The fully linked HTML version of this article has now been published.
JCS ePress
online publication date 19 Apr 2005
doi: 10.1242/jcs.02340
Research Article
PECAM-1, 
6 integrins and neutrophil elastase co-operate in mediating neutrophil transmigration
Shijun Wang,
John P. Dangerfield,
Rebecca E. Young,
and
Sussan Nourshargh*
* Author for correspondence (e-mail: s.nourshargh{at}imperial.ac.uk)
The heterogeneous nature of the perivascular basement membrane (composed primarily of laminin and collagen type IV) suggests the existence of an elaborate array of adhesive interactions and possibly proteolytic events in leukocyte migration through this barrier. In this context, blockade of 
6 integrins (laminin receptors), neutrophil elastase (NE) or both inhibited neutrophil migration through interleukin-1
(IL-1)-stimulated mouse cremasteric venules, as observed by intravital microscopy. Furthermore, analysis of tissues by confocal microscopy indicated a synergistic role for 6 integrins and NE in mediating neutrophil migration through the perivascular basement membrane. Using a combined in vitro and in vivo experimental approach, the findings of this study also suggest that 6 integrins and NE are mobilized from intracellular stores to the cell surface of transmigrating mouse neutrophils, although these events occur via mechanisms dependent on and independent of platelet/endothelial-cell adhesion molecule 1 (PECAM-1, CD31), respectively. Despite different regulatory mechanisms, blockade of 6 integrins or NE inhibited migration of murine neutrophils through laminin-coated filters in vitro. Collectively, the findings suggest that, whereas regulation of the expression of 6 integrins and NE occur via different adhesive mechanisms, these molecules might act in a co-operative manner in mediating neutrophil migration through venular walls, in particular the perivascular basement membrane.
This article has been cited by other articles:
|
|
|
|
 
A. S. Cowburn, A. M. Condliffe, N. Farahi, C. Summers, and E. R. Chilvers
Advances in Neutrophil Biology: Clinical Implications
Chest,
September 1, 2008;
134(3):
606 - 612.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Sugimoto, T. Rui, M. Yang, S. Bharwani, O. Handa, N. Yoshida, T. Yoshikawa, and P. R. Kvietys
Points of Control Exerted along the Macrophage-Endothelial Cell-Polymorphonuclear Neutrophil Axis by PECAM-1 in the Innate Immune Response of Acute Colonic Inflammation
J. Immunol.,
August 1, 2008;
181(3):
2145 - 2154.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. M. Kaynar, A. M. Houghton, E. H. Lum, B. R. Pitt, and S. D. Shapiro
Neutrophil Elastase Is Needed for Neutrophil Emigration into Lungs in Ventilator-Induced Lung Injury
Am. J. Respir. Cell Mol. Biol.,
July 1, 2008;
39(1):
53 - 60.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Mydel, J. M. Shipley, T. L. Adair-Kirk, D. G. Kelley, T. J. Broekelmann, R. P. Mecham, and R. M. Senior
Neutrophil Elastase Cleaves Laminin-332 (Laminin-5) Generating Peptides That Are Chemotactic for Neutrophils
J. Biol. Chem.,
April 11, 2008;
283(15):
9513 - 9522.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. A. Reichel, M. Rehberg, P. Bihari, C. M. Moser, S. Linder, A. Khandoga, and F. Krombach
Gelatinases mediate neutrophil recruitment in vivo: evidence for stimulus specificity and a critical role in collagen IV remodeling
J. Leukoc. Biol.,
April 1, 2008;
83(4):
864 - 874.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Woodfin, M.-B. Voisin, and S. Nourshargh
PECAM-1: A Multi-Functional Molecule in Inflammation and Vascular Biology
Arterioscler. Thromb. Vasc. Biol.,
December 1, 2007;
27(12):
2514 - 2523.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Woodfin, C. A. Reichel, A. Khandoga, M. Corada, M.-B. Voisin, C. Scheiermann, D. O. Haskard, E. Dejana, F. Krombach, and S. Nourshargh
JAM-A mediates neutrophil transmigration in a stimulus-specific manner in vivo: evidence for sequential roles for JAM-A and PECAM-1 in neutrophil transmigration
Blood,
September 15, 2007;
110(6):
1848 - 1856.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. C. McGown and Z. L. S. Brookes
Beneficial effects of statins on the microcirculation during sepsis: the role of nitric oxide
Br. J. Anaesth.,
February 1, 2007;
98(2):
163 - 175.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Nourshargh, F. Krombach, and E. Dejana
The role of JAM-A and PECAM-1 in modulating leukocyte infiltration in inflamed and ischemic tissues
J. Leukoc. Biol.,
October 1, 2006;
80(4):
714 - 718.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M.-T. Huang, K. Y. Larbi, C. Scheiermann, A. Woodfin, N. Gerwin, D. O. Haskard, and S. Nourshargh
ICAM-2 mediates neutrophil transmigration in vivo: evidence for stimulus specificity and a role in PECAM-1-independent transmigration
Blood,
June 15, 2006;
107(12):
4721 - 4727.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Wang, M.-B. Voisin, K. Y. Larbi, J. Dangerfield, C. Scheiermann, M. Tran, P. H. Maxwell, L. Sorokin, and S. Nourshargh
Venular basement membranes contain specific matrix protein low expression regions that act as exit points for emigrating neutrophils
J. Exp. Med.,
June 12, 2006;
203(6):
1519 - 1532.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. J. Evans, A. McDowall, P. C. Taylor, N. Hogg, D. O. Haskard, and R. C. Landis
Shedding of lymphocyte function-associated antigen-1 (LFA-1) in a human inflammatory response
Blood,
May 1, 2006;
107(9):
3593 - 3599.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Cernuda-Morollon and A. J. Ridley
Rho GTPases and Leukocyte Adhesion Receptor Expression and Function in Endothelial Cells
Circ. Res.,
March 31, 2006;
98(6):
757 - 767.
[Abstract]
[Full Text]
[PDF]
|
|
|
© The Company of Biologists Ltd 2005
