spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
JCS ePress online publication date 27 Jul 2004
doi: 10.1242/jcs.01283

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jcs.01283v1
117/18/4127    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chiarella, P.
Right arrow Articles by Stefanini, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chiarella, P.
Right arrow Articles by Stefanini, M.

Research Article

Ryanodine receptors are expressed and functionally active in mouse spermatogenic cells and their inhibition interferes with spermatogonial differentiation


Pieranna Chiarella, Rossella Puglisi, Vincenzo Sorrentino, Carla Boitani, and Mario Stefanini*
* Author for correspondence (e-mail: mario.stefanini{at}uniroma1.it)

Ryanodine receptors (RyRs) are intracellular calcium release channels that are highly expressed in striated muscle and neurons but are also detected in several non-excitable cells. We have studied the expression of the three RyR isoforms in male germ cells at different stages of maturation by western blot and RT-PCR. RyR1 was expressed in spermatogonia, pachytene spermatocytes and round spermatids whereas RyR2 was found only in 5- to 10-day-old testis but not in germ cells. RyR3 was not revealed at the protein level, although its mRNA was detected in mixed populations of germ cells. Caffeine, a known agonist of RyRs, was able to induce release of Ca2+ from intracellular stores in spermatogonia, pachytene spermatocytes and round spermatids, but not spermatozoa. Treatment with high doses of ryanodine, which are known to block RyR channel activity, reduced spermatogonial proliferation and induced meiosis in in vitro organ cultures of testis from 7-day-old mice. In conclusion, the results presented here indicate that RyRs are present in germ cells and that calcium mobilization through RyR channels could participate to the regulation of male germ maturation.


This article has been cited by other articles:


Home page
 J AndrolHome page
A. Jankowska, B. Burczynska, T. Duda, J. B. Warchol, and R. K. Sharma
Calcium-Modulated Rod Outer Segment Membrane Guanylate Cyclase Type 1 Transduction Machinery in the Testes
J Androl, January 1, 2007; 28(1): 50 - 58.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. Brailoiu, D. Churamani, V. Pandey, G. C. Brailoiu, F. Tuluc, S. Patel, and N. J. Dun
Messenger-specific Role for Nicotinic Acid Adenine Dinucleotide Phosphate in Neuronal Differentiation
J. Biol. Chem., June 9, 2006; 281(23): 15923 - 15928.
[Abstract] [Full Text] [PDF]

Home page
 Hum Reprod UpdateHome page
C. Jimenez-Gonzalez, F. Michelangeli, C.V. Harper, C.L.R. Barratt, and S.J. Publicover
Calcium signalling in human spermatozoa: a specialized 'toolkit' of channels, transporters and stores
Hum. Reprod. Update, May 1, 2006; 12(3): 253 - 267.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. M. Gibbs, M. J. Scanlon, J. Swarbrick, S. Curtis, E. Gallant, A. F. Dulhunty, and M. K. O'Bryan
The Cysteine-rich Secretory Protein Domain of Tpx-1 Is Related to Ion Channel Toxins and Regulates Ryanodine Receptor Ca2+ Signaling
J. Biol. Chem., February 17, 2006; 281(7): 4156 - 4163.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
C. V. Harper and S. J. Publicover
Reassessing the role of progesterone in fertilization--compartmentalized calcium signalling in human spermatozoa?
Hum. Reprod., October 1, 2005; 20(10): 2675 - 2680.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2004