spacer gif spacer gif spacer gif spacer gif Propose a workshop for 2011 spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
JCS ePress online publication date 19 Feb 2008
doi: 10.1242/jcs.023903

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jcs.023903v1
121/6/762    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Smyth, J. T.
Right arrow Articles by Putney, Jr, J. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Smyth, J. T.
Right arrow Articles by Putney, Jr, J. W.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research Article

Ca2+-store-dependent and -independent reversal of Stim1 localization and function


Jeremy T. Smyth, Wayne I. DeHaven, Gary S. Bird, and James W. Putney, Jr*
* Author for correspondence (e-mail: putney{at}niehs.nih.gov)

Stim1 responds to depletion of ER Ca2+ stores by rearranging from tubular structures throughout the ER into punctate structures near the plasma membrane, where it activates Orai store-operated Ca2+ entry (SOCE) channels. However, the mechanism and structural determinants of the localization and reversal of Stim1 puncta formation are poorly understood. Using HEK293 cells expressing Stim1 tagged with enhanced yellow fluorescent protein (EYFP-Stim1), we show that the basis for SOCE termination is the reversal of the punctate Stim1 localization, which absolutely depends on SOCE-dependent store refilling. We also describe rapid, store-independent reversal of EYFP-Stim1 punctae by the ML-9 inhibitor of myosin-light-chain kinase (MLCK). ML-9 similarly inhibited SOCE and the Ca2+-release-activated Ca2+ (CRAC) current. Reversal by ML-9 resulted in full re-establishment of the tubular EYFP-Stim1 localization. A constitutively active EF-hand mutant of EYFP-Stim1 was also reversed by ML-9, regardless of the Ca2+ store content. Inhibition by ML-9 was not due to MLCK inhibition as other inhibitors of MLCK had no effect. Finally, we provide evidence that EYFP-Stim1 punctae form in specific predetermined cellular loci. We conclude that SOCE is tightly coupled to formation of Stim1 puncta, and both SOCE and puncta formation involve a dynamic, reversible signaling complex that probably consists of components in addition to Stim1 and Orai channels.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
X. Deng, Y. Wang, Y. Zhou, J. Soboloff, and D. L. Gill
STIM and Orai: Dynamic Intermembrane Coupling to Control Cellular Calcium Signals
J. Biol. Chem., August 21, 2009; 284(34): 22501 - 22505.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
M. Potier, J. C. Gonzalez, R. K. Motiani, I. F. Abdullaev, J. M. Bisaillon, H. A. Singer, and M. Trebak
Evidence for STIM1- and Orai1-dependent store-operated calcium influx through ICRAC in vascular smooth muscle cells: role in proliferation and migration
FASEB J, August 1, 2009; 23(8): 2425 - 2437.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
N. Scrimgeour, T. Litjens, L. Ma, G. J. Barritt, and G. Y. Rychkov
Properties of Orai1 mediated store-operated current depend on the expression levels of STIM1 and Orai1 proteins
J. Physiol., June 15, 2009; 587(12): 2903 - 2918.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
W. I. DeHaven, B. F. Jones, J. G. Petranka, J. T. Smyth, T. Tomita, G. S. Bird, and J. W. Putney Jr
TRPC channels function independently of STIM1 and Orai1
J. Physiol., May 15, 2009; 587(10): 2275 - 2298.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
T. Morita, A. Tanimura, Y. Baba, T. Kurosaki, and Y. Tojyo
A Stim1-dependent, noncapacitative Ca2+-entry pathway is activated by B-cell-receptor stimulation and depletion of Ca2+
J. Cell Sci., April 15, 2009; 122(8): 1220 - 1228.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
A. C. L. Martin, D. Willoughby, A. Ciruela, L.-J. Ayling, M. Pagano, S. Wachten, A. Tengholm, and D. M. F. Cooper
Capacitative Ca2+ Entry via Orai1 and Stromal Interacting Molecule 1 (STIM1) Regulates Adenylyl Cyclase Type 8
Mol. Pharmacol., April 1, 2009; 75(4): 830 - 842.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Y. Liao, N. W. Plummer, M. D. George, J. Abramowitz, M. X. Zhu, and L. Birnbaumer
A role for Orai in TRPC-mediated Ca2+ entry suggests that a TRPC:Orai complex may mediate store and receptor operated Ca2+ entry
PNAS, March 3, 2009; 106(9): 3202 - 3206.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
T. Lyubchenko, J. P. Nielsen, S. M. Miller, G. A. Liubchenko, and V. M. Holers
Role of initial protein phosphorylation events and localized release-activated calcium influx in B cell antigen receptor signaling
J. Leukoc. Biol., February 1, 2009; 85(2): 298 - 309.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
D. Poburko, C.-H. Liao, C. van Breemen, and N. Demaurex
Mitochondrial Regulation of Sarcoplasmic Reticulum Ca2+ Content in Vascular Smooth Muscle Cells
Circ. Res., January 2, 2009; 104(1): 104 - 112.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
N. Calloway, M. Vig, J.-P. Kinet, D. Holowka, and B. Baird
Molecular Clustering of STIM1 with Orai1/CRACM1 at the Plasma Membrane Depends Dynamically on Depletion of Ca2+ Stores and on Electrostatic Interactions
Mol. Biol. Cell, January 1, 2009; 20(1): 389 - 399.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
T. Gwozdz, J. Dutko-Gwozdz, V. Zarayskiy, K. Peter, and V. M. Bolotina
How strict is the correlation between STIM1 and Orai1 expression, puncta formation, and ICRAC activation?
Am J Physiol Cell Physiol, November 1, 2008; 295(5): C1133 - C1140.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
W. I. DeHaven, J. T. Smyth, R. R. Boyles, G. S. Bird, and J. W. Putney Jr.
Complex Actions of 2-Aminoethyldiphenyl Borate on Store-operated Calcium Entry
J. Biol. Chem., July 11, 2008; 283(28): 19265 - 19273.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
C. Peinelt, A. Lis, A. Beck, A. Fleig, and R. Penner
2-Aminoethoxydiphenyl borate directly facilitates and indirectly inhibits STIM1-dependent gating of CRAC channels
J. Physiol., July 1, 2008; 586(13): 3061 - 3073.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2008