The fully linked HTML version of this article has now been published.
JCS ePress
online publication date 3 Aug 2004
doi: 10.1242/jcs.01293
Research Article
Trichostatin A-induced histone acetylation causes decondensation of interphase chromatin
Katalin Fejes Tóth,
Tobias A. Knoch,
Malte Wachsmuth,
Monika Frank-Stöhr,
Michael Stöhr,
Christian P. Bacher,
Gabriele Müller,
and
Karsten Rippe*
* Author for correspondence (e-mail: karsten.rippe{at}kip.uni-heidelberg.de)
The effect of trichostatin A (TSA)-induced histone acetylation on the interphase chromatin structure was visualized in vivo with a HeLa cell line stably expressing histone H2A, which was fused to enhanced yellow fluorescent protein. The globally increased histone acetylation caused a reversible decondensation of dense chromatin regions and led to a more homogeneous distribution. These structural changes were quantified by image correlation spectroscopy and by spatially resolved scaling analysis. The image analysis revealed that a chromatin reorganization on a length scale from 200 nm to >1 µm was induced consistent with the opening of condensed chromatin domains containing several Mb of DNA. The observed conformation changes could be assigned to the folding of chromatin during G1 phase by characterizing the effect of TSA on cell cycle progression and developing a protocol that allowed the identification of G1 phase cells on microscope coverslips. An analysis by flow cytometry showed that the addition of TSA led to a significant arrest of cells in S phase and induced apoptosis. The concentration dependence of both processes was studied.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
|
|
 
T. Jegou, I. Chung, G. Heuvelman, M. Wachsmuth, S. M. Gorisch, K. M. Greulich-Bode, P. Boukamp, P. Lichter, and K. Rippe
Dynamics of Telomeres and Promyelocytic Leukemia Nuclear Bodies in a Telomerase-negative Human Cell Line
Mol. Biol. Cell,
April 1, 2009;
20(7):
2070 - 2082.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y.-N. Liu, Y. Liu, H.-J. Lee, Y.-H. Hsu, and J.-H. Chen
Activated Androgen Receptor Downregulates E-Cadherin Gene Expression and Promotes Tumor Metastasis
Mol. Cell. Biol.,
December 1, 2008;
28(23):
7096 - 7108.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Bartova, J. Krejci, A. Harnicarova, G. Galiova, and S. Kozubek
Histone Modifications and Nuclear Architecture: A Review
J. Histochem. Cytochem.,
August 1, 2008;
56(8):
711 - 721.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Brest, M. Gustafsson, A.-K. Mossberg, L. Gustafsson, C. Duringer, A. Hamiche, and C. Svanborg
Histone Deacetylase Inhibitors Promote the Tumoricidal Effect of HAMLET
Cancer Res.,
December 1, 2007;
67(23):
11327 - 11334.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. D. Pajerowski, K. N. Dahl, F. L. Zhong, P. J. Sammak, and D. E. Discher
From the Cover: Physical plasticity of the nucleus in stem cell differentiation
PNAS,
October 2, 2007;
104(40):
15619 - 15624.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Murga, I. Jaco, Y. Fan, R. Soria, B. Martinez-Pastor, M. Cuadrado, S.-M. Yang, M. A. Blasco, A. I. Skoultchi, and O. Fernandez-Capetillo
Global chromatin compaction limits the strength of the DNA damage response
J. Cell Biol.,
September 24, 2007;
178(7):
1101 - 1108.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. R. Saunders and E. Verdin
Ornithine decarboxylase activity in tumor cell lines correlates with sensitivity to cell death induced by histone deacetylase inhibitors.
Mol. Cancer Ther.,
November 1, 2006;
5(11):
2777 - 2785.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. J. Olaharski, Z. Ji, J.-Y. Woo, S. Lim, A. E. Hubbard, L. Zhang, and M. T. Smith
The Histone Deacetylase Inhibitor Trichostatin A Has Genotoxic Effects in Human Lymphoblasts In Vitro
Toxicol. Sci.,
October 1, 2006;
93(2):
341 - 347.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Mazurkiewicz, J. F. Kepert, and K. Rippe
On the Mechanism of Nucleosome Assembly by Histone Chaperone NAP1
J. Biol. Chem.,
June 16, 2006;
281(24):
16462 - 16472.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Mertens, S. Wolf, C. Tschuch, C. Mund, D. Kienle, S. Ohl, P. Schroeter, F. Lyko, H. Dohner, S. Stilgenbauer, et al.
Allelic silencing at the tumor-suppressor locus 13q14.3 suggests an epigenetic tumor-suppressor mechanism
PNAS,
May 16, 2006;
103(20):
7741 - 7746.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. M. Gorisch, M. Wachsmuth, K. F. Toth, P. Lichter, and K. Rippe
Histone acetylation increases chromatin accessibility
J. Cell Sci.,
December 15, 2005;
118(24):
5825 - 5834.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. M. Gorisch, M. Wachsmuth, C. Ittrich, C. P. Bacher, K. Rippe, and P. Lichter
Nuclear body movement is determined by chromatin accessibility and dynamics
PNAS,
September 7, 2004;
101(36):
13221 - 13226.
[Abstract]
[Full Text]
[PDF]
|
|
|
© The Company of Biologists Ltd 2004
