H. Moore, and R. B. Clark
Characterization of beta2-Adrenergic Receptor Dephosphorylation: Comparison with the Rate of Resensitization
Mol. Pharmacol., January 1, 2007; 71(1): 47 - 60.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. Muro, M. Mateescu, C. Gajewski, M. Robinson, V. R. Muzykantov, and M. Koval
Control of intracellular trafficking of ICAM-1-targeted nanocarriers by endothelial Na+/H+ exchanger proteins
Am J Physiol Lung Cell Mol Physiol, May 1, 2006; 290(5): L809 - L817.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
M. Zaitseva, T. Romantseva, J. Manischewitz, J. Wang, D. Goucher, and H. Golding
Increased CXCR4-dependent HIV-1 fusion in activated T cells: role of CD4/CXCR4 association
J. Leukoc. Biol., December 1, 2005; 78(6): 1306 - 1317.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
L. Wang, C. G. Radu, L. V. Yang, L. A. Bentolila, M. Riedinger, and O. N. Witte
Lysophosphatidylcholine-induced Surface Redistribution Regulates Signaling of the Murine G Protein-coupled Receptor G2A
Mol. Biol. Cell, May 1, 2005; 16(5): 2234 - 2247.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
W. Liang and P. H. Fishman
Resistance of the Human {beta}1-Adrenergic Receptor to Agonist-induced Ubiquitination: A MECHANISM FOR IMPAIRED RECEPTOR DEGRADATION
J. Biol. Chem., November 5, 2004; 279(45): 46882 - 46889.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2004