spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
JCS ePress online publication date 23 Nov 2004
doi: 10.1242/jcs.01561

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jcs.01561v1
117/26/6413    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Merino-Trigo, A.
Right arrow Articles by Gleeson, P. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Merino-Trigo, A.
Right arrow Articles by Gleeson, P. A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research Article

Sorting nexin 5 is localized to a subdomain of the early endosomes and is recruited to the plasma membrane following EGF stimulation


Ana Merino-Trigo, Markus C. Kerr, Fiona Houghton, Anna Lindberg, Christina Mitchell, Rohan D. Teasdale, and Paul A. Gleeson*
* Author for correspondence (e-mail: pgleeson{at}unimelb.edu.au)

Sorting nexins are a large family of proteins that contain the phosphoinositide-binding Phox homology (PX) domain. A number of sorting nexins are known to bind to PtdIns(3)P, which mediates their localization to membranes of the endocytic pathway. We show here that sorting nexin 5 (SNX5) can be recruited to two distinct membrane compartments. In non-stimulated cells, the PX domain was independently targeted to endosomal structures and colocalized with full-length SNX5. The membrane binding of the PX domain was inhibited by the PI 3-kinase inhibitor, wortmannin. Although SNX5 colocalized with a fluid-phase marker and was found predominantly within a PtdIns(3)P-rich endosomal domain, very little colocalization was observed between SNX5 and the PtdIns(3)P-binding protein, EEA1. Using liposome-based binding assays, we have shown that the PX domain of SNX5 interacts not only with PtdIns(3)P but also with PtdIns(3,4)P2. In response to EGF stimulation, either the SNX5-PX domain or full-length SNX5 was rapidly recruited to the plasma membrane. The localization of SNX1, which does not bind PtdIns(3,4)P2, was unaffected by EGF signalling. Therefore, SNX5 is localized to a subdomain of the early endosome distinct from EEA1 and, following EGF stimulation and elevation of PtdIns(3,4)P2, is also transiently recruited to the plasma membrane. These results indicate that SNX5 may have functions not only associated with endosomal sorting but also with the phosphoinositide-signalling pathway.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
L. M. I. Koharudin, W. Furey, H. Liu, Y.-J. Liu, and A. M. Gronenborn
The Phox Domain of Sorting Nexin 5 Lacks Phosphatidylinositol 3-Phosphate (PtdIns(3)P) Specificity and Preferentially Binds to Phosphatidylinositol 4,5-Bisphosphate (PtdIns(4,5)P2)
J. Biol. Chem., August 28, 2009; 284(35): 23697 - 23707.
[Abstract] [Full Text] [PDF]

Home page
 Mol PlantHome page
N. Q. Phan, S.-J. Kim, and D. C. Bassham
Overexpression of Arabidopsis Sorting Nexin AtSNX2b Inhibits Endocytic Trafficking to the Vacuole
Mol Plant, November 1, 2008; 1(6): 961 - 976.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
S. Hara, E. Kiyokawa, S.-i. Iemura, T. Natsume, T. Wassmer, P. J. Cullen, H. Hiai, and M. Matsuda
The DHR1 Domain of DOCK180 Binds to SNX5 and Regulates Cation-independent Mannose 6-phosphate Receptor Transport
Mol. Biol. Cell, September 1, 2008; 19(9): 3823 - 3835.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
N. J. D. McLaughlin, A. Banerjee, S. Y. Khan, J. L. Lieber, M. R. Kelher, F. Gamboni-Robertson, F. R. Sheppard, E. E. Moore, G. W. Mierau, D. J. Elzi, et al.
Platelet-Activating Factor-Mediated Endosome Formation Causes Membrane Translocation of p67phox and p40phox That Requires Recruitment and Activation of p38 MAPK, Rab5a, and Phosphatidylinositol 3-Kinase in Human Neutrophils
J. Immunol., June 15, 2008; 180(12): 8192 - 8203.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
M. V. Bujny, P. A. Ewels, S. Humphrey, N. Attar, M. A. Jepson, and P. J. Cullen
Sorting nexin-1 defines an early phase of Salmonella-containing vacuole-remodeling during Salmonella infection
J. Cell Sci., June 15, 2008; 121(12): 2027 - 2036.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
T. Wassmer, N. Attar, M. V. Bujny, J. Oakley, C. J. Traer, and P. J. Cullen
A loss-of-function screen reveals SNX5 and SNX6 as potential components of the mammalian retromer
J. Cell Sci., January 1, 2007; 120(1): 45 - 54.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
M. L. Gifford, F. C. Robertson, D. C. Soares, and G. C. Ingram
ARABIDOPSIS CRINKLY4 Function, Internalization, and Turnover Are Dependent on the Extracellular Crinkly Repeat Domain
PLANT CELL, April 1, 2005; 17(4): 1154 - 1166.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2004