The fully linked HTML version of this article has now been published.
JCS ePress
online publication date 6 Jan 2004
doi: 10.1242/jcs.00893
Research Article
Mechanism of recruiting Sec6/8 (exocyst) complex to the apical junctional complex during polarization of epithelial cells
Charles Yeaman*,
Kent K. Grindstaff,
and
W. James Nelson
* Author for correspondence (e-mail: charles-yeaman{at}uiowa.edu)
Sec6/8 (exocyst) complex regulates vesicle delivery and polarized membrane growth in a variety of cells, but mechanisms regulating Sec6/8 localization are unknown. In epithelial cells, Sec6/8 complex is recruited to cell-cell contacts with a mixture of junctional proteins, but then sorts out to the apex of the lateral membrane with components of tight junction and nectin complexes. Sec6/8 complex fractionates in a high molecular mass complex with tight junction proteins and a portion of E-cadherin, and co-immunoprecipitates with cell surface-labeled E-cadherin and nectin-2
. Recruitment of Sec6/8 complex to cell-cell contacts can be achieved in fibroblasts when E-cadherin and nectin-2 are co-expressed. These results support a model in which localized recruitment of Sec6/8 complex to the plasma membrane by specific cell-cell adhesion complexes defines a site for vesicle delivery and polarized membrane growth during development of epithelial cell polarity.
This article has been cited by other articles:
|
|
|
|
 
M. S. Balda and K. Matter
Tight junctions at a glance
J. Cell Sci.,
November 15, 2008;
121(22):
3677 - 3682.
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. Cao, C. Li, J. N. Higginbotham, J. L. Franklin, D. L. Tabb, R. Graves-Deal, S. Hill, K. Cheek, W. G. Jerome, L. A. Lapierre, et al.
Use of Fluorescence-activated Vesicle Sorting for Isolation of Naked2-associated, Basolaterally Targeted Exocytic Vesicles for Proteomics Analysis
Mol. Cell. Proteomics,
September 1, 2008;
7(9):
1651 - 1667.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. S. Spiczka and C. Yeaman
Ral-regulated interaction between Sec5 and paxillin targets Exocyst to focal complexes during cell migration
J. Cell Sci.,
September 1, 2008;
121(17):
2880 - 2891.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Hala, R. Cole, L. Synek, E. Drdova, T. Pecenkova, A. Nordheim, T. Lamkemeyer, J. Madlung, F. Hochholdinger, J. E. Fowler, et al.
An Exocyst Complex Functions in Plant Cell Growth in Arabidopsis and Tobacco
PLANT CELL,
May 1, 2008;
20(5):
1330 - 1345.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. M. Torkko, A. Manninen, S. Schuck, and K. Simons
Depletion of apical transport proteins perturbs epithelial cyst formation and ciliogenesis
J. Cell Sci.,
April 15, 2008;
121(8):
1193 - 1203.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. M. Saaf, J. M. Halbleib, X. Chen, S. T. Yuen, S. Y. Leung, W. J. Nelson, and P. O. Brown
Parallels between Global Transcriptional Programs of Polarizing Caco-2 Intestinal Epithelial Cells In Vitro and Gene Expression Programs in Normal Colon and Colon Cancer
Mol. Biol. Cell,
November 1, 2007;
18(11):
4245 - 4260.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. P. Manderson, J. G. Kay, L. A. Hammond, D. L. Brown, and J. L. Stow
Subcompartments of the macrophage recycling endosome direct the differential secretion of IL-6 and TNF{alpha}
J. Cell Biol.,
October 3, 2007;
178(1):
57 - 69.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Oztan, M. Silvis, O. A. Weisz, N. A. Bradbury, S.-C. Hsu, J. R. Goldenring, C. Yeaman, and G. Apodaca
Exocyst Requirement for Endocytic Traffic Directed Toward the Apical and Basolateral Poles of Polarized MDCK Cells
Mol. Biol. Cell,
October 1, 2007;
18(10):
3978 - 3992.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. M. Klerks, M. van Gent-Pelzer, E. Franz, C. Zijlstra, and A. H. C. van Bruggen
Physiological and Molecular Responses of Lactuca sativa to Colonization by Salmonella enterica Serovar Dublin
Appl. Envir. Microbiol.,
August 1, 2007;
73(15):
4905 - 4914.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Pommereit and F. S. Wouters
An NGF-induced Exo70-TC10 complex locally antagonises Cdc42-mediated activation of N-WASP to modulate neurite outgrowth
J. Cell Sci.,
August 1, 2007;
120(15):
2694 - 2705.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Li, M. Hao, Z. Cao, W. Ding, R. Graves-Deal, J. Hu, D. W. Piston, and R. J. Coffey
Naked2 Acts as a Cargo Recognition and Targeting Protein to Ensure Proper Delivery and Fusion of TGF-{alpha} containing Exocytic Vesicles at the Lower Lateral Membrane of Polarized MDCK Cells
Mol. Biol. Cell,
August 1, 2007;
18(8):
3081 - 3093.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. N. Nejsum and W. J. Nelson
A molecular mechanism directly linking E-cadherin adhesion to initiation of epithelial cell surface polarity
J. Cell Biol.,
July 10, 2007;
178(2):
323 - 335.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Shmuel, E. Nodel-Berner, T. Hyman, A. Rouvinski, and Y. Altschuler
Caveolin 2 Regulates Endocytosis and Trafficking of the M1 Muscarinic Receptor in MDCK Epithelial Cells
Mol. Biol. Cell,
May 1, 2007;
18(5):
1570 - 1585.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Q. Wang, X.-W. Chen, and B. Margolis
PALS1 Regulates E-Cadherin Trafficking in Mammalian Epithelial Cells
Mol. Biol. Cell,
March 1, 2007;
18(3):
874 - 885.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Anitei, M. Ifrim, M.-A. Ewart, A. E. Cowan, J. H. Carson, R. Bansal, and S. E. Pfeiffer
A role for Sec8 in oligodendrocyte morphological differentiation
J. Cell Sci.,
March 1, 2006;
119(5):
807 - 818.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Sztul and V. Lupashin
Role of tethering factors in secretory membrane traffic
Am J Physiol Cell Physiol,
January 1, 2006;
290(1):
C11 - C26.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. A. Cole, L. Synek, V. Zarsky, and J. E. Fowler
SEC8, a Subunit of the Putative Arabidopsis Exocyst Complex, Facilitates Pollen Germination and Competitive Pollen Tube Growth
Plant Physiology,
August 1, 2005;
138(4):
2005 - 2018.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Beronja, P. Laprise, O. Papoulas, M. Pellikka, J. Sisson, and U. Tepass
Essential function of Drosophila Sec6 in apical exocytosis of epithelial photoreceptor cells
J. Cell Biol.,
May 23, 2005;
169(4):
635 - 646.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Murthy, R. Ranjan, N. Denef, M. E. L. Higashi, T. Schupbach, and T. L. Schwarz
Sec6 mutations and the Drosophila exocyst complex
J. Cell Sci.,
March 15, 2005;
118(6):
1139 - 1150.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Vogelmann and W. J. Nelson
Fractionation of the Epithelial Apical Junctional Complex: Reassessment of Protein Distributions in Different Substructures
Mol. Biol. Cell,
February 1, 2005;
16(2):
701 - 716.
[Abstract]
[Full Text]
[PDF]
|
|
|
© The Company of Biologists Ltd 2004
