The fully linked HTML version of this article has now been published.
JCS ePress
online publication date 10 Aug 2004
doi: 10.1242/jcs.01307
Research Article
Efficient generation of neural stem cell-like cells from adult human bone marrow stromal cells
Andreas Hermann,
Regina Gastl,
Stefan Liebau,
M. Oana Popa,
Jörg Fiedler,
Bernhard O. Boehm,
Martina Maisel,
Holger Lerche,
Johannes Schwarz,
Rolf Brenner,
and
Alexander Storch*
* Author for correspondence (e-mail: alexander.storch{at}neuro.med.tu-dresden.de)
Clonogenic neural stem cells (NSCs) are self-renewing cells that maintain the capacity to differentiate into brain-specific cell types, and may also replace or repair diseased brain tissue. NSCs can be directly isolated from fetal or adult nervous tissue, or derived from embryonic stem cells. Here, we describe the efficient conversion of human adult bone marrow stromal cells (hMSC) into a neural stem cell-like population (hmNSC, for human marrow-derived NSC-like cells). These cells grow in neurosphere-like structures, express high levels of early neuroectodermal markers, such as the proneural genes NeuroD1, Neurog2, MSl1 as well as otx1 and nestin, but lose the characteristics of mesodermal stromal cells. In the presence of selected growth factors, hmNSCs can be differentiated into the three main neural phenotypes: astroglia, oligodendroglia and neurons. Clonal analysis demonstrates that individual hmNSCs are multipotent and retain the capacity to generate both glia and neurons. Our cell culture system provides a powerful tool for investigating the molecular mechanisms of neural differentiation in adult human NSCs. hmNSCs may therefore ultimately help to treat acute and chronic neurodegenerative diseases.
This article has been cited by other articles:
|
|
|
|
 
T. Trapp, G. Kogler, A. El-Khattouti, R. V. Sorg, M. Besselmann, M. Focking, C. P. Buhrle, I. Trompeter, J. C. Fischer, and P. Wernet
Hepatocyte Growth Factor/c-MET Axis-mediated Tropism of Cord Blood-derived Unrestricted Somatic Stem Cells for Neuronal Injury
J. Biol. Chem.,
November 21, 2008;
283(47):
32244 - 32253.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Shibata, K. Naruse, H. Kamiya, M. Kozakae, M. Kondo, Y. Yasuda, N. Nakamura, K. Ota, T. Tosaki, T. Matsuki, et al.
Transplantation of Bone Marrow-Derived Mesenchymal Stem Cells Improves Diabetic Polyneuropathy in Rats
Diabetes,
November 1, 2008;
57(11):
3099 - 3107.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. L.K. Coles-Takabe, I. Brain, K. A. Purpura, P. Karpowicz, P. W. Zandstra, C. M. Morshead, and D. van der Kooy
Don't Look: Growing Clonal Versus Nonclonal Neural Stem Cell Colonies
Stem Cells,
November 1, 2008;
26(11):
2938 - 2944.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. L. Troyer and M. L. Weiss
Concise Review: Wharton's Jelly-Derived Cells Are a Primitive Stromal Cell Population
Stem Cells,
March 1, 2008;
26(3):
591 - 599.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Lu, X. Chen, C.-W. Zhang, W.-L. Yang, Y.-J. Wu, L. Sun, L.-M. Bai, X.-S. Gu, S. Ahmed, G. S. Dawe, et al.
Morphological and Functional Characterization of Predifferentiation of Myelinating Glia-Like Cells from Human Bone Marrow Stromal Cells Through Activation of F3/Notch Signaling in Mouse Retina
Stem Cells,
February 1, 2008;
26(2):
580 - 590.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Maisel, A. Herr, J. Milosevic, A. Hermann, H.-J. Habisch, S. Schwarz, M. Kirsch, G. Antoniadis, R. Brenner, S. Hallmeyer-Elgner, et al.
Transcription Profiling of Adult and Fetal Human Neuroprogenitors Identifies Divergent Paths to Maintain the Neuroprogenitor Cell State
Stem Cells,
May 1, 2007;
25(5):
1231 - 1240.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Kan, E. Melamed, D. Offen, and P. Green
Docosahexaenoic acid and arachidonic acid are fundamental supplements for the induction of neuronal differentiation
J. Lipid Res.,
March 1, 2007;
48(3):
513 - 517.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Yoshida, S. Shimmura, N. Nagoshi, K. Fukuda, Y. Matsuzaki, H. Okano, and K. Tsubota
Isolation of Multipotent Neural Crest-Derived Stem Cells from the Adult Mouse Cornea
Stem Cells,
December 1, 2006;
24(12):
2714 - 2722.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. P. Croft and S. A. Przyborski
Formation of neurons by non-neural adult stem cells: potential mechanism implicates an artifact of growth in culture.
Stem Cells,
August 1, 2006;
24(8):
1841 - 1851.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Pacary, H. Legros, S. Valable, P. Duchatelle, M. Lecocq, E. Petit, O. Nicole, and M. Bernaudin
Synergistic effects of CoCl2 and ROCK inhibition on mesenchymal stem cell differentiation into neuron-like cells
J. Cell Sci.,
July 1, 2006;
119(13):
2667 - 2678.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Roybon, Z. Ma, F. Asztely, A. Fosum, S. E. W. Jacobsen, P. Brundin, and J.-Y. Li
Failure of Transdifferentiation of Adult Hematopoietic Stem Cells into Neurons
Stem Cells,
June 1, 2006;
24(6):
1594 - 1604.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Hermann, M. Maisel, F. Wegner, S. Liebau, D.-W. Kim, M. Gerlach, J. Schwarz, K.-S. Kim, and A. Storch
Multipotent Neural Stem Cells from the Adult Tegmentum with Dopaminergic Potential Develop Essential Properties of Functional Neurons
Stem Cells,
April 1, 2006;
24(4):
949 - 964.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Bertani, P. Malatesta, G. Volpi, P. Sonego, and R. Perris
Neurogenic potential of human mesenchymal stem cells revisited: analysis by immunostaining, time-lapse video and microarray
J. Cell Sci.,
September 1, 2005;
118(17):
3925 - 3936.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Sun, L. Buzanska, K. Domanska-Janik, R. J. Salvi, and M. K. Stachowiak
Voltage-Sensitive and Ligand-Gated Channels in Differentiating Neural Stem-Like Cells Derived from the Nonhematopoietic Fraction of Human Umbilical Cord Blood
Stem Cells,
August 1, 2005;
23(7):
931 - 945.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Zipori
The Stem State: Plasticity Is Essential, Whereas Self-Renewal and Hierarchy Are Optional
Stem Cells,
June 1, 2005;
23(6):
719 - 726.
[Abstract]
[Full Text]
[PDF]
|
|
|
© The Company of Biologists Ltd 2004
