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JCS ePress online publication date 17 Jan 2006
doi: 10.1242/jcs.02730

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Research Article

CD9 controls the formation of clusters that contain tetraspanins and the integrin {alpha}6{beta}1, which are involved in human and mouse gamete fusion


Ahmed Ziyyat, Eric Rubinstein, Frédérique Monier-Gavelle, Virginie Barraud, Olivier Kulski, Michel Prenant, Claude Boucheix, Morgane Bomsel, and Jean-Philippe Wolf*
* Author for correspondence (e-mail: jean-philippe.wolf{at}jvr.ap-hop-paris.fr)

The process of gamete fusion has been largely studied in the mouse and has revealed the crucial role of the tetraspanin CD9. By contrast, human gamete fusion remains largely unknown. We now show that an anti-{alpha}6 integrin mAb (GoH3) strongly inhibited human sperm-egg fusion in human zona-free eggs. Furthermore, a mAb directed against CD151, a tetraspanin known to associate with {alpha}6{beta}1, partially inhibited sperm-egg fusion. By contrast, the addition of an anti-CD9 mAb to zona free eggs had no effect. The integrin {alpha}6{beta}1, CD151 and CD9 tetraspanins were evenly distributed on human zona-intact oocytes. On zona-free eggs, the integrin {alpha}6{beta}1 and tetraspanin CD151 patched and co-localized but the tetraspanin CD9 remained unchanged. CD9 mAb prevented {alpha}6{beta}1 integrin clustering and gamete fusion when added prior to, but not after, zona removal. Antibody-mediated aggregation of integrin {alpha}6{beta}1 yielded patches that were bigger and more heterogeneous in mouse oocytes lacking CD9. Moreover, a strong labelling of {alpha}6{beta}1 could be observed at the sperm entry point. Altogether, these data show that CD9 controls the redistribution of some membrane proteins including the {alpha}6{beta}1 integrin into clusters that may be necessary for gamete fusion.
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