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JCS ePress online publication date 24 Jul 2007
doi: 10.1242/jcs.009506

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Research Article

A conserved role for kinesin-5 in plant mitosis


Alex Bannigan, Wolf-Rüdiger Scheible, Wolfgang Lukowitz, Carey Fagerstrom, Patricia Wadsworth, Chris Somerville, and Tobias I. Baskin*
* Author for correspondence (e-mail: baskin{at}bio.umass.edu)

The mitotic spindle of vascular plants is assembled and maintained by processes that remain poorly explored at a molecular level. Here, we report that AtKRP125c, one of four kinesin-5 motor proteins in arabidopsis, decorates microtubules throughout the cell cycle and appears to function in both interphase and mitosis. In a temperature-sensitive mutant, interphase cortical microtubules are disorganized at the restrictive temperature and mitotic spindles are massively disrupted, consistent with a defect in the stabilization of anti-parallel microtubules in the spindle midzone, as previously described in kinesin-5 mutants from animals and yeast. AtKRP125c introduced into mammalian epithelial cells by transfection decorates microtubules throughout the cell cycle but is unable to complement the loss of the endogenous kinesin-5 motor (Eg5). These results are among the first reports of any motor with a major role in anastral spindle structure in plants and demonstrate that the conservation of kinesin-5 motor function throughout eukaryotes extends to vascular plants.


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