spacer gif spacer gif spacer gif spacer gif Propose a workshop for 2011 spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
JCS ePress online publication date 15 Dec 2004
doi: 10.1242/jcs.01553

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jcs.01553v1
118/1/27    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Araya, R.
Right arrow Articles by Sáez, J. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Araya, R.
Right arrow Articles by Sáez, J. C.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research Article

Expression of connexins during differentiation and regeneration of skeletal muscle: functional relevance of connexin43


Roberto Araya*, Dominik Eckardt, Stephan Maxeiner, Olaf Krüger, Martin Theis, Klaus Willecke, and Juan C. Sáez
* Author for correspondence (e-mail: ra2174{at}columbia.edu)

The molecular mechanisms regulating skeletal muscle regeneration and differentiation are not well understood. We analyzed the expression of connexins (Cxs) 40, 43 and 45 in normal and regenerating tibialis anterior muscle and in primary cultures of differentiating myoblasts in adult and newborn mice, respectively. Cxs 45 and 43, but not 40, were strongly expressed in normal muscle and their expression was upregulated during regeneration. Furthermore, the functional role of Cx43 during differentiation and regeneration was examined after induced deletion of Cx43 in transgenic mice. In vivo, the inducible deletion of Cx43 delayed the formation of myofibers and prolonged the expression of myogenin during regeneration. In primary cultures of satellite cell-derived myoblasts, induced deletion of Cx43 led to decreased expression of myogenin and MyoD, dye coupling, creatine kinase activity and myoblast fusion. Thus, the expression of Cx45 and Cx43 is upregulated during skeletal muscle regeneration and Cx43 is required for normal myogenesis in vitro and adult muscle regeneration in vivo.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 DevelopmentHome page
F. J. Conti, S. J. Monkley, M. R. Wood, D. R. Critchley, and U. Muller
Talin 1 and 2 are required for myoblast fusion, sarcomere assembly and the maintenance of myotendinous junctions
Development, November 1, 2009; 136(21): 3597 - 3606.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
A. Trovato-Salinaro, N. Belluardo, M. Frinchi, J. von Maltzahn, K. Willecke, D. F. Condorelli, and G. Mudo
Regulation of connexin gene expression during skeletal muscle regeneration in the adult rat
Am J Physiol Cell Physiol, March 1, 2009; 296(3): C593 - C606.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
C. Anderson, H. Catoe, and R. Werner
MIR-206 regulates connexin43 expression during skeletal muscle development
Nucleic Acids Res., November 6, 2006; 34(20): 5863 - 5871.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
Y.-H. Choi, C. Stamm, P. E. Hammer, K. F. Kwaku, J. J. Marler, I. Friehs, M. Jones, C. M. Rader, N. Roy, M.-T. Eddy, et al.
Cardiac Conduction through Engineered Tissue
Am. J. Pathol., July 1, 2006; 169(1): 72 - 85.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2004