spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
JCS ePress online publication date 22 Jan 2008
doi: 10.1242/jcs.015826

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jcs.015826v1
121/4/428    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tsao, C.-C.
Right arrow Articles by Gorovsky, M. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tsao, C.-C.
Right arrow Articles by Gorovsky, M. A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research Article

Tetrahymena IFT122A is not essential for cilia assembly but plays a role in returning IFT proteins from the ciliary tip to the cell body


Che-Chia Tsao and Martin A. Gorovsky*
* Author for correspondence (e-mail: goro{at}mail.rochester.edu)

Intraflagellar transport (IFT) moves multiple protein particles composed of two biochemically distinct complexes, IFT-A and IFT-B, bi-directionally within cilia and is essential for cilia assembly and maintenance. We identified an ORF from the Tetrahymena macronuclear genome sequence, encoding IFT122A, an ortholog of an IFT-A complex protein. Tetrahymena IFT122A is induced during cilia regeneration, and epitope-tagged Ift122Ap could be detected in isolated cilia. IFT122A knockout cells still assembled cilia, albeit with lower efficiency, and could regenerate amputated cilia. Ift172p and Ift88p, two IFT-B complex proteins that localized mainly to basal bodies and along the cilia in wild-type cells, became preferentially enriched at the ciliary tips in IFT122A knockout cells. Our results indicate that Tetrahymena IFT122A is not required for anterograde transport-dependent ciliary assembly but plays a role in returning IFT proteins from the ciliary tip to the cell body.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 Eukaryot CellHome page
D. Dave, D. Wloga, N. Sharma, and J. Gaertig
DYF-1 Is Required for Assembly of the Axoneme in Tetrahymena thermophila
Eukaryot. Cell, September 1, 2009; 8(9): 1397 - 1406.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
K. Huang, D. R. Diener, and J. L. Rosenbaum
The ubiquitin conjugation system is involved in the disassembly of cilia and flagella
J. Cell Biol., August 24, 2009; 186(4): 601 - 613.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
L. Hao and J. M. Scholey
Intraflagellar transport at a glance
J. Cell Sci., April 1, 2009; 122(7): 889 - 892.
[Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
V. Rajagopalan, A. Subramanian, D. E. Wilkes, D. G. Pennock, and D. J. Asai
Dynein-2 Affects the Regulation of Ciliary Length but Is Not Required for Ciliogenesis in Tetrahymena thermophila
Mol. Biol. Cell, January 1, 2009; 20(2): 708 - 720.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2008