The fully linked HTML version of this article has now been published.
JCS ePress
online publication date 3 Feb 2004
doi: 10.1242/jcs.00912
Research Article
The surface of articular cartilage contains a progenitor cell population
Gary P. Dowthwaite,
Joanna C. Bishop,
Samantha N. Redman,
Ilyas M. Khan,
Paul Rooney,
Darrell J.R. Evans,
Laura Haughton,
Zubeyde Bayram,
Sam Boyer,
Brian Thomson,
Michael S. Wolfe,
and
Charles W. Archer*
* Author for correspondence (e-mail: archer{at}cardiff.ac.uk)
It is becoming increasingly apparent that articular cartilage growth is achieved by apposition from the articular surface. For such a mechanism to occur, a population of stem/progenitor cells must reside within the articular cartilage to provide transit amplifying progeny for growth. Here, we report on the isolation of an articular cartilage progenitor cell from the surface zone of articular cartilage using differential adhesion to fibronectin. This population of cells exhibits high affinity for fibronectin, possesses a high colony-forming efficiency and expresses the cell fate selector gene Notch 1. Inhibition of Notch signalling abolishes colony forming ability whilst activated Notch rescues this inhibition. The progenitor population also exhibits phenotypic plasticity in its differentiation pathway in an embryonic chick tracking system, such that chondroprogenitors can engraft into a variety of connective tissue types including bone, tendon and perimysium. The identification of a chondrocyte subpopulation with progenitor-like characteristics will allow for advances in our understanding of both cartilage growth and maintenance as well as provide novel solutions to articular cartilage repair.
This article has been cited by other articles:
|
|
|
|
 
R. A. Oldershaw, S. R. Tew, A. M. Russell, K. Meade, R. Hawkins, T. R. McKay, K. R. Brennan, and T. E. Hardingham
Notch Signaling Through Jagged-1 Is Necessary to Initiate Chondrogenesis in Human Bone Marrow Stromal Cells but Must Be Switched off to Complete Chondrogenesis
Stem Cells,
March 1, 2008;
26(3):
666 - 674.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. J. Hayes, D. Tudor, M. A. Nowell, B. Caterson, and C. E. Hughes
Chondroitin Sulfate Sulfation Motifs as Putative Biomarkers for Isolation of Articular Cartilage Progenitor Cells
J. Histochem. Cytochem.,
February 1, 2008;
56(2):
125 - 138.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. D. Murdoch, L. M. Grady, M. P. Ablett, T. Katopodi, R. S. Meadows, and T. E. Hardingham
Chondrogenic Differentiation of Human Bone Marrow Stem Cells in Transwell Cultures: Generation of Scaffold-Free Cartilage
Stem Cells,
November 1, 2007;
25(11):
2786 - 2796.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. English, E. A. Jones, D. Corscadden, K. Henshaw, T. Chapman, P. Emery, and D. McGonagle
A comparative assessment of cartilage and joint fat pad as a potential source of cells for autologous therapy development in knee osteoarthritis
Rheumatology,
November 1, 2007;
46(11):
1676 - 1683.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. J. Hayes, A. Hall, L. Brown, R. Tubo, and B. Caterson
Macromolecular Organization and In Vitro Growth Characteristics of Scaffold-free Neocartilage Grafts
J. Histochem. Cytochem.,
August 1, 2007;
55(8):
853 - 866.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Spagnoli, L. O'Rear, R. L. Chandler, F. Granero-Molto, D. P. Mortlock, A. E. Gorska, J. A. Weis, L. Longobardi, A. Chytil, K. Shimer, et al.
TGF-{beta} signaling is essential for joint morphogenesis
J. Cell Biol.,
July 30, 2007;
177(6):
1105 - 1117.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Abstracts
Ann Rheum Dis,
March 1, 2007;
66(suppl_1):
A6 - A69.
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. A. Rodeo, S. A. Maher, and C. Hidaka
What's New in Orthopaedic Research
J. Bone Joint Surg. Am.,
September 1, 2004;
86(9):
2085 - 2095.
[Full Text]
[PDF]
|
|
|
© The Company of Biologists Ltd 2004
