The fully linked HTML version of this article has now been published.
JCS ePress
online publication date 16 Nov 2004
doi: 10.1242/jcs.01532
Research Article
The lamin CxxM motif promotes nuclear membrane growth
Kristina Prüfert,
Annette Vogel,
and
Georg Krohne*
* Author for correspondence (e-mail: krohne{at}biozentrum.uni-wuerzburg.de)
We analyzed the influence of lamins on nuclear envelope growth in cultured Xenopus A6 cells by the overexpression of human lamin A, Xenopus and zebrafish lamins B2 and Drosophila lamins Dm0 and C as GFP fusion proteins. Lamins containing a CxxM motif in their primary sequence (lamins A, B2, Dm0) induced the formation of lobulated nuclei with multi-membrane-layered, highly folded nuclear membranes and intranuclear membrane assemblies, as observed by electron microscopy. Such morphological alterations were not observed with Drosophila lamin C, a lamin without this motif or with a lamin B2 mutant (B2-SxxM) where the cysteine of the CxxM motif is replaced by a serine. Drosophila lamin C mutants containing a CxxM motif behaved like B-type lamins thus confirming that this tetrapeptide is directly involved in the morphological changes we observed. Nuclear membrane proliferation could also be induced by lamin B2 in COS-7 cells and in zebrafish embryos but not by human lamin A in COS-7 cells. We speculate that the human lamin A is incompletely processed in Xenopus A6 cells and therefore behaves in this cell line like a B-type lamin. Our results indicate that the CxxM motif of B-type lamins has a dual function: it mediates lamin targeting to the inner nuclear membrane thereby promoting nuclear membrane growth.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
|
|
 
N. Sabherwal, A. Tsutsui, S. Hodge, J. Wei, A. D. Chalmers, and N. Papalopulu
The apicobasal polarity kinase aPKC functions as a nuclear determinant and regulates cell proliferation and fate during Xenopus primary neurogenesis
Development,
August 15, 2009;
136(16):
2767 - 2777.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S.-T. Lin and Y.-H. Fu
miR-23 regulation of lamin B1 is crucial for oligodendrocyte development and myelination
Dis. Model. Mech.,
March 1, 2009;
2(3-4):
178 - 188.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Guffanti, N. Kittur, Z. N. Brodt, A. J. Polotsky, S. M. Kuokkanen, D. S. Heller, S. L. Young, N. Santoro, and U. T. Meier
Nuclear pore complex proteins mark the implantation window in human endometrium
J. Cell Sci.,
June 15, 2008;
121(12):
2037 - 2045.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Luke, H. Zaim, I. Karakesisoglou, V. M. Jaeger, L. Sellin, W. Lu, M. Schneider, S. Neumann, A. Beijer, M. Munck, et al.
Nesprin-2 Giant (NUANCE) maintains nuclear envelope architecture and composition in skin
J. Cell Sci.,
June 1, 2008;
121(11):
1887 - 1898.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. W. Goldberg, I. Huttenlauch, C. J. Hutchison, and R. Stick
Filaments made from A- and B-type lamins differ in structure and organization
J. Cell Sci.,
January 15, 2008;
121(2):
215 - 225.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Gehrig, R. B. Cornell, and N. D. Ridgway
Expansion of the Nucleoplasmic Reticulum Requires the Coordinated Activity of Lamins and CTP:Phosphocholine Cytidylyltransferase {alpha}
Mol. Biol. Cell,
January 1, 2008;
19(1):
237 - 247.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. G. Klupp, H. Granzow, W. Fuchs, G. M. Keil, S. Finke, and T. C. Mettenleiter
Vesicle formation from the nuclear membrane is induced by coexpression of two conserved herpesvirus proteins
PNAS,
April 24, 2007;
104(17):
7241 - 7246.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Snyers, S. Vlcek, T. Dechat, D. Skegro, B. Korbei, A. Gajewski, O. Mayans, C. Schofer, and R. Foisner
Lamina-associated Polypeptide 2-{alpha} Forms Homo-trimers via Its C Terminus, and Oligomerization Is Unaffected by a Disease-causing Mutation
J. Biol. Chem.,
March 2, 2007;
282(9):
6308 - 6315.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. L.R.M. Verstraeten, J. L.V. Broers, M. A.M. van Steensel, S. Zinn-Justin, F. C.S. Ramaekers, P. M. Steijlen, M. Kamps, H. J.H. Kuijpers, D. Merckx, H. J.M. Smeets, et al.
Compound heterozygosity for mutations in LMNA causes a progeria syndrome without prelamin A accumulation
Hum. Mol. Genet.,
August 15, 2006;
15(16):
2509 - 2522.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. L. V. Broers, F. C. S. Ramaekers, G. Bonne, R. B. Yaou, and C. J. Hutchison
Nuclear lamins: laminopathies and their role in premature ageing.
Physiol Rev,
July 1, 2006;
86(3):
967 - 1008.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Delbarre, M. Tramier, M. Coppey-Moisan, C. Gaillard, J.-C. Courvalin, and B. Buendia
The truncated prelamin A in Hutchinson-Gilford progeria syndrome alters segregation of A-type and B-type lamin homopolymers
Hum. Mol. Genet.,
April 1, 2006;
15(7):
1113 - 1122.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. McClintock, L. B. Gordon, and K. Djabali
Hutchinson-Gilford progeria mutant lamin A primarily targets human vascular cells as detected by an anti-Lamin A G608G antibody
PNAS,
February 14, 2006;
103(7):
2154 - 2159.
[Abstract]
[Full Text]
[PDF]
|
|
|
© The Company of Biologists Ltd 2004
